Transcript: Human NM_001291556.2

Homo sapiens phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
PCK2 (5106)
Length:
2013
CDS:
332..1852

Additional Resources:

NCBI RefSeq record:
NM_001291556.2
NBCI Gene record:
PCK2 (5106)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145524 GCTGAATGGAAGCACATACA pXPR_003 TGG 70 5% 4 1.0305 PCK2 PCK2 78081
2 BRDN0001145460 TGCGTATTATGACCCGACTG pXPR_003 GGG 177 12% 4 0.2425 PCK2 PCK2 78080
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001291556.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195705 CCATTGACGCCATCATCTTTG pLKO.1 1263 CDS 100% 10.800 15.120 N PCK2 n/a
2 TRCN0000199662 GTTCGTGACATTCGGAGCTAC pLKO.1 1748 CDS 100% 4.050 5.670 N PCK2 n/a
3 TRCN0000312598 GGTGGCAAGCATGCGTATTAT pLKO_005 481 CDS 100% 15.000 12.000 N PCK2 n/a
4 TRCN0000052667 GAACAGGAGGTTCGTGACATT pLKO.1 1739 CDS 100% 4.950 3.960 N PCK2 n/a
5 TRCN0000380418 ACAATCCAGAGTAACACTATT pLKO_005 1031 CDS 100% 13.200 9.240 N PCK2 n/a
6 TRCN0000370248 CTGCTGCAGCAGAACACAAAG pLKO_005 1377 CDS 100% 10.800 7.560 N PCK2 n/a
7 TRCN0000052664 GCACATCCCAACTCTCGATTT pLKO.1 1181 CDS 100% 10.800 7.560 N PCK2 n/a
8 TRCN0000327658 GCACATCCCAACTCTCGATTT pLKO_005 1181 CDS 100% 10.800 7.560 N PCK2 n/a
9 TRCN0000052665 CATGGCTACAATCCAGAGTAA pLKO.1 1024 CDS 100% 4.950 3.465 N PCK2 n/a
10 TRCN0000052666 GTAGAGAGCAAGACGGTGATT pLKO.1 242 5UTR 100% 4.950 3.465 N PCK2 n/a
11 TRCN0000327657 GTAGAGAGCAAGACGGTGATT pLKO_005 242 5UTR 100% 4.950 3.465 N PCK2 n/a
12 TRCN0000052663 GCACCATGTATGTGCTTCCAT pLKO.1 393 CDS 100% 3.000 2.100 N PCK2 n/a
13 TRCN0000088646 AGACGGTGATTGTAACTCCTT pLKO.1 252 5UTR 100% 2.640 1.848 N Pck2 n/a
14 TRCN0000334129 AGACGGTGATTGTAACTCCTT pLKO_005 252 5UTR 100% 2.640 1.848 N Pck2 n/a
15 TRCN0000312597 CTAGTCTAGCAAGAGGACATA pLKO_005 1862 3UTR 100% 0.000 0.000 N PCK2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001291556.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06695 pDONR223 100% 79% 79% None 0_1ins402 n/a
2 ccsbBroad304_06695 pLX_304 0% 79% 79% V5 0_1ins402 n/a
3 TRCN0000474518 ATCTCACTTGAACCCTGTGTCAAT pLX_317 23.1% 79% 79% V5 0_1ins402 n/a
4 ccsbBroadEn_14729 pDONR223 0% 79% 79% None 0_1ins402 n/a
5 ccsbBroad304_14729 pLX_304 0% 79% 79% V5 0_1ins402 n/a
6 TRCN0000469728 ATTCGTGTTTCAAGTAAGTGGTAG pLX_317 17.8% 79% 79% V5 0_1ins402 n/a
7 TRCN0000488002 GCACTAATTCCGCCGAGACGGGCG pLX_317 8.9% 79% 79% V5 (not translated due to prior stop codon) 0_1ins402 n/a
Download CSV