Construct: ORF TRCN0000469723

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012079.1_s317c1
Derived from:
ccsbBroadEn_03529
DNA Barcode:
TTCATGCCAGTACTAGGCCGATGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIWIL2 (55124)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469723
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55124 PIWIL2 piwi like RNA-mediated gene... NM_001135721.2 100% 100%
2 human 55124 PIWIL2 piwi like RNA-mediated gene... NM_018068.5 100% 100%
3 human 55124 PIWIL2 piwi like RNA-mediated gene... NM_001330480.2 96.3% 96.3% 2656_2657ins108
4 human 55124 PIWIL2 piwi like RNA-mediated gene... XM_005273551.4 82.5% 82.3% (many diffs)
5 mouse 57746 Piwil2 piwi-like RNA-mediated gene... NM_021308.1 85.5% 87.9% (many diffs)
6 mouse 57746 Piwil2 piwi-like RNA-mediated gene... XM_006519329.3 85.5% 87.9% (many diffs)
7 mouse 57746 Piwil2 piwi-like RNA-mediated gene... XM_006519328.1 79.7% 81.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2985
ORF length:
2919
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tcctttccga ccatcgttca ggggccagtc tcctatccac ccatcccagt
121gccaggctgt acggatgcca ggctgttggc cacaagcttc taaacctttg gacccagctc
181tgggcagggg agcacctgca ggcagaggcc atgtatttgg aaagccagag gaaccaagca
241cacagagggg gccagcacaa agggagtctg tgggtttggt ctccatgttc cgaggcctgg
301gcattgaaac agtttctaag acccctctga aacgggaaat gcttccatca ggtagaggca
361ttttaggtcg aggcttgtct gctaatctgg tacgcaagga cagggaggaa ctctctccca
421ctttttggga tccaaaagtg ttggcggctg gggacagcaa gatggcagag acctccgttg
481gttggagtag gacgcttgga agagggagtt cagatgcgtc tttattacca ctgggaagag
541cagcaggtgg tatcagcaga gaagtggaca agcctccctg taccttcagc acaccgtccc
601ggggtccccc gcagctgtca tcaccaccag ctctgcccca gtctcccctg cactctccag
661atcgccctct ggtcctgact gtggaacaca aggaaaaaga gcttattgtg aagcaaggat
721caaaaggaac acctcagtct ttgggactga acctcgtcaa aatacagtgt cataatgaag
781cagtttatca atatcatgtg actttcagcc ccaatgtgga gtgcaaaagc atgaggttcg
841gcatgttgaa ggaccatcaa gctgtcaccg gcaacgtcac tgcgtttgat ggatctattc
901tctatctgcc tgttaagctt caacaagttc ttgagttaaa aagtcaaagg aaaacagaca
961gtgctgaaat cagcattaag attcagatga caaagatcct ggagccctgc tctgacctgt
1021gcattccctt ctacaatgtt gttttccgtc gggtaatgaa acttttagat atgaagcttg
1081tggggagaaa cttttatgac cctacaagtg ctatggtact acagcaacac agattgcaga
1141tctggccagg ctatgcagct agcatccgaa ggacagatgg agggctcttc ctgctagctg
1201atgtctccca taaggtcatt cggaatgact gtgtgctgga tgtcatgcat gccatttatc
1261agcagaataa agaacacttc caggatgagt gtactaagct tctggttggc aatattgtta
1321tcacccgata taacaatcgt acctatcgta ttgatgatgt ggattggaat aagactccaa
1381aggatagctt cacgatgtct gatgggaaag agatcacatt cttggaatac tacagcaaaa
1441attatgggat cacagttaag gaagaggacc agccattgct gattcacagg cccagtgaga
1501gacaggataa tcatgggatg ctgctaaaag gggaaatcct gctgctgcct gagctttctt
1561ttatgaccgg aatcccagag aagatgaaga aggacttcag agccatgaag gatttggctc
1621agcaaatcaa tctgagcccc aagcaacacc atagtgcttt ggaatgcttg ctgcaaagaa
1681ttgcaaagaa cgaggcagcc accaatgaac tgatgcgttg ggggctccgt ctgcaaaagg
1741atgtacataa gattgaagga cgtgttctgc caatggaaag aattaactta aaaaatactt
1801cgtttatcac atctcaggaa ctaaactggg ttaaggaagt aaccagagac ccttccatct
1861tgactatccc catgcatttc tgggcacttt tttacccaaa gagagcaatg gaccaggctc
1921gagaactggt caacatgttg gagaagatag ccggccccat tggcatgcgt atgagcccac
1981cggcctgggt tgaactaaag gatgaccgaa tagagactta tgtcagaacc attcaatcca
2041cgttaggagc tgaggggaag atacagatgg ttgtttgcat catcatgggc ccacgtgatg
2101atctctatgg ggccatcaag aagctgtgct gtgtgcagtc cccagtgccc tcccaggttg
2161tcaatgttcg aaccattggt cagcccacca ggcttcggag tgtggcccag aagattttac
2221ttcagattaa ctgtaaattg ggtggtgagc tctggggagt ggatattcct ctgaaacagt
2281taatggtgat cgggatggat gtttaccatg accccagtag aggcatgcgc tccgtggttg
2341gcttcgtggc aagcatcaat ctcaccctca caaaatggta ttcccgggtg gtgttccaga
2401tgccgcatca ggagattgtg gacagcctga agctatgcct cgtgggctcc ttaaaaaagt
2461tttatgaggt gaaccactgt ctaccagaga agattgtggt gtaccgtgat ggagtgtctg
2521atggccaact gaagacagtt gccaactatg agattcctca actacagaag tgttttgaag
2581cttttgagaa ttatcagccc aagatggtgg tgtttgtagt tcagaagaaa atcagtacta
2641atctatatct ggctgctcct cagaactttg taactcccac tcctggaact gtggtagatc
2701atacaataac aagctgtgag tgggtggatt tctatcttct tgcccatcat gtacggcagg
2761gctgtggcat tcctacgcat tatgtctgtg ttctcaacac cgcaaacctg agccctgatc
2821atatgcagag gctgactttc aaactgtgcc acatgtactg gaattggcct ggcaccatca
2881gagttccagc tccttgcaag tatgcccaca agctagcttt cctgtcagga cacatcttgc
2941atcatgaacc agccatccag ctgtgcgaga acctgttcTT CCTGTGCCCA ACTTTCTTGT
3001ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
3061AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
3121GAAAGGACGA TTCATGCCAG TACTAGGCCG ATGTACGCGT TAAGTCgaca atcaacctct
3181ggattacaaa atttgtgaaa gatt

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