Transcript: Mouse NM_028994.2

Mus musculus phosphoenolpyruvate carboxykinase 2 (mitochondrial) (Pck2), mRNA.

Source:
NCBI, updated 2017-06-25
Taxon:
Mus musculus (mouse)
Gene:
Pck2 (74551)
Length:
3400
CDS:
139..2142

Additional Resources:

NCBI RefSeq record:
NM_028994.2
NBCI Gene record:
Pck2 (74551)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_028994.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000088647 GCACATCCAAACTCGCGCTTT pLKO.1 1471 CDS 100% 4.050 5.670 N Pck2 n/a
2 TRCN0000334130 GCACATCCAAACTCGCGCTTT pLKO_005 1471 CDS 100% 4.050 5.670 N Pck2 n/a
3 TRCN0000088646 AGACGGTGATTGTAACTCCTT pLKO.1 542 CDS 100% 2.640 3.696 N Pck2 n/a
4 TRCN0000334129 AGACGGTGATTGTAACTCCTT pLKO_005 542 CDS 100% 2.640 3.696 N Pck2 n/a
5 TRCN0000374465 GGTGGCCAGTATGCGTATTAT pLKO_005 771 CDS 100% 15.000 12.000 N Pck2 n/a
6 TRCN0000364023 TCTGTGTTTGAGTTAGTATTT pLKO_005 2580 3UTR 100% 13.200 9.240 N Pck2 n/a
7 TRCN0000088645 CCTATTGATGCCATCATCTTT pLKO.1 1552 CDS 100% 5.625 3.938 N Pck2 n/a
8 TRCN0000088643 GCTCTGTGTTTGAGTTAGTAT pLKO.1 2578 3UTR 100% 5.625 3.938 N Pck2 n/a
9 TRCN0000374466 GACTGGATCTGCCGAAGATTA pLKO_005 1885 CDS 100% 13.200 7.920 N Pck2 n/a
10 TRCN0000088644 CCTCGTATCTTCCATGTCAAT pLKO.1 1798 CDS 100% 4.950 2.970 N Pck2 n/a
11 TRCN0000363752 CCTCGTATCTTCCATGTCAAT pLKO_005 1798 CDS 100% 4.950 2.970 N Pck2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_028994.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06695 pDONR223 100% 85.1% 88.4% None (many diffs) n/a
2 ccsbBroad304_06695 pLX_304 0% 85.1% 88.4% V5 (many diffs) n/a
3 TRCN0000474518 ATCTCACTTGAACCCTGTGTCAAT pLX_317 23.1% 85.1% 88.4% V5 (many diffs) n/a
4 ccsbBroadEn_14729 pDONR223 0% 85.1% 88.4% None (many diffs) n/a
5 ccsbBroad304_14729 pLX_304 0% 85.1% 88.4% V5 (many diffs) n/a
6 TRCN0000469728 ATTCGTGTTTCAAGTAAGTGGTAG pLX_317 17.8% 85.1% 88.4% V5 (many diffs) n/a
7 TRCN0000488002 GCACTAATTCCGCCGAGACGGGCG pLX_317 8.9% 85.1% 88.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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