Construct: ORF TRCN0000469642
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016940.1_s317c1
- Derived from:
- ccsbBroadEn_14108
- DNA Barcode:
- ATAAGGCCGCCTTCACTAAACTGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SLC13A4 (26266)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469642
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26266 | SLC13A4 | solute carrier family 13 me... | NM_012450.4 | 99.8% | 2% | 12delG;734C>G |
| 2 | human | 26266 | SLC13A4 | solute carrier family 13 me... | NM_001318192.2 | 99.7% | 2% | 12delG;594_596delCAG;737C>G |
| 3 | human | 26266 | SLC13A4 | solute carrier family 13 me... | XM_011516024.3 | 92.3% | 2.2% | (many diffs) |
| 4 | human | 26266 | SLC13A4 | solute carrier family 13 me... | XM_017011962.1 | 84.9% | 1.7% | (many diffs) |
| 5 | human | 26266 | SLC13A4 | solute carrier family 13 me... | XM_017011963.2 | 59.3% | 3.4% | (many diffs) |
| 6 | mouse | 243755 | Slc13a4 | solute carrier family 13 (s... | NM_172892.3 | 86% | 1.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 186
- ORF length:
- 120
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cctgctcagg gcctgctccg agtccggaag ctgctgctgg tcgtctgcgt 121 cccgctcctg ctgctgcctc tgcccgtcct ccaccccagc agcgaggcct cgtgtgctta 181 cgtgctgatc gtgactgctg tgtactgggt gtcggaggca gtgcctctgg gagctgcagc 241 cctggtgccg gccttccttt acccgttctt cggagtcctc cggtccaatg aggtggcggc 301 ggagtacttc aagaacacca cgctgctgct ggtgggggtc atctgcgtgg cggctgccgt 361 ggagaagtgg aacctgcata agcgcattgc tctgcgcatg gtcttgatgg ccggagccaa 421 gccgggcatg ctgctgctct gcttcatgtg ctgtaccacg ttgctgtcca tgtggctgtc 481 caacacctcc accaccgcca tggtgatgcc catcgtggag gccgtgctgc aggagctggt 541 cagtgctgag gacgagcagc tcgtggcggg caactccaac accgaagagg ccgaacccat 601 cagtctggat gtaaagaaca gccaaccttc tctggaactc atctttgtca atgaagagtc 661 caacgcagac ctcaccactc tgatgcacaa cgagaacctg aatggtgtgc cctcgatcac 721 caaccccatc aaaactgcaa accaacacca gggcaagaag caacacccat cccaggaaaa 781 gccacaagtc ctgacccgca gccccaggaa gcagaagctg aacagaaagt acaggtccca 841 ccatgaccag atgatctgca agtgcctctc cctgagcata tcctactccg ctaccattgg 901 cggcctgacc accatcatcg gcacctccac cagcctcatc ttcctggaac acttcaacaa 961 ccagtatcca gccgcagagg tggtgaactt tggcacctgg ttcctcttca gcttccccat 1021 atccctcatc atgctggtgg tcagctggtt ctggatgcac tggctgttcc tgggctgcaa 1081 ttttaaagag acctgctctc tgagcaagaa gaagaagacc aaaagggaac agttgtcaga 1141 gaagaggatc caagaagaat atgaaaaact gggagacatt agctacccag aaatggtgac 1201 tggatttttc ttcatcctga tgaccgtact gtggtttacc cgggagcctg gctttgtccc 1261 tggctgggat tctttctttg aaaagaaagg ctaccgtact gatgccacag tctctgtctt 1321 ccttggcttc ctcctcttcc tcattccagc gaagaagccc tgctttggga aaaagaatga 1381 tggagagaac caggagcact cactggggac cgagcccatc atcacgtgga aggacttcca 1441 gaagaccatg ccctgggaga ttgtcattct ggttggggga ggctatgctc tggcttctgg 1501 tagcaagagc tctggcctct ctacatggat tgggaaccag atgttgtccc TGAGCAGCCT 1561 CCCACCGTGG GCTGTCACCC TGCTGGCATG CATCCTCGTG TCCATTGTCA CTGAGTTTGT 1621 GAGCAACCCA GCAACCATCA CCATCTTCCT GCCCATCCTG TGCAGCCTGT CTGAAACGCT 1681 GCACATTAAC CCCCTCTACA CCCTGATCCC AGTCACCATG TGCATCTCCT TTGCAGTGAT 1741 GCTGCCTGTG GGCAATCCCC CTAATGCCAT CGTCTTCAGC TATGGGCACT GCCAGATCAA 1801 AGATATGGTG AAAGCTGGCC TGGGAGTCAA CGTTATTGGA CTGGTGATAG TAATGGTGGC 1861 CATCAACACC TGGGGAGTTA GCCTCTTCCA CCTGGACACT TACCCAGCAT GGGCGAGGGT 1921 CAGCAACATC ACTGATCAAG CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 1981 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 2041 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT AAGGCCGCCT 2101 TCACTAAACT GAACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 2161 tt