Construct: ORF TRCN0000469606
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009167.1_s317c1
- Derived from:
- ccsbBroadEn_14942
- DNA Barcode:
- AAGCACGTCTTTTGCCCATGGCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNA14 (9630)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469606
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9630 | GNA14 | G protein subunit alpha 14 | NM_004297.4 | 99.8% | 98.5% | 1052delA;1059delC |
| 2 | human | 9630 | GNA14 | G protein subunit alpha 14 | XM_017015341.1 | 84.6% | 83.3% | 0_1ins162;890delA;897delC |
| 3 | human | 9630 | GNA14 | G protein subunit alpha 14 | XM_011519224.1 | 66.5% | 65.3% | 0_1ins354;698delA;705delC |
| 4 | mouse | 14675 | Gna14 | guanine nucleotide binding ... | NM_008137.4 | 89.3% | 95.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1131
- ORF length:
- 1065
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggctgctgc tgcctgtccg cggaggagaa ggagtcgcag cgcatcagcg 121 cggagatcga gcgacagctt cgtcgggaca agaaggacgc gcgccgtgag cttaagctgc 181 tgctgctggg aactggtgaa agtgggaaaa gcacctttat caagcagatg agaattatcc 241 atgggtctgg ttacagcgac gaagacagaa aggggttcac gaagctggtt taccaaaaca 301 tattcaccgc catgcaagcc atgatcagag cgatggacac gctaaggata cagtatgtgt 361 gtgaacagaa taaggaaaat gcccagataa tcagagaagt ggaagtggac aaggtctcca 421 tgctctccag ggagcaggtg gaggccatca agcagctctg gcaagatcca ggcatccagg 481 agtgttacga caggaggagg gagtaccagc tgtcggactc tgccaaatat tacctgactg 541 acattgaccg catcgccaca ccatcattcg tgcctaccca acaagatgtg cttcgcgtcc 601 gagtgcccac caccggcatc attgagtatc catttgactt ggaaaacatc atctttcgga 661 tggtggatgt tggtggccaa cgatcggaaa gacggaagtg gattcactgc tttgagagtg 721 tcacctccat tattttcttg gttgcTCTGA GTGAATATGA CCAGGTCCTG GCTGAGTGTG 781 ACAACGAGAA TCGCATGGAA GAGAGCAAAG CCTTATTTAA AACCATCATC ACCTACCCCT 841 GGTTTCTGAA TTCGTCTGTG ATTTTATTCT TGAACAAGAA GGATCTTTTG GAAGAGAAAA 901 TCATGTACTC TCATCTAATT AGCTATTTCC CAGAATACAC AGGACCGAAA CAGGATGTCA 961 GAGCTGCCAG AGACTTTATC CTGAAGCTTT ACCAAGATCA GAATCCTGAC AAAGAGAAAG 1021 TCATCTACTC TCACTTCACA TGTGCTACAG ATACAGACAA TATTCGCTTT GTGTTTGCTG 1081 CTGTCAAAGA CACAATTCTA CAGCTAAACC TAAGGGATTC AACTTGTCTA CCCAACTTTC 1141 TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT 1201 ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 1261 TGTGGAAAGG ACGAAAGCAC GTCTTTTGCC CATGGCGCAC GCGTTAAGTC gacaatcaac 1321 ctctggatta caaaatttgt gaaagatt