Transcript: Human NM_001316996.2

Homo sapiens zinc finger protein 257 (ZNF257), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
ZNF257 (113835)
Length:
4000
CDS:
367..1962

Additional Resources:

NCBI RefSeq record:
NM_001316996.2
NBCI Gene record:
ZNF257 (113835)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001316996.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000018037 CTCATACCTTACCGTACATAA pLKO.1 1851 CDS 100% 13.200 9.240 N ZNF257 n/a
2 TRCN0000412731 TTTGCCCAGAGCGAGACATAA pLKO_005 524 CDS 100% 13.200 9.240 N ZNF257 n/a
3 TRCN0000433690 CTCAACATTTACTAAGCATAA pLKO_005 2096 3UTR 100% 10.800 7.560 N ZNF257 n/a
4 TRCN0000414562 GCCCTTCAAATATGATGAATG pLKO_005 1119 CDS 100% 10.800 7.560 N ZNF257 n/a
5 TRCN0000421339 GCGAAACTACAATCCTGAATG pLKO_005 2376 3UTR 100% 10.800 7.560 N ZNF257 n/a
6 TRCN0000018035 CGGTCTTCACACATTACTCAA pLKO.1 1072 CDS 100% 4.950 3.465 N ZNF257 n/a
7 TRCN0000018033 CCTCAGCTCTTACTACCCTTA pLKO.1 1160 CDS 100% 4.050 2.835 N ZNF257 n/a
8 TRCN0000416136 AGTGCGAAACTCTAGAAATAT pLKO_005 2456 3UTR 100% 15.000 9.000 N ZNF257 n/a
9 TRCN0000018036 CCCAGTTATGTGTTCTCATAT pLKO.1 492 CDS 100% 13.200 6.600 Y ZNF257 n/a
10 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 941 CDS 100% 13.200 6.600 Y Zfp934 n/a
11 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 941 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
12 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 941 CDS 100% 13.200 6.600 Y EG668616 n/a
13 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 3794 3UTR 100% 4.950 2.475 Y ORAI2 n/a
14 TRCN0000016587 CCTGGGTATTGCTGTCTCTAA pLKO.1 396 CDS 100% 4.950 2.475 Y ZNF675 n/a
15 TRCN0000074123 CGCCTGTAATCCCAACACTTT pLKO.1 3716 3UTR 100% 4.950 2.475 Y GJD4 n/a
16 TRCN0000166650 CGCCTGTAATCCCAACACTTT pLKO.1 3716 3UTR 100% 4.950 2.475 Y C9orf85 n/a
17 TRCN0000018502 GATGTTAGAGAACTACAGAAA pLKO.1 366 5UTR 100% 4.950 2.475 Y ZNF493 n/a
18 TRCN0000149073 GCAAAGCCTTTAACCAGTCTT pLKO.1 1664 CDS 100% 4.950 2.475 Y ZNF714 n/a
19 TRCN0000107758 TGTCTCTAAGCCAGACCTGAT pLKO.1 408 CDS 100% 4.050 2.025 Y ZNF273 n/a
20 TRCN0000147126 CCCAGTTATGTGTTCTCATTT pLKO.1 492 CDS 100% 13.200 6.600 Y ZNF43 n/a
21 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 3791 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001316996.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13028 pDONR223 100% 99.8% 99.8% None 706C>A;967C>G n/a
2 ccsbBroad304_13028 pLX_304 0% 99.8% 99.8% V5 706C>A;967C>G n/a
3 TRCN0000468257 GTACACCAGACCACTACATGCGAC pLX_317 25.6% 99.8% 99.8% V5 706C>A;967C>G n/a
4 ccsbBroadEn_15278 pDONR223 59.5% 75.7% 64.5% None (many diffs) n/a
5 ccsbBroad304_15278 pLX_304 0% 75.7% 64.5% V5 (many diffs) n/a
6 ccsbBroadEn_15167 pDONR223 53.6% 72.7% 28.2% None (many diffs) n/a
7 ccsbBroad304_15167 pLX_304 0% 72.7% 28.2% V5 (not translated due to prior stop codon) (many diffs) n/a
8 ccsbBroadEn_15273 pDONR223 50.9% 68.5% 58.7% None (many diffs) n/a
9 ccsbBroad304_15273 pLX_304 0% 68.5% 58.7% V5 (many diffs) n/a
10 TRCN0000472761 GCGGTTCAATGTTGTAGTCTTGTG pLX_317 63.8% 28.2% 23.8% V5 (not translated due to frame shift) (many diffs) n/a
11 ccsbBroadEn_11549 pDONR223 100% 8.7% 8% None (many diffs) n/a
12 ccsbBroad304_11549 pLX_304 94.6% 8.7% 8% V5 (many diffs) n/a
13 TRCN0000468281 AACATTAGGAAAGAACCCCCACCC pLX_317 100% 8.7% 8% V5 (many diffs) n/a
14 ccsbBroadEn_10227 pDONR223 100% 8.6% 7.3% None (many diffs) n/a
15 ccsbBroad304_10227 pLX_304 0% 8.6% 7.3% V5 (many diffs) n/a
16 TRCN0000469516 ACTCCATTCTCACCCTCTGCATTC pLX_317 100% 8.6% 7.3% V5 (many diffs) n/a
17 ccsbBroadEn_15729 pDONR223 0% 8.2% 7.6% None (many diffs) n/a
18 ccsbBroad304_15729 pLX_304 0% 8.2% 7.6% V5 (many diffs) n/a
19 TRCN0000470492 GCCGACTTGCTCCATGATGCAGCT pLX_317 100% 8.2% 7.6% V5 (many diffs) n/a
20 ccsbBroadEn_11384 pDONR223 100% 7.8% 6.8% None (many diffs) n/a
21 ccsbBroad304_11384 pLX_304 0% 7.8% 6.8% V5 (many diffs) n/a
22 TRCN0000470576 TACATACAGACCTACACGTAGACC pLX_317 100% 7.8% 6.8% V5 (many diffs) n/a
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