Construct: ORF TRCN0000469728
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011811.3_s317c1
- Derived from:
- ccsbBroadEn_14729
- DNA Barcode:
- ATTCGTGTTTCAAGTAAGTGGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCK2 (5106)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469728
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5106 | PCK2 | phosphoenolpyruvate carboxy... | NM_004563.4 | 100% | 100% | |
| 2 | human | 5106 | PCK2 | phosphoenolpyruvate carboxy... | NM_001291556.2 | 79% | 79% | 0_1ins402 |
| 3 | human | 5106 | PCK2 | phosphoenolpyruvate carboxy... | NM_001308054.2 | 79% | 79% | 0_1ins402 |
| 4 | human | 5106 | PCK2 | phosphoenolpyruvate carboxy... | XM_006720158.3 | 77.4% | 76.5% | (many diffs) |
| 5 | human | 5106 | PCK2 | phosphoenolpyruvate carboxy... | NM_001018073.3 | 67.6% | 64.9% | (many diffs) |
| 6 | mouse | 74551 | Pck2 | phosphoenolpyruvate carboxy... | NM_028994.2 | 85.1% | 88.4% | (many diffs) |
| 7 | mouse | 74551 | Pck2 | phosphoenolpyruvate carboxy... | XM_006519632.2 | 71.8% | 73.1% | (many diffs) |
| 8 | mouse | 74551 | Pck2 | phosphoenolpyruvate carboxy... | XM_006519633.2 | 49.5% | 52.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1986
- ORF length:
- 1920
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcattgtac cgccctggcc tgcggcttaa ctggcatggg ctgagcccct 121 tgggctggcc atcatgccgt agcatccaga ccctgcgagt gcttagtgga gatctgggcc 181 agcttcccac tggcattcga gattttgtag agcacagtgc ccgcctgtgc caaccagagg 241 gcatccacat ctgtgatgga actgaggctg agaatactgc cacactgacc ctgctggagc 301 agcagggcct catccgaaag ctccccaagt acaataactg ctggctggcc cgcacagacc 361 ccaaggatgt ggcacgagta gagagcaaga cggtgattgt aactccttct cagcgggaca 421 cggtaccact cccgcctggt ggggcccgtg ggcagctggg caactggatg tccccagctg 481 atttccagcg agctgtggat gagaggtttc caggctgcat gcagggccgc accatgtatg 541 tgcttccatt cagcatgggt cctgtgggct ccccgctgtc ccgcatcggg gtgcagctca 601 ctgactcagc ctatgtggtg gcaagcatgc gtattatgac ccgactgggg acacctgtgc 661 ttcaggccct gggagatggt gactttgtca agtgtctgca ctccgtgggc cagcccctga 721 caggacaagg ggagccagtg agccagtggc cgtgcaaccc agagaaaacc ctgattggcc 781 acgtgcccga ccagcgggag atcatctcct tcggcagcgg ctatggtggc aactccctgc 841 tgggcaagaa gtgctttgcc ctacgcatcg cctctcggct ggcccgggat gagggctggc 901 tggcagagca catgctgatc ctgggcatca ccagccctgc agggaagaag cgctatgtgg 961 cagccgcctt ccctagtgcc tgtggcaaga ccaacctggc tatgatgcgg cctgcactgc 1021 caggctggaa agtggagtgt gtgggggatg atattgcttg gatgaggttt gacagtgaag 1081 gtcgactccg ggccatcaac cctgagaacg gcttctttgg ggttgcccct ggtacctctg 1141 ccaccaccaa tcccaacgcc atggctacaa tccagagtaa cactattttt accaatgtgg 1201 ctgagaccag tgatggtggc gtgtactggg agggcattga ccagcctctt ccacctggtg 1261 ttactgtgac ctcctggctg ggcaaaccct ggaaacctgg tgacaaggag ccctgtgcac 1321 atcccaactc tcgattttgt gccccggctc gccagtgccc catcatggac ccagcctggg 1381 aggccccaga gggtgtcccc attgacgcca tcatctttgg tggccgcaga cccaaagggg 1441 tacccctggt atacgaggcc ttcaactggc gtcatggggt gtttgtgggc agcgccatgc 1501 gctctgagtc cactgctgca gcagaacaca aagggaagat catcatgcac gacccatttg 1561 ccatgcggcc cttttttggc tacaacttcg ggcactacct ggaacactgg ctgagcatgg 1621 aagggcgcaa gggggcccag ctgccccgta TCTTCCATGT CAACTGGTTC CGGCGTGACG 1681 AGGCAGGGCA CTTCCTGTGG CCAGGCTTTG GGGAGAATGC TCGGGTGCTA GACTGGATCT 1741 GCCGGCGGTT AGAGGGGGAG GACAGTGCCC GAGAGACACC CATTGGGCTG GTGCCAAAGG 1801 AAGGAGCCTT GGATCTCAGC GGCCTCAGAG CTATAGACAC CACTCAGCTG TTCTCCCTCC 1861 CCAAGGACTT CTGGGAACAG GAGGTTCGTG ACATTCGGAG CTACCTGACA GAGCAGGTCA 1921 ACCAGGATCT GCCCAAAGAG GTGTTGGCTG AGCTTGAGGC CCTGGAGAGA CGTGTGCACA 1981 AAATGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2041 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2101 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATTCGTGTT TCAAGTAAGT GGTAGACGCG 2161 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt