Construct: ORF TRCN0000469486
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016821.1_s317c1
- Derived from:
- ccsbBroadEn_01374
- DNA Barcode:
- TGTTACCAGGTTATACAGAGCGCG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAP1A (5906)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469486
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5906 | RAP1A | RAP1A, member of RAS oncoge... | NM_001010935.2 | 99.8% | 97.3% | 542delG |
| 2 | human | 5906 | RAP1A | RAP1A, member of RAS oncoge... | NM_001291896.1 | 99.8% | 97.3% | 542delG |
| 3 | human | 5906 | RAP1A | RAP1A, member of RAS oncoge... | NM_001370216.1 | 99.8% | 97.3% | 542delG |
| 4 | human | 5906 | RAP1A | RAP1A, member of RAS oncoge... | NM_001370217.1 | 99.8% | 97.3% | 542delG |
| 5 | human | 5906 | RAP1A | RAP1A, member of RAS oncoge... | NM_002884.3 | 99.8% | 97.3% | 542delG |
| 6 | human | 5908 | RAP1B | RAP1B, member of RAS oncoge... | NM_001010942.3 | 82.3% | 93% | (many diffs) |
| 7 | human | 5908 | RAP1B | RAP1B, member of RAS oncoge... | NM_015646.6 | 82.3% | 93% | (many diffs) |
| 8 | human | 5908 | RAP1B | RAP1B, member of RAS oncoge... | NM_001251921.2 | 73.3% | 83.3% | (many diffs) |
| 9 | human | 5908 | RAP1B | RAP1B, member of RAS oncoge... | NM_001251917.2 | 62.7% | 70.9% | (many diffs) |
| 10 | human | 5908 | RAP1B | RAP1B, member of RAS oncoge... | NM_001251918.2 | 62.7% | 70.9% | (many diffs) |
| 11 | human | 5908 | RAP1B | RAP1B, member of RAS oncoge... | NM_001251922.2 | 61.2% | 68.2% | (many diffs) |
| 12 | mouse | 109905 | Rap1a | RAS-related protein 1a | NM_145541.5 | 94% | 97.3% | (many diffs) |
| 13 | mouse | 109905 | Rap1a | RAS-related protein 1a | XM_006500907.4 | 94% | 97.3% | (many diffs) |
| 14 | mouse | 109905 | Rap1a | RAS-related protein 1a | XM_030252384.1 | 94% | 97.3% | (many diffs) |
| 15 | mouse | 109905 | Rap1a | RAS-related protein 1a | XM_030252385.1 | 94% | 97.3% | (many diffs) |
| 16 | mouse | 109905 | Rap1a | RAS-related protein 1a | XM_030252386.1 | 94% | 97.3% | (many diffs) |
| 17 | mouse | 215449 | Rap1b | RAS related protein 1b | NM_024457.2 | 81.5% | 93% | (many diffs) |
| 18 | mouse | 109905 | Rap1a | RAS-related protein 1a | XR_001783642.2 | 20% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg tgagtacaag ctagtggtcc ttggttcagg aggcgttggg aagtctgctc 121 tgacagttca gtttgttcag ggaatttttg ttgaaaaata tgacccaacg atagaagatT 181 CCTACAGAAA GCAAGTTGAA GTCGATTGCC AACAGTGTAT GCTCGAAATC CTGGATACTG 241 CAGGGACAGA GCAATTTACA GCAATGAGGG ATTTGTATAT GAAGAACGGC CAAGGTTTTG 301 CACTAGTATA TTCTATTACA GCTCAGTCCA CGTTTAACGA CTTACAGGAC CTGAGGGAAC 361 AGATTTTACG GGTTAAGGAC ACGGAAGATG TTCCAATGAT TTTGGTTGGC AATAAATGTG 421 ACCTGGAAGA TGAGCGAGTA GTTGGCAAAG AGCAGGGCCA GAATTTAGCA AGACAGTGGT 481 GTAACTGTGC CTTTTTAGAA TCTTCTGCAA AGTCAAAGAT CAATGTTAAT GAGATATTTT 541 ATGACCTGGT CAGACAGATA AATAGGAAAA CACCAGTGGA AAAGAAGAAG CCTAAAAAGA 601 AATCATTCTG CTGCTCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 661 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 721 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGTTACCA GGTTATACAG 781 AGCGCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt