Construct: ORF TRCN0000469669
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002260.1_s317c1
- Derived from:
- ccsbBroadEn_03720
- DNA Barcode:
- GACGATCAAGCCGATCGCTGGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPAN (56342)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469669
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56342 | PPAN | peter pan homolog | NM_020230.7 | 100% | 100% | |
| 2 | human | 56342 | PPAN | peter pan homolog | NM_001346139.1 | 99.7% | 99.7% | 590_591insTAG |
| 3 | human | 692312 | PPAN-P2RY11 | PPAN-P2RY11 readthrough | NM_001198690.2 | 90% | 86.9% | (many diffs) |
| 4 | human | 56342 | PPAN | peter pan homolog | NM_001346141.1 | 88.7% | 88.7% | 0_1ins159 |
| 5 | human | 692312 | PPAN-P2RY11 | PPAN-P2RY11 readthrough | NM_001040664.3 | 56.9% | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1485
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg acagtcaggg aggtcccggc accagaagcg cgcccgcgcc caggcgcagc 121 tccgcaacct cgaggcctat gccgcgaacc cgcactcgtt cgtgttcacg cgaggctgca 181 cgggtcgcaa catccggcag ctcagcctgg acgtgcggcg ggtcatggag ccgctcactg 241 ccagccgtct gcaggttcgt aagaagaact cgctgaagga ctgcgtggca gtggctgggc 301 ccctcggggt cacacacttt ctgatcctga gcaaaacaga gaccaatgtc tactttaagc 361 tgatgcgcct cccaggaggc cccaccttga ccttccaggt caagaagtac tcgctggtgc 421 gtgatgtggt ctcctcactg cgccggcacc gcatgcacga gcagcagttt gcccacccac 481 ccctcctggt actcaacagc tttggccccc atggtatgca tgtgaagctc atggccacca 541 tgttccagaa cctgttcccc tccatcaacg tgcacaaggt gaacctgaac accatcaagc 601 gctgcctcct catcgactac aaccccgact cccaggagct ggacttccgc cactatagca 661 tcaaagttgt tcctgtgggc gcgagtcgcg ggatgaagaa gctgctccag gagaagttcc 721 ccaacatgag ccgcctgcag gacatcagcg agctgctggc cacgggcgcg gggctgtcgg 781 agagcgaggc agagcctgac ggcgaccaca acatcacaga gctgcctcag gctgtcgctg 841 gccgtggcaa catgcgggcc cagcagagtg cagtgcggct caccgagatc ggcccgcgga 901 tgacactgca gctcatcaag gtccaggagg gcgtcgggga gggcaaagtg atgttccaca 961 gttttgtgag caagacggag gaggagctgc aggccatcct ggaagccaag gagaagaagc 1021 tgcggctgaa ggcgcagagg caggcccagc aggcccagaa tgtgcagcgc aagcaggagc 1081 agcgggaggc ccacagaaag aagagccTGG AGGGCATGAA GAAGGCACGG GTCGGGGGTA 1141 GTGATGAAGA GGCCTCTGGG ATCCCTTCAA GGACGGCGAG CCTGGAGTTG GGTGAGGACG 1201 ATGATGAACA GGAAGATGAT GACATCGAGT ATTTCTGCCA GGCGGTGGGC GAGGCGCCCA 1261 GTGAGGACCT GTTCCCCGAG GCCAAGCAGA AACGGCTTGC CAAGTCTCCA GGGCGGAAGC 1321 GGAAGCGGTG GGAAATGGAT CGAGGCAGGG GTCGCCTTTG TGACCAGAAG TTTCCCAAGA 1381 CCAAGGACAA GTCCCAGGGA GCCCAGGCCA GGCGGGGGCC CAGAGGGGCT TCCCGGGATG 1441 GTGGGCGAGG CCGGGGCCGG GGCCGCCCAG GGAAGAGAGT GGCCTGCCCA ACTTTCTTGT 1501 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1561 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1621 GAAAGGACGA GACGATCAAG CCGATCGCTG GTACACGCGT TAAGTCgaca atcaacctct 1681 ggattacaaa atttgtgaaa gatt