Construct: ORF TRCN0000469793
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004786.1_s317c1
- Derived from:
- ccsbBroadEn_03663
- DNA Barcode:
- TATCGACAATACAATTAAAATGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP2R2D (55844)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469793
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55844 | PPP2R2D | protein phosphatase 2 regul... | NM_018461.5 | 100% | 100% | |
| 2 | human | 55844 | PPP2R2D | protein phosphatase 2 regul... | XM_006717914.3 | 80.4% | 79.6% | (many diffs) |
| 3 | human | 55844 | PPP2R2D | protein phosphatase 2 regul... | NM_001291310.2 | 63.5% | 63.5% | 0_1ins495 |
| 4 | human | 55844 | PPP2R2D | protein phosphatase 2 regul... | NR_033191.3 | 22.4% | (many diffs) | |
| 5 | human | 55844 | PPP2R2D | protein phosphatase 2 regul... | XR_002956987.1 | 21.4% | 0_1ins333;1027_4458del | |
| 6 | mouse | 52432 | Ppp2r2d | protein phosphatase 2, regu... | NM_026391.2 | 90% | 97.3% | (many diffs) |
| 7 | mouse | 52432 | Ppp2r2d | protein phosphatase 2, regu... | NM_001347618.1 | 56.2% | 61.5% | (many diffs) |
| 8 | mouse | 52432 | Ppp2r2d | protein phosphatase 2, regu... | XM_006508015.1 | 56.2% | 61.5% | (many diffs) |
| 9 | mouse | 52432 | Ppp2r2d | protein phosphatase 2, regu... | XM_006508016.1 | 56.2% | 61.5% | (many diffs) |
| 10 | mouse | 52432 | Ppp2r2d | protein phosphatase 2, regu... | XM_006508019.2 | 56.2% | 61.5% | (many diffs) |
| 11 | mouse | 52432 | Ppp2r2d | protein phosphatase 2, regu... | XM_017322350.1 | 56.2% | 61.5% | (many diffs) |
| 12 | mouse | 52432 | Ppp2r2d | protein phosphatase 2, regu... | XM_017322352.1 | 56.2% | 61.5% | (many diffs) |
| 13 | mouse | 52432 | Ppp2r2d | protein phosphatase 2, regu... | XM_006508020.3 | 55.5% | 60.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1425
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggagccgga ggcggcggct gccccgcggg cggcaacgac ttccagtggt 121 gcttctcgca ggtcaagggg gccatcgacg aggacgtggc cgaagcggac atcatttcca 181 ccgttgagtt taattactct ggagatcttc ttgcaacagg agacaagggc ggcagagttg 241 ttatttttca gcgtgaacaa gagaataaaa gccgccctca ttctagggga gaatataatg 301 tttacagcac ctttcaaagt catgaaccgg agtttgacta tttgaaaagt ctagaaattg 361 aggaaaaaat taataaaatt aggtggttac cacaacagaa tgctgctcat tttctactgt 421 ctacaaatga taaaactata aaattatgga aaataagtga acgggataaa agagcagaag 481 gttataacct gaaagacgaa gatggaagac ttcgagaccc atttaggatc acggcgctac 541 gggtcccaat attgaagccc atggatctta tggtagaagc gagtccacgg cgaatttttg 601 caaatgctca cacatatcat ataaattcca tttcagtaaa tagtgatcat gaaacatatc 661 tttctgcaga tgacctgaga attaatttat ggcacttaga aatcacagat agaagcttta 721 acatcgtgga catcaagcct gctaacatgg aggagctgac cgaagtcatc actgcagccg 781 agttccaccc gcaccagtgc aacgtgttcg tctacagcag tagcaaaggg accatccgcc 841 tgtgtgacat gcgctcctcg gccctgtgcg acagacactc caagtttttt gaagagcctg 901 aagatcccag cagtaggtcc ttcttctcag aaataatttc atccatatcc gatgtaaaat 961 tcagtcatag tgggcggtac atgatgacca gagactacct gtcggtgaag gtgtgggacc 1021 tcaacatgga gagcaggccg gtggagaccc accaggtcca cgagtacctg cgcagcaagc 1081 tcTGCTCTCT CTATGAGAAC GACTGCATCT TTGACAAGTT TGAGTGTTGC TGGAACGGTT 1141 CGGATAGCGC CATCATGACC GGGTCCTATA ACAACTTCTT CAGGATGTTT GATAGAGACA 1201 CGCGGAGGGA TGTGACCCTG GAGGCCTCGA GAGAGAGCAG CAAACCGCGC GCCAGCCTCA 1261 AACCCCGGAA GGTGTGTACG GGGGGTAAGC GGAGGAAAGA CGAGATCAGT GTGGACAGTC 1321 TGGACTTCAA CAAGAAGATC CTGCACACAG CCTGGCACCC CGTGGACAAT GTCATTGCCG 1381 TGGCTGCCAC CAATAACTTG TACATATTCC AGGACAAAAT CAACTACCCA ACTTTCTTGT 1441 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1501 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1561 GAAAGGACGA TATCGACAAT ACAATTAAAA TGTGACGCGT TAAGTCgaca atcaacctct 1621 ggattacaaa atttgtgaaa gatt