Transcript: Human NM_031444.4

Homo sapiens guanylyl cyclase domain containing 1 (GUCD1), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
GUCD1 (83606)
Length:
3438
CDS:
148..870

Additional Resources:

NCBI RefSeq record:
NM_031444.4
NBCI Gene record:
GUCD1 (83606)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_031444.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000128715 CCACAAGTTAAAGGTCACCTT pLKO.1 3075 3UTR 100% 2.640 3.696 N GUCD1 n/a
2 TRCN0000130275 CCAAGTTTCTGTGGCTCATTA pLKO.1 1407 3UTR 100% 13.200 9.240 N GUCD1 n/a
3 TRCN0000130668 CCATTGCTTTGGAGCACAGAT pLKO.1 2602 3UTR 100% 4.950 3.465 N GUCD1 n/a
4 TRCN0000130491 GAAATGCACAGTGAGTGTGAA pLKO.1 528 CDS 100% 4.950 3.465 N GUCD1 n/a
5 TRCN0000130769 GTAACTTTGAGGAGGCCAGAA pLKO.1 800 CDS 100% 4.050 2.835 N GUCD1 n/a
6 TRCN0000128026 GAACCAGTCCTTCTACAGGAA pLKO.1 435 CDS 100% 2.640 1.848 N GUCD1 n/a
7 TRCN0000128025 GACGACAGTGAGTTTGAGAGA pLKO.1 292 CDS 100% 2.640 1.848 N GUCD1 n/a
8 TRCN0000268959 TGCAGCACCAGCATCAGTAAC pLKO_005 784 CDS 100% 10.800 6.480 N Gucd1 n/a
9 TRCN0000127945 CTATGGCACAGATGAGGACAT pLKO.1 825 CDS 100% 4.050 2.430 N GUCD1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_031444.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12747 pDONR223 100% 99.5% 99.1% None 629_631delCAG n/a
2 ccsbBroad304_12747 pLX_304 0% 99.5% 99.1% V5 629_631delCAG n/a
3 TRCN0000469738 AACCATTTTGTACGTGACACCTAA pLX_317 49.5% 99.5% 99.1% V5 629_631delCAG n/a
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