Construct: ORF TRCN0000469703
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017172.1_s317c1
- Derived from:
- ccsbBroadEn_10090
- DNA Barcode:
- CTAATGACGTGGACCACCAACCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KMT5A (387893)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469703
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_020382.7 | 99.9% | 99.7% | 947C>G |
| 2 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001367386.1 | 98.2% | 98% | 0_1insATGGCTAGAGGCAGGAAG;929C>G |
| 3 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001324504.2 | 91.3% | 91.1% | 26_27ins90;857C>G |
| 4 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001367388.1 | 88.4% | 86.2% | (many diffs) |
| 5 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001324505.2 | 83.7% | 83.5% | 0_1ins171;776C>G |
| 6 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001324506.1 | 69.2% | 69% | 0_1ins324;623C>G |
| 7 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001367389.1 | 69.2% | 69% | 0_1ins324;623C>G |
| 8 | human | 387893 | KMT5A | lysine methyltransferase 5A | NR_136910.2 | 36.5% | (many diffs) | |
| 9 | mouse | 67956 | Kmt5a | lysine methyltransferase 5A | NM_030241.3 | 89% | 91.4% | (many diffs) |
| 10 | mouse | 67956 | Kmt5a | lysine methyltransferase 5A | NM_001310723.1 | 81.4% | 77.5% | (many diffs) |
| 11 | mouse | 67956 | Kmt5a | lysine methyltransferase 5A | NM_001310725.1 | 74.1% | 77.2% | (many diffs) |
| 12 | mouse | 67956 | Kmt5a | lysine methyltransferase 5A | NM_001310727.1 | 74.1% | 77.2% | (many diffs) |
| 13 | mouse | 67956 | Kmt5a | lysine methyltransferase 5A | XM_006530450.3 | 74.1% | 77.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tagaggcagg aagatgtcca agccccgcgc ggtggaggcg gcggcggcgg 121 cggcggcggt ggcagcgacg gccccgggcc cggagatggt ggagcggagg ggcccgggga 181 ggccccgcac cgacggggag aacgtattta ccgggcagtc aaagatctat tcctacatga 241 gcccgaacaa atgctctgga atgcgtttcc cccttcagga agagaactca gttacacatc 301 acgaagtcaa atgccagggg aaaccattag ccggaatcta caggaaacga gaagagaaaa 361 gaaatgctgg gaacgcagta cggagcgcca tgaagtccga ggaacagaag atcaaagacg 421 ccaggaaagg tcccctggta ccttttccaa accaaaaatc tgaagcagca gaacctccaa 481 aaactccacc ctcatcttgt gattccacca atgcagccat cgccaagcaa gccctgaaaa 541 agcccatcaa gggcaaacag gccccccgaa aaaaagctca aggaaaaacg caacagaatc 601 gcaaacttac ggatttctac cctgtccgaa ggagcTCCAG GAAGAGCAAA GCCGAGCTGC 661 AGTCTGAAGA AAGGAAAAGA ATAGATGAAT TGATTGAAAG TGGGAAGGAA GAAGGAATGA 721 AGATTGACCT CATCGATGGC AAAGGCAGGG GTGTGATTGC CACCAAGCAG TTCTCCCGGG 781 GTGACTTTGT GGTGGAATAC CACGGGGACC TCATCGAGAT CACCGACGCC AAGAAACGGG 841 AGGCTCTGTA CGCACAGGAC CCTTCCACGG GCTGCTACAT GTACTATTTT CAGTATCTGA 901 GCAAAACCTA CTGCGTGGAT GCAACTAGAG AGACAAATCG CCTAGGAAGA CTGATCAATC 961 ACAGCAAATG TGGGAACTGC CAAACCAAAC TGCACGACAT CGACGGCGTA CGTCACCTCA 1021 TCCTCATCGC CTCCCGAGAC ATCGCGGCTG GGGAGGAGCT CCTGTATGAC TATGGGGACC 1081 GCAGCAAGGC TTCCATTGAA GCCCACCCGT GGCTGAAGCA TTACCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGACTA ATGACGTGGA CCACCAACCC GACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t