Construct: ORF TRCN0000469578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004484.1_s317c1
- Derived from:
- ccsbBroadEn_14331
- DNA Barcode:
- AGCAGCATCACTATAGTGAGTGCT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNK17 (89822)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89822 | KCNK17 | potassium two pore domain c... | NM_031460.4 | 99.6% | 98.7% | 8G>A;61A>G;992delA |
| 2 | human | 89822 | KCNK17 | potassium two pore domain c... | NM_001135111.2 | 75.4% | 69.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1062
- ORF length:
- 996
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta ccaaccgcga gcccgggcgg ctcccgaggg cagggtccgg ggctgcgcgg 121 tgcccggcac cgtgctcctg ctgctcgcct acctggctta cctggcgctg ggcaccggcg 181 tgttctggac gctggagggc cgcgcggcgc aggactccag ccgcagcttc cagcgcgaca 241 agtgggagct gttgcagaac ttcacgtgtc tggaccgccc ggcgctggac tcgctgatcc 301 gggatgtcgt ccaagcatac aaaaacggag ccagcctcct cagcaacacc accagcatgg 361 ggcgctggga gctcgtgggc tccttcttct tttctgtgtc caccatcacc accattggct 421 atggcaacct gagccccaac acgatggctg cccgcctctt ctgcatcttc tttgcccttg 481 tggggatccc actcaacctc gtggtgctca accgactggg gcatctcatg cagcagggag 541 taaaccactg ggccagcagg ctggggggca cctggcagga tcctgacaag gcgcggtggc 601 tggcgggctc tggcgccctc ctctcgggcc tcctgctctt cctgctgctg ccaccgctgc 661 tcttctccca catggagggc tggagctaca cagagggctt ctacttcgcc ttcatcaccc 721 tcagcaccgt gggcttcggc gactacgtga ttggaatgaa cccctcccag aggtacccac 781 tgtggtacaa gaacatggtg tcCCTGTGGA TCCTCTTTGG GATGGCATGG CTGGCCTTGA 841 TCATCAAACT CATCCTCTCC CAGCTGGAGA CGCCAGGGAG GGTATGTTCC TGCTGCCACC 901 ACAGCTCTAA GGAAGACTTC AAGTCCCAAA GCTGGAGACA GGGACCTGAC CGGGAGCCAG 961 AGTCCCACTC CCCACAGCAA GGATGCTATC CAGAGGGACC CATGGGAATC ATACAGCATC 1021 TGGAACCTTC TGCTCACGCT GCAGGCTGTG GCAAGGCAGC TACCCAACTT TCTTGTACAA 1081 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 1141 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 1201 GGACGAAGCA GCATCACTAT AGTGAGTGCT ACGCGTTAAG TCgacaatca acctctggat 1261 tacaaaattt gtgaaagatt