Construct: ORF TRCN0000469713
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007543.1_s317c1
- Derived from:
- ccsbBroadEn_12641
- DNA Barcode:
- CAATCGAATCGGACAGACAATCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPCAT1 (79888)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469713
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79888 | LPCAT1 | lysophosphatidylcholine acy... | XM_005248373.3 | 80% | 80% | 1_291del |
| 2 | human | 79888 | LPCAT1 | lysophosphatidylcholine acy... | XM_011514134.1 | 78.9% | 78.9% | 1_312del |
| 3 | human | 79888 | LPCAT1 | lysophosphatidylcholine acy... | XM_017009879.2 | 74.5% | 72.3% | (many diffs) |
| 4 | human | 79888 | LPCAT1 | lysophosphatidylcholine acy... | NM_024830.5 | 72.8% | 72.8% | 1_435del |
| 5 | human | 79888 | LPCAT1 | lysophosphatidylcholine acy... | XM_011514132.1 | 68.1% | 68.1% | 1_546del |
| 6 | mouse | 210992 | Lpcat1 | lysophosphatidylcholine acy... | XM_006517188.2 | 74.2% | 79.1% | (many diffs) |
| 7 | mouse | 210992 | Lpcat1 | lysophosphatidylcholine acy... | NM_145376.5 | 60.1% | 64% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1233
- ORF length:
- 1167
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gatgtcctcc atcgtgatga aggcagagag cagagacatc ccgatctggg 121 gaactctgat ccagtatata cggcctgtgt tcgtgtcccg gtcagaccag gattctcgca 181 ggaaaacagt agaagaaatc aagagacggg cgcagtccaa cggaaagtgg ccacagataa 241 tgatttttcc agaaggaact tgtacaaaca ggacctgcct aattaccttc aaacctggtg 301 cattcatccc tggagcgccc gtccagcctg tggttttacg atatccaaat aaactggaca 361 ccatcacatg gacgtggcaa ggacctggag cgctggaaat cctgtggctc acgctgtgtc 421 agtttcacaa ccaagtggaa atcgagttcc ttcctgtgta cagcccttct gaggaggaga 481 agaggaaccc cgcgctgtat gccagcaacg tgcggcgagt catggccgag gccttgggtg 541 tctccgtgac tgactacacg ttcgaggact gccagctggc cctggcggaa ggacagctcc 601 gtctccccgc tgacacttgc cttttagaat ttgccaggct cgtgcggggc ctcgggctaa 661 aaccagaaaa gcttgaaaaa gatctggaca gatactcaga aagagccagg atgaagggag 721 gagagaagat aggtattgcg gagtttgccg cctccctgga agtccccgtt tctgacttgc 781 tggaagacat gttttcactg ttcgacgaga gcggcagcgg cgaggtggac ctgcgagagt 841 gtgtggttgc cctgtctgtc gtctgccggc cggcccggac cctggacacc atccagctgg 901 ctttcaagat gtacggagcg caagaggacg gcagcgtcgg cgaaggtgac ctgtcctgca 961 tcctcaagac ggccctgggg gtggcagagc tcaccgtgac cgacctattc cgagccattg 1021 accaagagga gaaggggaag atcacattcg ctgacttcca caggtttgca gaaatgtacc 1081 ctgccttcgc agaggaatac ctgtacccgg atcagacaca tttcgaaagc tgtgcagaga 1141 cctcacctgc gccaatccca aacggcttct gtgccgattt cagcccggaa aacTCAGACG 1201 CTGGGCGGAA GCCTGTTCGC AAGAAGCTGG ATTACCCAAC TTTCTTGTAC AAAGTGGTTG 1261 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1321 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 1381 ATCGAATCGG ACAGACAATC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1441 ttgtgaaaga tt