Construct: ORF TRCN0000469738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015898.1_s317c1
- Derived from:
- ccsbBroadEn_12747
- DNA Barcode:
- AACCATTTTGTACGTGACACCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GUCD1 (83606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469738
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83606 | GUCD1 | guanylyl cyclase domain con... | NM_001284254.2 | 100% | 100% | |
| 2 | human | 83606 | GUCD1 | guanylyl cyclase domain con... | NM_031444.4 | 99.5% | 99.1% | 629_631delCAG |
| 3 | human | 83606 | GUCD1 | guanylyl cyclase domain con... | NM_001284255.2 | 82% | 82% | 385_386ins129 |
| 4 | human | 83606 | GUCD1 | guanylyl cyclase domain con... | NM_001284252.2 | 80.1% | 78.3% | (many diffs) |
| 5 | human | 83606 | GUCD1 | guanylyl cyclase domain con... | NM_001284251.1 | 79.8% | 77.7% | (many diffs) |
| 6 | human | 83606 | GUCD1 | guanylyl cyclase domain con... | NM_001284253.2 | 65.6% | 63.7% | (many diffs) |
| 7 | human | 83606 | GUCD1 | guanylyl cyclase domain con... | NM_001284256.2 | 56.4% | 55.2% | (many diffs) |
| 8 | human | 83606 | GUCD1 | guanylyl cyclase domain con... | NM_001284257.2 | 56.2% | 54.8% | (many diffs) |
| 9 | human | 83606 | GUCD1 | guanylyl cyclase domain con... | XM_005261761.2 | 44.9% | 42% | (many diffs) |
| 10 | mouse | 68778 | Gucd1 | guanylyl cyclase domain con... | NM_175133.2 | 92% | 95.8% | (many diffs) |
| 11 | mouse | 68778 | Gucd1 | guanylyl cyclase domain con... | NM_001347201.1 | 76.7% | 80.3% | (many diffs) |
| 12 | mouse | 68778 | Gucd1 | guanylyl cyclase domain con... | XM_006514034.3 | 76.7% | 80.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gacggaggcg gaggcagcgg ggccgccgct cgagcccggg gactttgtgc 121 aactgcctgt gcccgtcatc cagcagctct accactggga ctgtggcctg gcctgctcca 181 ggatggtgct gcggtacctg ggccagctgg acgacagtga gtttgagaga gccctgcaga 241 agctgcagct gaccaggagc atctggacca tcgacctggc ctacctgatg caccactttg 301 gcgtgaggca ccgcttctgt acccagaccc tgggtgtcga caagggctac aagaaccagt 361 ccttctacag gaagcacttt gacacagaag agacccgggt gaatcagctg tttgcacaag 421 caaaggccTG CAAGGTGCTG GTGGAGAAAT GCACAGTGAG TGTGAAGGAC ATCCAGGCGC 481 ACCTGGCTCA GGGCCATGTG GCCATCGTGC TGGTGAACTC GGGGGTGCTG CACTGTGACC 541 TGTGCTCCAG CCCTGTCAAG TACTGCTGCT TCACCCCCAG TGGCCACCAC TGCTTCTGCC 601 GCACTCCTGA CTACCAGGGC CACTTCATCG TGCTGCGTGG CTACAACCGG GCCACTGGCT 661 GCATCTTCTA CAACAACCCA GCCTATGCCG ACCGAATGTG CAGCACCAGC ATCAGTAACT 721 TTGAGGAGGC CAGAACCAGC TATGGCACAG ATGAGGACAT CCTCTTTGTC TACTTGGACA 781 GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGAAA CCATTTTGTA CGTGACACCT AAACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt