Construct: ORF TRCN0000469743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002336.1_s317c1
- Derived from:
- ccsbBroadEn_01845
- DNA Barcode:
- CTCAGGTGTCATGTCGTTATGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NELFE (7936)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469743
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7936 | NELFE | negative elongation factor ... | NM_002904.6 | 100% | 100% | |
| 2 | human | 7936 | NELFE | negative elongation factor ... | XM_006715205.3 | 98.6% | 98.6% | 402_403insAGCTTTGTGTCTTCT |
| 3 | human | 7936 | NELFE | negative elongation factor ... | XM_011514913.3 | 85.5% | 85.5% | 0_1ins165 |
| 4 | human | 7936 | NELFE | negative elongation factor ... | XM_017011299.2 | 84.2% | 84.2% | 0_1ins165;237_238insAGCTTTGTGTCTTCT |
| 5 | mouse | 27632 | Nelfe | negative elongation factor ... | NM_001045863.1 | 85% | 90.5% | (many diffs) |
| 6 | mouse | 27632 | Nelfe | negative elongation factor ... | NM_001045864.1 | 85% | 90.5% | (many diffs) |
| 7 | mouse | 27632 | Nelfe | negative elongation factor ... | NM_138580.2 | 85% | 90.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1206
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ggtgataccc cccggactga gcgaggaaga ggaggctctg cagaagaaat 121 tcaacaagct caagaaaaag aaaaaggcat tgctggctct gaagaagcaa agtagcagca 181 gcacaaccag ccaaggtggt gtcaaacgct cactatcaga gcagcctgtc atggacacag 241 ccacagcaac agagcaggca aagcagctgg tgaagtcagg agccatcagt gccatcaagg 301 ctgagaccaa gaactcaggc ttcaagcgtt ctcgaaccct tgaggggaag ttaaaggacc 361 ccgagaaggg accagtcccc actttccagc cgttccagag gagcatatct gctgatgatg 421 acctgcaaga gtcatccaga cgtccccaga ggaaatctct gtatgagagc tttgtgtctt 481 ctagtgatcg acttcgagaa ctaggaccag atggagaaga ggcagagggc ccaggggctg 541 gtgatggtcc ccctcgaagc tttgactggg gctatgaaga acgcagtggt gcccactcct 601 cagcctcccc tccccgaagc cgcagccggg accgcagcca tgagaggaac cgggacagag 661 accgagatcg ggagcgggat cgagaccggg atcgagacag agacagagag cgggacaggg 721 atcgggatcg ggatcgagat cgagaccggg aacgggacag ggatcgggag cgggatcgag 781 accgagaccg agagggtcct ttccgcaggt cggattcatt cccTGAACGG CGAGCCCCTA 841 GGAAAGGGAA TACTCTCTAT GTATATGGAG AAGACATGAC ACCCACCCTT CTCCGTGGGG 901 CCTTCTCTCC TTTTGGAAAC ATCATTGACC TCTCCATGGA CCCACCCAGA AACTGTGCCT 961 TCGTCACCTA TGAAAAGATG GAGTCAGCAG ATCAGGCCGT TGCTGAGCTC AACGGGACCC 1021 AGGTGGAGTC TGTACAGCTC AAAGTCAACA TAGCCCGAAA ACAGCCCATG CTGGATGCCG 1081 CTACTGGCAA GTCTGTCTGG GGCTCCCTCG CTGTCCAGAA CAGCCCTAAG GGTTGCCACC 1141 GGGACAAGAG GACCCAGATT GTCTACAGTG ATGACGTCTA CAAGGAAAAC CTTGTGGATG 1201 GCTTCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTCAGGTGT CATGTCGTTA TGGGAACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt