Construct: ORF TRCN0000469461
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018935.3_s317c1
- Derived from:
- ccsbBroadEn_15322
- DNA Barcode:
- TCCCAATGAACGACCGTCGGTTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP3K15 (389840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469461
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389840 | MAP3K15 | mitogen-activated protein k... | XM_011545511.2 | 98.9% | 1.2% | 0_1ins22;1598delC |
| 2 | human | 389840 | MAP3K15 | mitogen-activated protein k... | XM_011545510.2 | 86.6% | 1% | 1_347del;1967delC |
| 3 | human | 389840 | MAP3K15 | mitogen-activated protein k... | XM_011545507.3 | 63% | .7% | 1_1328del;2948delC |
| 4 | human | 389840 | MAP3K15 | mitogen-activated protein k... | XM_011545508.3 | 60.6% | .7% | 1_1328del;2425_2426ins87;2861delC |
| 5 | human | 389840 | MAP3K15 | mitogen-activated protein k... | NM_001001671.3 | 57.5% | .6% | 1_1673del;3293delC |
| 6 | mouse | 270672 | Map3k15 | mitogen-activated protein k... | NM_001163085.2 | 48.3% | .5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 156
- ORF length:
- 87
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcatgtctc acccacagaa atgaaacaga tgcacgaatg gaattttaca 121 gcctcttcca taaagggaat aagcctatca aagtttgatg aaaggtgttg ttttctttat 181 gtccatgata attctgatga ctttcaaatc tacttttcca ccgaagagca gtgcagtaga 241 tttttctctt tggtcaaaga gatgataacc aatacagcag gcagtacggt ggagctggag 301 ggagagaccg atggagacac cttggagtat gagtatgacc atgatgcaaa tggtgagaga 361 gttgtcttgg ggaaaggcac gtatgggatt gtgtatgctg gccgagatct gagcaatcaa 421 gtgcgaatag ccatcaaaga aatcccggag agagatagca ggtattctca gcctctgcac 481 gaggagatag ccctgcacaa gtaccttaag caccgcaata tcgttcagta cctgggctct 541 gtttcagaga acggctacat taagatattt atggagcagg tgcctggagg aagcctttct 601 gctcttctgc gatccaaatg ggggccgatg aaggaaccga caatcaagtt ttacaccaaa 661 cagatcctgg agggccttaa gtatcttcat gaaaaccaga tcgtgcacag agacataaag 721 ggcgataatg ttctggtgaa cacctacagc ggagtggtga aaatctccga ttttggaacc 781 tcgaaacgtc ttgcgggtgt gaacccctgc acagagactt ttactggcac cctgcagtac 841 atggcacctg agataattga ccaagggcct cgcggatatg gtgccccagc cgatatctgg 901 tccctgggct gcaccatcat tgagatggcc accagcaagc ctccgttcca tgagcttggt 961 gagccgcagg cagccatgtt caaagtgggc atgtttaaga tccaccctga gattccagaa 1021 gccctttcag ctgaagcccg agccttcatt ttatcctgtt tcgagcctga cccccacaaa 1081 cgtgccacca ctgctgagct actgagagag ggtttcttaa ggcaggtgaa caagggcaag 1141 aagaaccgaa ttgccttcaa gccctcagaa ggtccccgcg gtgtcgtcct ggccctgccc 1201 acacagggag agcccatggc caccagcagc agcgagcacg gctctgtctc cccagactcc 1261 gacgcccagc ctgacgcact ctttgagagg acccgggcgc ccaggcacca ccttggccac 1321 ctcctcagtg ttccagacga gagctcagcc ttggaagacc ggggcttggc ctcgtccccg 1381 gaggacaggg accagggcct cttcctgcta cgcaaggaca gtgagcgccg tgccatcctg 1441 tacaaaatcc tctgggagga gcagaaccag gtggcttcca acctgcagga gtgtgtggcc 1501 cagagttccg aagagttgca tctctcagtt ggacacatca agcaaatcat tgggatcctg 1561 agggacttca tccgctcccc agagcaccgg gtgatggcga ccacaatatc aaagctcaag 1621 gtggacctgg actttgacag ctcgtccatc agtcagattc acctggtgct gttcggattt 1681 caggatgcgt aaataaaatt ttgaggaacc acttaattag gccccactgg atgttcgcga 1741 tggacaacat catccgccga gcggtgcagg ccgcggtcac cattctcatc ccagagctcc 1801 gagcccactt tgagcctacc tgtgagactg aaggggtaga taaggacatg gatgaagcgg 1861 aagagggcta tcccccagcc accggacctg gccaggaggc ccagccccac cagcagcacc 1921 tgagcctcca gctgggtgag ctcagacagg agaccaacag acttttggaa cacctagttG 1981 AAAAAGAGAG AGAGTACCAG AATCTTCTGC GGCAAACTCT AGAACAGAAA ACTCAAGAAT 2041 TGTATCACCT TCAGTTAAAA TTAAAATCGA ATTGTATTAC AGAGAACCCA GCAGGCCCCT 2101 ACGGGCAGAG AACAGATAAA GAGCTTATAG ACTGGTTGCG GCTGCAAGGA GCTGATGCAA 2161 AGACAATTGA AAAGATTGTT GAAGAGGGTT ATACACTTTC GGATATTCTT AATGAGATCA 2221 CTAAGGAAGA TCTAAGATAC CTTCGACTAC GGGGTGGTCT CCTCTGCAGA CTCTGGAGTG 2281 CGGTCTCCCA GTACAGAAGG GCTCAGGAGG CCTCAGAAAC CAAAGACAAG GCTTTGCCAA 2341 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2401 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2461 TATCTTGTGG AAAGGACGAT CCCAATGAAC GACCGTCGGT TCCACGCGTT AAGTCgacaa 2521 tcaacctctg gattacaaaa tttgtgaaag att