Transcript: Mouse XM_011244055.2

PREDICTED: Mus musculus WD repeat domain 25 (Wdr25), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Wdr25 (212198)
Length:
4356
CDS:
1764..3371

Additional Resources:

NCBI RefSeq record:
XM_011244055.2
NBCI Gene record:
Wdr25 (212198)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011244055.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000442025 GCGCAAGCGTGTGTTAGTAAA pLKO_005 3481 3UTR 100% 13.200 18.480 N Wdr25 n/a
2 TRCN0000215900 CAAACCTTCTCATGATCAAAG pLKO.1 2171 CDS 100% 10.800 15.120 N Wdr25 n/a
3 TRCN0000433729 GGGTGACACGCAATCAGTAAG pLKO_005 3523 3UTR 100% 10.800 15.120 N Wdr25 n/a
4 TRCN0000189733 GAACCATTATCGCCTGGGATT pLKO.1 2950 CDS 100% 4.050 5.670 N Wdr25 n/a
5 TRCN0000190248 CGAGTTTCTTAGCAGTACGGA pLKO.1 2903 CDS 100% 0.750 1.050 N Wdr25 n/a
6 TRCN0000431365 GAGTTCATCCAGCCATATTTG pLKO_005 2391 CDS 100% 13.200 9.240 N Wdr25 n/a
7 TRCN0000436719 GGCTTCAGTGACTCGGCTATA pLKO_005 3436 3UTR 100% 10.800 7.560 N Wdr25 n/a
8 TRCN0000421886 TCAGTGGTGGCTTCGACTTTG pLKO_005 2659 CDS 100% 10.800 7.560 N Wdr25 n/a
9 TRCN0000200962 CCCACTATAAGACACCAACAA pLKO.1 3914 3UTR 100% 4.950 3.465 N Wdr25 n/a
10 TRCN0000189706 GCTCCGAGTTTCTTAGCAGTA pLKO.1 2899 CDS 100% 4.050 2.835 N Wdr25 n/a
11 TRCN0000202021 CCAGATCTTCCATGAGAGGTA pLKO.1 2996 CDS 100% 2.640 1.848 N Wdr25 n/a
12 TRCN0000202341 GAGACATCAAGATCTGGCACT pLKO.1 3349 CDS 100% 2.160 1.512 N Wdr25 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011244055.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12567 pDONR223 100% 46.6% 47.2% None (many diffs) n/a
2 ccsbBroad304_12567 pLX_304 0% 46.6% 47.2% V5 (many diffs) n/a
3 TRCN0000469570 GAGATGCATTAAATCCTGTACTAT pLX_317 22.2% 46.6% 47.2% V5 (many diffs) n/a
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