Construct: ORF TRCN0000469782
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013635.1_s317c1
- Derived from:
- ccsbBroadEn_13114
- DNA Barcode:
- CTCACGCGATGCTTAGCCGCCAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IZUMO2 (126123)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469782
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 126123 | IZUMO2 | IZUMO family member 2 | NM_152358.2 | 74.2% | 74.2% | 1_171del |
| 2 | human | 126123 | IZUMO2 | IZUMO family member 2 | XM_011526436.2 | 66.3% | 48.9% | 1_171del;495_496ins29;635_669del |
| 3 | human | 126123 | IZUMO2 | IZUMO family member 2 | XM_011526437.2 | 63.3% | 45.7% | (many diffs) |
| 4 | human | 126123 | IZUMO2 | IZUMO family member 2 | NM_001321449.1 | 61.8% | 63% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg catggagggg cctttcttcc gggactacgc gctgaacgtg tttgtgggga 121 aagtggagac aaatcaactg gaccttgtgg cgtcctttgt caagaaccaa acgcagcact 181 taatgggtaa ctctctgaaa gatgagcctc TGCTGGAAGA GCTGGTGACC CTCAGGGCGA 241 ATGTGATCAA GGAATTCAAG AAAGTTTTAA TTTCATATGA ATTAAAAGCC TGCAACCCCA 301 AACTTTGCCG CTTGCTAAAA GAAGAGGTGT TGGACTGTTT ACATTGCCAG AGGATCACTC 361 CCAAGTGTAT CCACAAAAAG TACTGCTTTG TCGACCGGCA ACCCCGCGTG GCCCTGCAGT 421 ACCAGATGGA CAGCAAATAC CCGAGGAACC AGGCGCTGTT GGGCATCCTC ATTTCTGTGT 481 CTCTGGCTGT CTTTGTCTTC GTGGTCATCG TGGTCTCGGC TTGTACATAC AGACAAAACC 541 GAAAACTCCT GCTGCAGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTCACGC GATGCTTAGC 721 CGCCAGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt