Construct: ORF TRCN0000469374
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014208.1_s317c1
- Derived from:
- ccsbBroadEn_03124
- DNA Barcode:
- ATCCCAATCCGCCCCATCTCATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PILRB (29990)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469374
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29990 | PILRB | paired immunoglobin like ty... | NM_178238.3 | 100% | 100% | |
| 2 | human | 29990 | PILRB | paired immunoglobin like ty... | NM_001371931.1 | 79.9% | 79.9% | 656_826del |
| 3 | human | 29992 | PILRA | paired immunoglobin like ty... | NM_178272.2 | 72.8% | 60.4% | (many diffs) |
| 4 | human | 29992 | PILRA | paired immunoglobin like ty... | NM_178273.2 | 68% | 62.3% | (many diffs) |
| 5 | human | 29992 | PILRA | paired immunoglobin like ty... | NM_013439.3 | 67.7% | 60.3% | (many diffs) |
| 6 | human | 29992 | PILRA | paired immunoglobin like ty... | XM_024446739.1 | 67.7% | 60.3% | (many diffs) |
| 7 | human | 101752399 | STAG3L5P-PVRIG2P-PILRB | STAG3L5P-PVRIG2P-PILRB read... | NR_036570.1 | 19.6% | (many diffs) | |
| 8 | human | 101752399 | STAG3L5P-PVRIG2P-PILRB | STAG3L5P-PVRIG2P-PILRB read... | NR_036569.1 | 18.3% | 1_2545del;3227_3705del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 747
- ORF length:
- 681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tcggcccctg ctgctgcccc tgctgctcct gctgcagccg ccagcatttc 121 tgcagcctgg tggctccaca ggatctggtc caagctacct ttatggggtc actcaaccaa 181 aacacctctc agcctccatg ggtggctctg tggaaatccc cttctccttc tattacccct 241 gggagttagc catagttccc aacgtgagaa tatcctggag acggggccac ttccacgggc 301 agtccttcTA CAGCACAAGG CCGCCTTCCA TTCACAAGGA TTATGTGAAC CGGCTCTTTC 361 TGAACTGGAC AGAGGGTCAG GAGAGCGGCT TCCTCAGGAT CTCAAACCTG CGGAAGGAGG 421 ACCAGTCTGT GTATTTCTGC CGAGTCGAGC TGGACACCCG GAGATCAGGG AGGCAGCAGT 481 TGCAGTCCAT CAAGGGGACC AAACTCACCA TCACCCAGGC TGTCACAACC ACCACCACCT 541 GGAGGCCCAG CAGCACAACC ACCATAGCCG GCCTCAGGGT CACAGAAAGC AAAGGGCACT 601 CAGAATCATG GCACCTAAGT CTGGACACTG CCATCAGGGT TGCATTGGCT GTCGCTGTGC 661 TCAAAACTGT CATTTTGGGA CTGCTGTGCC TCCTCCTCCT GTGGTGGAGG AGAAGGAAAG 721 GTAGCAGGGC GCCAAGCAGT GACTTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATCCCAAT 901 CCGCCCCATC TCATACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 961 aagatt