Construct: ORF TRCN0000469488
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014362.1_s317c1
- Derived from:
- ccsbBroadEn_02852
- DNA Barcode:
- ATCCGTGACTGCGCTGCTGCGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAD54B (25788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469488
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25788 | RAD54B | RAD54 homolog B | NM_012415.3 | 100% | 100% | |
| 2 | human | 25788 | RAD54B | RAD54 homolog B | NM_001205263.2 | 79.7% | 79.7% | 0_1ins552 |
| 3 | human | 25788 | RAD54B | RAD54 homolog B | NM_001205262.3 | 12.3% | 11.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2796
- ORF length:
- 2730
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag acgatctgca gcaccaagtc agttgcaggg gaattccttc aaaaaaccaa 121 aatttatacc tccaggaaga agtaatccag gtctgaatga agagattaca aaactgaatc 181 cagatataaa attatttgag ggtgttgcaa ttaataacac ctttctcccg tcacaaaatg 241 atcttagaat atgcagttta aatctgccta gtgaagaaag tactagagaa atcaataaca 301 gagataattg cagtggaaaa tattgttttg aagcacctac actggcaaca ttagatccac 361 ctcatacagt tcattcggct cctaaagaag tagcagtgtc caaggaacaa gaagagaaat 421 ctgatagcct agttaaatat ttcagtgttg tttggtgtaa gccttcaaag aaaaaacata 481 aaaagtggga aggtgatgct gttcttattg taaaaggaaa gtcatttata ttaaagaatt 541 tggaaggcaa agacattgga agaggcattg gttataaatt caaagagctt gaaaagattg 601 aagagggcca aacactgatg atttgtggaa aagaaataga agtcatgggt gtaatctctc 661 cagatgactt cagcagtggc aggtgttttc agcttggagg aggaagtact gctatctcgc 721 attcttctca ggttgccagg aaatgtttct ctaacccttt caaaagtgtt tgtaaaccaa 781 gttcaaagga aaatagacag aatgatttcc aaaattgcaa accacgccat gacccatata 841 cgccaaattc cctcgttatg ccacgaccag ataagaatca ccagtgggta ttcaataaga 901 actgtttccc tcttgtggat gtagtgattg atccttacct tgtatatcat cttcgaccac 961 atcagaaaga aggaatcata ttcctttatg aatgtgtaat gggaatgaga atgaatggca 1021 gatgtggagc tattcttgct gatgaaatgg gtttagggaa gacattgcaa tgtatttcgc 1081 tcatctggac cctgcagtgt cagggaccct atggaggcaa gccagtaata aagaagacac 1141 taattgtcac acctggaagc ttggtgaata attggaagaa agaatttcaa aaatggctag 1201 gaagtgaaag gatcaagata tttactgttg atcaggacca caaagttgaa gaattcatca 1261 agtctatatt ttattctgtt cttattatca gttatgaaat gttacttcgt tccctggatc 1321 aaattaagaa tataaaattt gatcttctaa tctgtgacga ggggcatcgt ttgaagaaca 1381 gtgccattaa gacaactaca gccctcatta gcctttcttg tgagaaaaga ataattctaa 1441 ctggtactcc aattcagaat gatctgcaag aattttttgc attaattgat tttgtaaatc 1501 caggaatatt aggctctttg tcatcttata ggaaaatata tgaagaaccc atcattttat 1561 cgagagaacc ttctgcttct gaggaagaaa aggagttagg agaaagaaga gcagctgaac 1621 ttacttgcct cactggactc tttatcctta gaagaaccca agaaattata aataaatatc 1681 tcccacctaa aatagagaat gttgtctttt gccgaccagg agcactacag attgagcttt 1741 atcgaaagct gttaaattct caggttgtca ggttctgcct tcaagggttg ttggaaaata 1801 gtccccatct aatatgtata ggagctctta aaaaactgtg caatcacccc tgccttttgt 1861 tcaactctat aaaggaaaag gaatgtagct caacttgtga taaaaatgaa gaaaagagtc 1921 tatacaaagg cttgctaagt gtgtttcctg ctgactacaa ccctctcctg tttactgaaa 1981 aggagtcagg aaaactacag gtgttgtcca agctcttagc ggttatccac gaacttcgac 2041 ctactgaaaa ggtggtgttg gtatccaact atacacaaac cttgaacatt ttacaagaag 2101 tatgtaagcg tcatggatat gcttatacaa gacttgatgg acaaacacca atctctcaaa 2161 ggcagcagat tgttgatggc tttaacagtc aacactcttc tttttttatt tttttgttaa 2221 gttcaaaagc tggtggtgta ggacttaacc tcattggagg atctcactta attctctatg 2281 acattgattg gaatccagcc actgacattc aggcaatgtc tagagtatgg agagatggTC 2341 AGAAATATCC TGTACATATT TACAGACTCC TAACTACAGG TACAATAGAA GAAAAGATCT 2401 ATCAAAGGCA GATCAGTAAG CAAGGTCTTT GTGGGGCAGT TGTCGACCTC ACCAAGACAT 2461 CTGAACATAT TCAGTTTTCA GTAGAAGAAC TTAAAAATTT GTTCACATTA CATGAAAGTT 2521 CAGATTGTGT TACTCATGAT CTGCTTGACT GTGAGTGTAC AGGAGAAGAA GTTCATACAG 2581 GTGATTCGTT GGAAAAATTC ATTGTCTCTA GAGATTGTCA GCTTGGTCCA CATCACCAGA 2641 AATCTAACTC CCTGAAACCT CTTTCTATGT CCCAGCTGAA GCAATGGAAA CATTTTTCTG 2701 GAGATCATTT AAATCTTACA GATCCTTTTC TTGAAAGAAT AACAGAAAAT GTGTCATTCA 2761 TTTTTCAGAA TATAACCACT CAAGCTACTG GCACATACCC AACTTTCTTG TACAAAGTGG 2821 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2881 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2941 AATCCGTGAC TGCGCTGCTG CGTGGACGCG TTAAGTCgac aatcaacctc tggattacaa 3001 aatttgtgaa agatt