Transcript: Human XM_005248373.3

PREDICTED: Homo sapiens lysophosphatidylcholine acyltransferase 1 (LPCAT1), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
LPCAT1 (79888)
Length:
3854
CDS:
170..1630

Additional Resources:

NCBI RefSeq record:
XM_005248373.3
NBCI Gene record:
LPCAT1 (79888)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005248373.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231881 GACTTAGAGTTGGCGGATATT pLKO_005 3617 3UTR 100% 13.200 18.480 N LPCAT1 n/a
2 TRCN0000231879 AGATAGGTATTGCGGAGTTTG pLKO_005 1122 CDS 100% 10.800 15.120 N LPCAT1 n/a
3 TRCN0000056068 CGGATCAGACACATTTCGAAA pLKO.1 1503 CDS 100% 4.950 3.960 N LPCAT1 n/a
4 TRCN0000231880 TACCCGGATCAGACACATTTC pLKO_005 1499 CDS 100% 0.000 0.000 N LPCAT1 n/a
5 TRCN0000231877 GGAACTCTGATCCAGTATATA pLKO_005 515 CDS 100% 15.000 10.500 N LPCAT1 n/a
6 TRCN0000231878 ACGGAAAGTGGCCACAGATAA pLKO_005 615 CDS 100% 13.200 9.240 N LPCAT1 n/a
7 TRCN0000056070 CCAGTATATACGGCCTGTGTT pLKO.1 526 CDS 100% 4.950 3.465 N LPCAT1 n/a
8 TRCN0000056069 GAAGATCACATTCGCTGACTT pLKO.1 1432 CDS 100% 4.950 3.465 N LPCAT1 n/a
9 TRCN0000056071 GAAGATAGGTATTGCGGAGTT pLKO.1 1120 CDS 100% 4.050 2.835 N LPCAT1 n/a
10 TRCN0000056072 CGCGCCTCACTCGTCCTACTT pLKO.1 421 CDS 100% 0.000 0.000 N LPCAT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005248373.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12641 pDONR223 100% 80% 80% None 1_291del n/a
2 ccsbBroad304_12641 pLX_304 0% 80% 80% V5 1_291del n/a
3 TRCN0000469713 CAATCGAATCGGACAGACAATCCG pLX_317 4.2% 80% 80% V5 1_291del n/a
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