Construct: ORF TRCN0000469516
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018191.1_s317c1
- Derived from:
- ccsbBroadEn_10227
- DNA Barcode:
- ACTCCATTCTCACCCTCTGCATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF85 (7639)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469516
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_001256172.2 | 63.2% | 63.2% | 1_96del |
| 2 | human | 440519 | ZNF724 | zinc finger protein 724 | XM_024451521.1 | 48.2% | 43.6% | (many diffs) |
| 3 | human | 9534 | ZNF254 | zinc finger protein 254 | NM_001278665.2 | 41.9% | 36.4% | (many diffs) |
| 4 | human | 340252 | ZNF680 | zinc finger protein 680 | XM_017012129.1 | 35.8% | 31.2% | (many diffs) |
| 5 | human | 7697 | ZNF138 | zinc finger protein 138 | NM_001271637.2 | 31.4% | 17.2% | (many diffs) |
| 6 | human | 7639 | ZNF85 | zinc finger protein 85 | NR_045830.2 | 10.5% | 1_259del;425_1568del | |
| 7 | human | 113835 | ZNF257 | zinc finger protein 257 | NM_001316996.2 | 8.6% | 7.3% | (many diffs) |
| 8 | human | 113835 | ZNF257 | zinc finger protein 257 | NM_033468.4 | 8.1% | 6.9% | (many diffs) |
| 9 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_001256171.1 | 7.9% | 7.3% | (many diffs) |
| 10 | human | 7639 | ZNF85 | zinc finger protein 85 | XM_011528263.2 | 7.9% | 7.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 142
- ORF end:
- 307
- ORF length:
- 165
- Sequence:
-
1 TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG 61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA 121 AGTTTGTACA AAAAAGTTGG CATGTTAGAG AACTACAGAA ACCTGGTCTT CCTGGGTATT 181 ACTGTTTCTA AGCCAGACCT GATCACTTGT CTGGAGCAAG GGAAAGAGGC CTGGAGTATG 241 AAGAGACATG AGATCATGGT GGCCAAACCC ACAGAATCTT ACTCTGTCAC CCAGTCTGGA 301 ATGCAGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT 361 CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT 421 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACTCCATT CTCACCCTCT GCATTCACGC 481 GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt