Construct: ORF TRCN0000469405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018185.1_s317c1
- Derived from:
- ccsbBroadEn_08442
- DNA Barcode:
- CTCCTATAGCGATTTTCTCCCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSM5 (54988)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469405
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54988 | ACSM5 | acyl-CoA synthetase medium ... | NM_001324371.2 | 99.9% | 99.8% | 1505T>C |
| 2 | human | 54988 | ACSM5 | acyl-CoA synthetase medium ... | NM_017888.3 | 99.9% | 99.8% | 1505T>C |
| 3 | human | 54988 | ACSM5 | acyl-CoA synthetase medium ... | NM_001324372.2 | 97.6% | 92.9% | 1505T>C;1654_1655insAGGTGGCCTTTGTTTC;1722_1746del |
| 4 | human | 54988 | ACSM5 | acyl-CoA synthetase medium ... | NM_001324373.2 | 35.9% | 35.9% | 624_625ins1113 |
| 5 | mouse | 272428 | Acsm5 | acyl-CoA synthetase medium-... | NM_178758.3 | 84.1% | 86% | (many diffs) |
| 6 | mouse | 272428 | Acsm5 | acyl-CoA synthetase medium-... | XM_006507904.2 | 82.2% | 84.1% | (many diffs) |
| 7 | mouse | 272428 | Acsm5 | acyl-CoA synthetase medium-... | XM_006507905.2 | 48.9% | 50.4% | (many diffs) |
| 8 | mouse | 272428 | Acsm5 | acyl-CoA synthetase medium-... | XM_011241825.2 | 48.9% | 50.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1803
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag accatggctg agacacctag tcctccaggc actgaggaac tccagggcat 121 tctgtgggtc tcatgggaag ccagcacctc tacctgttcc tcagaagatc gtggccacct 181 gggaagccat cagcctggga aggcagctgg tgcctgagta cttcaacttc gcccatgatg 241 tgctggatgt gtggagtcgg ctggaagagg ctggacaccg ccccccaaat cctgccttct 301 ggtgggtcaa tggcacagga gcagagatca agtggagctt tgaggagctg gggaagcagt 361 ccaggaaggc agccaatgtg ctggggggtg catgcggcct gcagcctggg gacagaatga 421 tgctggtact cccacggctc ccggagtggt ggctggtcag tgtggcttgc atgcggacag 481 ggactgtgat gattccgggt gtgactcagc tgacagagaa ggacctcaag taccggctgc 541 aggcgtccag ggccaagtcc attatcacca gtgactccct agctccaagg gtggatgcca 601 tcagtgccga atgcccctcc ctccagacca agctgctggt gtcagacagc agtcggccag 661 gctggttgaa cttcagggaa ctcctccggg aggcttctac agagcacaac tgcatgagga 721 caaagagtcg agacccgctg gccatctact ttaccagcgg aaccaccggg gcccccaaga 781 tggtcgagca ctcccagagc agctacggac tgggttttgt ggccagcgga agacggtggg 841 tggccttgac cgaatctgac atcttctgga acacgactga cactggctgg gtgaaggcag 901 cctggactct cttctctgcc tggcctaatg gatcttgcat ttttgtgcat gagctgcccc 961 gagttgatgc caaagttatc ctgaatactc tctccaaatt cccgataacc accctctgct 1021 gtgtcccaac catctttcgg ctgcttgtgc aggaggatct gaccaggtac cagtttcaga 1081 gcctgaggca ctgtctgacc ggaggagagg ccctcaaccc tgacgtgagg gagaagtgga 1141 aacaccagac tggtgtggag ctgtacgaag gctatggcca gtctgaaacg gttgtcatct 1201 gtgccaatcc aaaaggcatg aaaatcaagt ctggatccat ggggaaggcg tccccaccct 1261 acgatgtgca gattgtggat gatgagggca acgtcctgcc tcctggagaa gaggggaatg 1321 ttgccgtccg tatcagaccc actcggccct tctgtttctt caattgctat ttggacaatc 1381 ctgagaagac agctgcatca gaacaagggg acttttacaT CACAGGGGAC CGAGCTCGCA 1441 TGGACAAGGA TGGCTACTTT TGGTTCATGG GAAGAAACGA CGATGTGATC AATTCTTCAA 1501 GCTACCGGAT CGGGCCTGTT GAAGTGGAAA GTGCCCTGGC AGAGCATCCT GCTGTCCTGG 1561 AGTCGGCTGC GGTCAGCAGC CCAGACCCCA TCAGGGGAGA GGTGGTAAAG GCATTTATAG 1621 TCCTTACTCC AGCCTACTCC TCTCATGACC CAGAGGCACT AACGCGGGAA CTCCAGGAGC 1681 ATGTGAAAAG GGTGACTGCT CCATACAAAT ACCCCAGGAA GGTGGCCTTT GTTTCAGAAC 1741 TGCCAAAGAC GGTTTCTGGA AAGATCCAAA GGAGTAAATT GCGAAGTCAG GAGTGGGGGA 1801 AATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1861 TCGGTCTCGA TTCTACGTAG TAATGAACTA GCCCGTAACT TGAAAGTATT TCGATTTCTT 1921 GGCTTTATAT ATCTTGTGGA AAGGACGACT CCTATAGCGA TTTTCTCCCC GCACGCGTTA 1981 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt