Construct: ORF TRCN0000469617
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008338.1_s317c1
- Derived from:
- ccsbBroadEn_13476
- DNA Barcode:
- ACGTTGTCTGCGCGAAAAAGGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAMDC2 (256691)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469617
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256691 | MAMDC2 | MAM domain containing 2 | NM_153267.5 | 33.8% | 33.8% | 1_1362del |
| 2 | human | 256691 | MAMDC2 | MAM domain containing 2 | NR_125850.1 | 19.1% | 1_1979del;2263_2264insGGTA;2672_3614del | |
| 3 | human | 256691 | MAMDC2 | MAM domain containing 2 | XM_011518471.2 | 17.5% | 14.2% | (many diffs) |
| 4 | human | 256691 | MAMDC2 | MAM domain containing 2 | NM_001347990.1 | 15.6% | 13.8% | (many diffs) |
| 5 | human | 256691 | MAMDC2 | MAM domain containing 2 | XM_011518475.3 | 14% | 13.8% | (many diffs) |
| 6 | mouse | 71738 | Mamdc2 | MAM domain containing 2 | NM_174857.3 | 29.5% | 30.6% | (many diffs) |
| 7 | mouse | 71738 | Mamdc2 | MAM domain containing 2 | XM_006527345.3 | 14.9% | 13.3% | (many diffs) |
| 8 | mouse | 71738 | Mamdc2 | MAM domain containing 2 | XM_006527346.2 | 12% | 12.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 762
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca agctgaaatc acctttaaga agcccatgcc taccaaggtg gttttcatga 121 gcctatgcaa aagtttctgg gactgtgggc ttgtagccct ggatgacatt acaatacaat 181 tgggaagctg ctcatcttca gagaaacttc cacctccacc tggagagtgt actttcgagc 241 aagatgaatg tacatttact caggagaaaa gaaaccggag cagctggcac aggaggaggg 301 gagaaactcc cacttcctac acaggaccaa agggagatca cactactggg gtaggctact 361 acatgtacat tgaggcctcc catatggtgt atggacaaaa agcacgcctc ttgtccaggc 421 ctcTGCGAGG AGTCTCTGGA AAACACTGCT TGACCTTTTT CTACCACATG TATGGAGGGG 481 GCACTGGCCT GCTGAGTGTT TATCTGAAAA AGGAAGAAGA CAGTGAAGAG TCCCTCTTAT 541 GGAGGAGAAG AGGTGAACAG AGCATTTCCT GGCTACGAGC ACTGATTGAA TACAGCTGTG 601 AGAGGCAACA CCAGATAATT TTTGAAGCCA TTCGAGGAGT ATCAATAAGA AGTGATATTG 661 CCATTGATGA TGTTAAATTT CAGGCAGGAC CCTGTGGAGA AATGGAAGAT ACAACTCAAC 721 AATCATCAGG ATATTCTGAG GACTTAAATG AAATTGAGTA TTACCCAACT TTCTTGTACA 781 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 841 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 901 AGGACGAACG TTGTCTGCGC GAAAAAGGCG GACGCGTTAA GTCgacaatc aacctctgga 961 ttacaaaatt tgtgaaagat t