Construct: ORF TRCN0000469385
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007533.1_s317c1
- Derived from:
- ccsbBroadEn_13923
- DNA Barcode:
- GCCCTAACTTTAGAAGTTCCTTTC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1CB (5500)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469385
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5500 | PPP1CB | protein phosphatase 1 catal... | NM_002709.3 | 99.7% | 99% | 201A>G;970delC |
| 2 | human | 5500 | PPP1CB | protein phosphatase 1 catal... | NM_206876.1 | 99.7% | 99% | 201A>G;970delC |
| 3 | mouse | 19046 | Ppp1cb | protein phosphatase 1, cata... | NM_172707.3 | 94.3% | 99% | (many diffs) |
| 4 | mouse | 19046 | Ppp1cb | protein phosphatase 1, cata... | XM_006503780.1 | 57% | 60.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacggggag ctgaacgtgg acagcctcat cacccggctg ctggaggtac 121 gaggatgtcg tccaggaaag attgtgcaga tgactgaagc agaagttcga ggcttatgta 181 tcaagtctcg ggagatcttt ctcagccagc ctattctttt ggaattggaa gcaccgctga 241 aaatttgtgg agatattcat ggacagtata cagatttact gagattattt gaatatggag 301 gtttcccacc agaagccaac tatcttttct taggagatta tgtggacaga ggaaagcagt 361 ctttggaaac catttgtttg ctattggctt ataaaatcaa atatccagag aacttctttc 421 tcttaagagg aaaccatgag tgtgctagca tcaatcgcat ttatggattc tatgatgaat 481 gcaaacgaag atttaatatt aaattgtgga agaccttcac tgattgtttt aactgtctgc 541 ctatagcagc cattgtggat gagaagatct tctgttgtca tggaggattg tcaccagacc 601 tgcaatctat ggagcagatt cggagaatta tgagacctac tgatgtccct gatacaggtt 661 tgctctgtga tttgctatgg tctgatccag ataaggatgt gcaaggctgg ggagaaaatg 721 atcgtggtgt ttcctttact tttGGAGCTG ATGTAGTCAG TAAATTTCTG AATCGTCATG 781 ATTTAGATTT GATTTGTCGA GCTCATCAGG TGGTGGAAGA TGGATATGAA TTTTTTGCTA 841 AACGACAGTT GGTAACCTTA TTTTCAGCCC CAAATTACTG TGGCGAGTTT GATAATGCTG 901 GTGGAATGAT GAGTGTGGAT GAAACTTTGA TGTGTTCATT TCAGATATTG AAACCATCTG 961 AAAAGAAAGC TAAATACCAG TATGGTGGAC TGAATTCTGG ACGTCCTGTC ACTCCACCTC 1021 GAACAGCTAA TCCGCGAAGA AAAGGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 1081 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 1141 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCCCTAACT 1201 TTAGAAGTTC CTTTCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 1261 agatt