Construct: ORF TRCN0000469570

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017700.1_s317c1
Derived from:
ccsbBroadEn_12567
DNA Barcode:
GAGATGCATTAAATCCTGTACTAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WDR25 (79446)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469570
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79446 WDR25 WD repeat domain 25 NM_001350950.1 94.9% 94.1% (many diffs)
2 human 79446 WDR25 WD repeat domain 25 XM_005268057.3 94.6% 93.1% (many diffs)
3 human 79446 WDR25 WD repeat domain 25 NM_001350949.1 91.9% 88.8% (many diffs)
4 human 79446 WDR25 WD repeat domain 25 XM_011537151.3 90.3% 86.9% (many diffs)
5 human 79446 WDR25 WD repeat domain 25 NM_001161476.2 51.8% 50.1% (many diffs)
6 human 79446 WDR25 WD repeat domain 25 NM_001350947.2 51.8% 50.1% (many diffs)
7 human 79446 WDR25 WD repeat domain 25 NM_001350948.1 51.8% 50.1% (many diffs)
8 human 79446 WDR25 WD repeat domain 25 NM_024515.6 51.8% 50.1% (many diffs)
9 mouse 212198 Wdr25 WD repeat domain 25 NM_177602.3 46.6% 47.2% (many diffs)
10 mouse 212198 Wdr25 WD repeat domain 25 XM_006515657.2 46.6% 47.2% (many diffs)
11 mouse 212198 Wdr25 WD repeat domain 25 XM_006515658.3 46.6% 47.2% (many diffs)
12 mouse 212198 Wdr25 WD repeat domain 25 XM_006515660.3 46.6% 47.2% (many diffs)
13 mouse 212198 Wdr25 WD repeat domain 25 XM_011244054.2 46.6% 47.2% (many diffs)
14 mouse 212198 Wdr25 WD repeat domain 25 XM_011244055.2 46.6% 47.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
927
ORF length:
861
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc agctctgtgc ctggtgctgt gctggtttca agggctgttg ggagaggtat
121ggaacgccgt ggactccggg cactgcctgc agacctactc cctgcacaca gaggcagtgc
181gggccgcccg gtgggctccc tgtggccggc gcatcctcag tggtggcttt gacttcgcgc
241tgcacctaac agaccttgaa acaggaaccc agctatttag tggtcgaagt gactttagaa
301tcactacctt gaaattccat ccaaaagacc acaacatctt tttatgtgga ggcttcagct
361ctgaaatgaa agcttgggat ataaggactg gcaaggtgat gagaagctac aaggcgacca
421tccagcagac cttggacatc ctgttcctcc gggaaggctc cgagttcctg agcagcacag
481acgcttccac ccgggactca gctgaccgca ccattattgc ctgggatttc cggacctctg
541ccaaaatctc caaccagatt ttccacgaga ggttcacctg ccccagcctc gccttgcacc
601cgagagagcc cgtgttcctg gcacagacca atggcaacta cctggccctt ttctccactg
661tgtggcccta ccggatgagc agacggcggc gctatgaagg gcacaaggtg gagggctact
721cagtgggctg cgagtgctcc cCAGGCGGTG ACTTGCTGGT GACGGGCAGC GCCGATGGCC
781GGGTCCTGAT GTACAGCTTC CGCACAGCCA GCCGAGCATG CACACTGCAG GGGCACACAC
841AGGCCTGTGT CGGCACCACC TACCACCCCG TGCTGCCCTC CGTCCTCGCC ACCTGCTCCT
901GGGGAGGGGA CATGAAGATC TGGCACTGTC TAACTTTCTT GTACAAAGTG GTTGATATCG
961GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1021AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAGATGCA
1081TTAAATCCTG TACTATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1141aagatt

Download FASTA (ORF) (Full)