Construct: ORF TRCN0000469783
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011502.1_s317c1
- Derived from:
- ccsbBroadEn_06983
- DNA Barcode:
- GTGTCATAAGCGTCGATGCACCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNRPD1 (6632)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469783
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6632 | SNRPD1 | small nuclear ribonucleopro... | NM_006938.4 | 100% | 100% | |
| 2 | human | 6632 | SNRPD1 | small nuclear ribonucleopro... | NM_001291916.2 | 65.5% | 65.5% | 88_89ins123 |
| 3 | mouse | 20641 | Snrpd1 | small nuclear ribonucleopro... | NM_009226.4 | 94.3% | 100% | (many diffs) |
| 4 | mouse | 105244345 | LOC105244345 | small nuclear ribonucleopro... | XM_011239896.2 | 55% | 56.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 423
- ORF length:
- 357
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctcgtgaga tttttgatga aattgagtca tgaaactgta accattGAAT 121 TGAAGAACGG AACACAGGTC CATGGAACAA TCACAGGTGT GGATGTCAGC ATGAATACAC 181 ATCTTAAAGC TGTGAAAATG ACCCTGAAGA ACAGAGAACC TGTACAGCTG GAAACGCTGA 241 GTATTCGAGG AAATAACATT CGGTATTTTA TTCTACCAGA CAGTTTACCT CTGGATACAC 301 TACTTGTGGA TGTTGAACCT AAGGTGAAAT CTAAGAAAAG GGAAGCTGTT GCAGGAAGAG 361 GCAGAGGAAG AGGAAGAGGA AGAGGACGTG GCCGTGGCAG AGGAAGAGGG GGTCCTAGGC 421 GATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 541 GGCTTTATAT ATCTTGTGGA AAGGACGAGT GTCATAAGCG TCGATGCACC CTACGCGTTA 601 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt