Construct: ORF TRCN0000469481
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015578.3_s317c1
- Derived from:
- ccsbBroadEn_00208
- DNA Barcode:
- ATCTGGCTTATGCACTTAGGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALM3 (808)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469481
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 808 | CALM3 | calmodulin 3 | NM_001329922.1 | 100% | 100% | |
| 2 | human | 808 | CALM3 | calmodulin 3 | NM_005184.4 | 100% | 100% | |
| 3 | human | 801 | CALM1 | calmodulin 1 | NM_006888.6 | 81.8% | 100% | (many diffs) |
| 4 | human | 805 | CALM2 | calmodulin 2 | NM_001743.6 | 81.6% | 100% | (many diffs) |
| 5 | human | 801 | CALM1 | calmodulin 1 | NM_001363670.1 | 81.3% | 98.6% | (many diffs) |
| 6 | human | 808 | CALM3 | calmodulin 3 | NM_001329921.1 | 75.8% | 75.8% | 0_1ins108 |
| 7 | human | 808 | CALM3 | calmodulin 3 | NM_001329923.1 | 75.8% | 75.8% | 0_1ins108 |
| 8 | human | 808 | CALM3 | calmodulin 3 | NM_001329924.2 | 75.8% | 75.8% | 0_1ins108 |
| 9 | human | 808 | CALM3 | calmodulin 3 | NM_001329925.2 | 75.8% | 75.8% | 0_1ins108 |
| 10 | human | 808 | CALM3 | calmodulin 3 | NM_001329926.2 | 75.8% | 75.8% | 0_1ins108 |
| 11 | human | 801 | CALM1 | calmodulin 1 | NM_001363669.1 | 62.1% | 75.8% | (many diffs) |
| 12 | human | 805 | CALM2 | calmodulin 2 | NM_001305625.1 | 61.7% | 75.8% | (many diffs) |
| 13 | human | 805 | CALM2 | calmodulin 2 | NM_001305626.1 | 61.7% | 75.8% | (many diffs) |
| 14 | human | 805 | CALM2 | calmodulin 2 | NM_001305624.1 | 61.5% | 75.1% | (many diffs) |
| 15 | mouse | 12315 | Calm3 | calmodulin 3 | NM_007590.3 | 92.6% | 100% | (many diffs) |
| 16 | mouse | 12314 | Calm2 | calmodulin 2 | NM_007589.5 | 83.6% | 100% | (many diffs) |
| 17 | mouse | 12313 | Calm1 | calmodulin 1 | NM_009790.5 | 83.2% | 100% | (many diffs) |
| 18 | mouse | 70405 | Calml3 | calmodulin-like 3 | NM_027416.3 | 80.3% | 87.2% | (many diffs) |
| 19 | mouse | 12314 | Calm2 | calmodulin 2 | NM_001355703.1 | 62.8% | 75.8% | (many diffs) |
| 20 | mouse | 12313 | Calm1 | calmodulin 1 | NM_001313934.1 | 62.1% | 75.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 513
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaccagctg actgaggagc agattgcaga gttcaaggag gccttctccc 121 tctttgacaa ggatggagat ggcactatca ccaccaagga gttggggaca gtgatgagat 181 cccTGGGACA GAACCCCACT GAAGCAGAGC TGCAGGATAT GATCAATGAG GTGGATGCAG 241 ATGGGAACGG GACCATTGAC TTCCCGGAGT TCCTGACCAT GATGGCCAGA AAGATGAAGG 301 ACACAGACAG TGAGGAGGAG ATCCGAGAGG CGTTCCGTGT CTTTGACAAG GATGGGAATG 361 GCTACATCAG CGCCGCAGAG CTGCGTCACG TAATGACGAA CCTGGGGGAG AAGCTGACCG 421 ATGAGGAGGT GGATGAGATG ATCAGGGAGG CTGACATCGA TGGAGATGGC CAGGTCAATT 481 ATGAAGAGTT TGTACAGATG ATGACTGCAA AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 661 CTGGCTTATG CACTTAGGCC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt