Construct: ORF TRCN0000469475
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004616.1_s317c1
- Derived from:
- ccsbBroadEn_03299
- DNA Barcode:
- GGTGCAGACTGCTTGTCACCTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR83OS (51398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469475
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51398 | WDR83OS | WD repeat domain 83 opposit... | NM_016145.4 | 100% | 100% | |
| 2 | human | 51398 | WDR83OS | WD repeat domain 83 opposit... | XM_017026864.1 | 100% | 100% | |
| 3 | mouse | 414077 | Wdr83os | WD repeat domain 83 opposit... | NM_001001493.2 | 91.8% | 100% | (many diffs) |
| 4 | mouse | 100040052 | Gm2573 | predicted gene 2573 | XM_001474178.5 | 90.5% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 68
- ORF end:
- 386
- ORF length:
- 318
- Sequence:
-
1 TTTCTTCCAT TTCAGGTGTC GTGAGGCTAG CATCGATTGA TCAACAAGTT TGTACAAAAA 61 AGTTGGCATG TCCACTAACA ATATGTCGGA CCCACGGAGG CCGAACAAAG TGCTGAGGTA 121 CAAGCCCCCG CCGAGCGAAT GTAACCCGGC CTTGGACGAC CCGACGCCGG ACTACATGAA 181 CCTGCTGGGC ATGATCTTCA GCATGTGCGG CCTCATGCTT AAGCTGAAGT GGTGTGCTTG 241 GGTCGCTGTC TACTGCTCCT TCATCAGCTT TGCCAACTCT CGGAGCTCGG AGGACACGAA 301 GCAAATGATG AGTAGCTTCA TGCTGTCCAT CTCTGCCGTG GTGATGTCCT ATCTGCAGAA 361 TCCTCAGCCC ATGACGCCCC CATGGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 421 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 481 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGTGCAGAC 541 TGCTTGTCAC CTAATACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 601 agatt