Construct: ORF TRCN0000469744
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013099.1_s317c1
- Derived from:
- ccsbBroadEn_10949
- DNA Barcode:
- TAAGCACAGCGCGGATGTGGCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMX1A (4009)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469744
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4009 | LMX1A | LIM homeobox transcription ... | XM_011509538.3 | 33% | 33.1% | 1_606del;810T>C |
| 2 | human | 4009 | LMX1A | LIM homeobox transcription ... | NM_001174069.1 | 26% | 26.1% | 1_846del;1050T>C |
| 3 | human | 4009 | LMX1A | LIM homeobox transcription ... | NM_177398.4 | 26% | 26.1% | 1_846del;1050T>C |
| 4 | mouse | 110648 | Lmx1a | LIM homeobox transcription ... | NM_033652.5 | 23.6% | 25.6% | (many diffs) |
| 5 | mouse | 110648 | Lmx1a | LIM homeobox transcription ... | XM_006496611.2 | 23.6% | 25.6% | (many diffs) |
| 6 | mouse | 110648 | Lmx1a | LIM homeobox transcription ... | XM_017321684.1 | 23.6% | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 390
- ORF length:
- 300
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGAAGGAAT CATGAACCCC TACACGGCTC 121 TGCCCACCCC ACAGCAGCTC CTGGCCATCG AGCAGAGTGT CTACAGCTCA GATCCCTTCC 181 GACAGGGTCT CACCCCACCC CAGATGCCTG GAGACCACAT GCACCCTTAT GGTGCCGAGC 241 CCCTTTTCCA TGACCTGGAT AGCGACGACA CCTCCCTCAG TAACCTGGGT GACTGTTTCC 301 TAGCAACCTC AGAAGCTGGG CCTCTGCAGT CCAGAGTGGG AAACCCCATT GACCATCTGT 361 ACTCCATGCA GAATTCTTAC TTCACATCTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATAAGC 541 ACAGCGCGGA TGTGGCCCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt