Construct: ORF TRCN0000469814
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008391.1_s317c1
- Derived from:
- BRDN0000395128
- DNA Barcode:
- AGCACATGAAACATATGTGAGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SBF2 (81846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469814
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 81846 | SBF2 | SET binding factor 2 | NM_030962.3 | 99.9% | 99.8% | (many diffs) |
2 | human | 81846 | SBF2 | SET binding factor 2 | XM_005253154.5 | 98.2% | 98.1% | (many diffs) |
3 | human | 81846 | SBF2 | SET binding factor 2 | XM_017018374.2 | 97.6% | 97.5% | (many diffs) |
4 | human | 81846 | SBF2 | SET binding factor 2 | XM_017018375.2 | 97.3% | 97.3% | (many diffs) |
5 | human | 81846 | SBF2 | SET binding factor 2 | XM_011520394.3 | 96.2% | 96.1% | (many diffs) |
6 | human | 81846 | SBF2 | SET binding factor 2 | XM_005253155.5 | 95.9% | 95.9% | (many diffs) |
7 | human | 81846 | SBF2 | SET binding factor 2 | XM_017018372.2 | 95.8% | 95.7% | 1_1delAins139;3508C>G;3654_3749del |
8 | human | 81846 | SBF2 | SET binding factor 2 | XM_017018373.2 | 95.8% | 95.7% | 1_1delAins139;3508C>G;3654_3749del |
9 | human | 81846 | SBF2 | SET binding factor 2 | XR_001747994.2 | 72.8% | (many diffs) | |
10 | human | 81846 | SBF2 | SET binding factor 2 | XM_011520395.3 | 68.3% | 68.2% | (many diffs) |
11 | human | 81846 | SBF2 | SET binding factor 2 | XM_017018376.2 | 68.3% | 68.2% | (many diffs) |
12 | human | 81846 | SBF2 | SET binding factor 2 | XM_011520396.3 | 62.2% | 62.3% | (many diffs) |
13 | human | 81846 | SBF2 | SET binding factor 2 | XM_017018377.2 | 59.6% | 59.4% | (many diffs) |
14 | mouse | 319934 | Sbf2 | SET binding factor 2 | XM_006507926.1 | 88.7% | 93.7% | (many diffs) |
15 | mouse | 319934 | Sbf2 | SET binding factor 2 | NM_177324.2 | 87.6% | 92.4% | (many diffs) |
16 | mouse | 319934 | Sbf2 | SET binding factor 2 | XM_006507925.1 | 87.3% | 92.1% | (many diffs) |
17 | mouse | 319934 | Sbf2 | SET binding factor 2 | XM_006507924.1 | 86.2% | 90.9% | (many diffs) |
18 | mouse | 319934 | Sbf2 | SET binding factor 2 | XM_006507927.2 | 86.1% | 90.9% | (many diffs) |
19 | mouse | 319934 | Sbf2 | SET binding factor 2 | XM_011241830.2 | 74.7% | 78.7% | (many diffs) |
20 | mouse | 319934 | Sbf2 | SET binding factor 2 | XR_001785572.1 | 68.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5616
- ORF length:
- 5547
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccggctg gcaggctact tcattgtggt aggctatgac cacgagaagc 121 caggatcagg agaaggtctg gggaaaataa tccagagatt tccacagaag gactgggatg 181 atacaccttt tccacaggga attgagttgt tttgtcagcc tggcgggtgg cagctgtcca 241 gagagaggaa gcagccaacg ttctttgtgg ttgtcctgac agacattgac tcagatcgac 301 attactgctc atgcctaacc ttctatgagg cagagatcaa tcttcaggga acaaagaagg 361 aagagattga aggtgaagca aaagtgtctg gtttaattca gcctgcagaa gtgtttgctc 421 ccaaaagcct ggtgttggta tccagattat attatccaga aatttttagg gcttgcctgg 481 gtttgatcta taccgtgtat gtggacagcc tgaatgtctc cttggaaagt ctaattgcaa 541 acctttgtgc ctgccttgtc ccagcggctg gagggtctca gaagctgttt tctttgggtg 601 caggagatag acagttgatc cagactcctt tacatgatag tcttcctatc acgggcacta 661 gtgtggctct cctgttccag caattgggaa ttcaaaatgt cctcagcctc ttttgtgcag 721 tcctcacaga aaataaggtt ctcttccatt ctgcaagttt ccagagactt agtgatgctt 781 gtagagccct ggaatcttta atgtttcctc ttaaatatag ttatccttat atccctattc 841 tcccggctca gctactggaa gttctaagtt ccccaacgcc tttcattatt ggagtacatt 901 ctgtctttaa aactgatgtc catgaacttt tagatgtaat catagcagat ttggatggag 961 gcactattaa aattcccgaa tgtattcacc tctcttccct cccagaacca cttctacatc 1021 agactcaatc agctctttct ttgattttac acccagattt ggaagtagca gatcatgctt 1081 ttcctcctcc acgaacagct ttatcccact caaaaatgct ggataaagag gtgcgagccg 1141 ttttccttag attatttgca caactcttcc aaggatatag atcctgcctg caacttataa 1201 gaattcatgc agagccagta atacatttcc acaagacagc attcttgggg cagcgtggtt 1261 tggtcgagaa tgatttcctc actaaagtac tcagtggaat ggcatttgca ggttttgttt 1321 cagaaagagg tcctccttat agatcttgtg atctctttga tgagttggta gcctttgaag 1381 tagagagaat taaagttgaa gaaaataacc cagtgaagat gataaagcat gtcagggaac 1441 ttgctgagca actattcaaa aatgagaatc caaatcctca tatggcattc cagaaagttc 1501 cacggccaac agaaggatcc catttgcgag ttcatattct tcctttccca gagattaatg 1561 aagcccgggt tcaggaatta atacaggaaa atgttgctaa gaaccagaat gcacctcctg 1621 ccacacgaat agaaaagaaa tgtgttgtgc cagcaggtcc acctgttgtt tcgataatgg 1681 acaaggtgac gacagttttc aacagtgcac aaagactaga agttgtcaga aactgtatct 1741 cattcatatt tgaaaataaa attttggaaa ctgaaaagac ccttcctgct gcactcagag 1801 cccttaaagg aaaggcagca agacagtgtc tcactgatga attgggtttg catgtccagc 1861 aaaaccgggc aatattagac catcaacagt ttgactacat aataaggatg atgaattgta 1921 ctctacagga ttgttcaagt ttagaagaat acaacattgc cgcagcatta ctccctttga 1981 ccagtgcttt ctataggaaa cttgcccctg gagtcagcca gtttgcttac acgtgtgtac 2041 aagaccaccc catttggaca aatcagcaat tttgggagac aaccttttac aatgcagtgc 2101 aggaacaggt tcgctccctt tatctctcag ccaaggaaga caatcatgcc ccgcatctga 2161 agcaaaagga taagcttcct gatgaccatt atcaggagaa gacagcaatg gacctggcag 2221 ctgagcaact acgcctttgg cctaccctga gcaagtcaac tcagcaagag ctagtgcaac 2281 atgaggaaag cactgtcttt agtcaggcca ttcactttgc aaacctcatg gtgaacctgc 2341 tagttccact cgacacaagt aaaaacaagc tcctaagaac atcagcgcca ggtgactggg 2401 agagcggaag caacagcatt gtcacaaaca gtattgcagg aagtgtagct gagagctatg 2461 atacagagag tgggtttgaa gattcagaga atactgacat tgccaattct gttgtgcggt 2521 tcattacccg atttattgac aaagtttgta cagagagtgg agttactcag gatcacatca 2581 agagccttca ttgcatgata ccaggaattg tagctatgca cattgagacc ctagaagcag 2641 tacatcgaga aagcagaaga cttccgccta ttcagaagcc caagattctt agacctgctc 2701 tgctgccagg agaagaaatt gtctgtgagg gtcttcgagt cttgctggat cctgatggaa 2761 gagaagaagc tactggaggt cttcttggag gccctcagct cctgccagca gaaggagcct 2821 tgttcctcac cacatacaga attctcttca gaggaacacc ccatgatcag ttagtgggtg 2881 agcagacagt tgtgcggagc tttcccattg cctccatcac caaggagaag aagattacaa 2941 tgcagaacca gctacagcag aacatgcaag aaggactgca gatcacatca gcatcttttc 3001 agttgattaa ggtagcattt gatgaagaag tcagtccaga agtagtagag atctttaaga 3061 aacagctgat gaagttccgt tatcctcagt ccattttcag tacctttgct tttgctgctg 3121 gacaaactac cccacaaata attttaccaa aacagaagga aaagaacact tcttttcgta 3181 ccttctcaaa aacaattgtg aaaggtgcca aaagggcagg gaaaatgaca attgggcggc 3241 aatatttact gaagaagaag acagggacaa ttgtggaaga aagagtaaat cgtcctggat 3301 ggaatgaaga tgatgatgta tctgtttcag atgagagtga gctccccaca agtaccaccc 3361 tgaaggcctc cgagaagtct acaatggaac agttggtgga aaaagcttgt ttcagagact 3421 atcagcgttt aggtttagga accataagtg gcagctcttc ccgttcaaga cccgagtatt 3481 ttagaattac tgcctccaac aggatgtatt cactctgccg gagctatcct ggccttttag 3541 tcgtacctca agctgtacag gacagtagtt taccaagagt agctcgctgc tatcgacaca 3601 atcgcctgcc tgttgtatgt tggaagaact caagaagtgg tactctgctc ctccgatctg 3661 gaggattcca tgggaaggga gtcgttggtc ttttcaaatc tcagaactcc cctgaggccg 3721 ctcctacctc ctctttagaa tcttccagta gcatagaaca agagaaatac ttgcaagcct 3781 tactgaatgc tgtttctgtc catcagaaac tcagaggcaa cagcactctt actgtcaggc 3841 cagcctttgc tctatctcca ggtgtgtggg caagtcttcg ctctagcact cgcttgatca 3901 gctctccaac atccttcatt gatgttggcg cccggctggc aggcaaggat cactcggcct 3961 ccttcagtaa cagcagctac ctacaaaacc agctcttgaa acggcaagca gccctttaca 4021 tatttggtga aaagtcgcaa ctaaggaact tcaaggtaga atttgcttta aattgtgagt 4081 ttgttcctgt tgaatttcat gaaatccggc aagtgaaagc cagttttaag aagctgatga 4141 gggcttgtat cccaagcacc atccctactg actcagaagt gaccttcctg aaagcgctgg 4201 gagattctga gtggttccca cagcttcaca ggataatgca gctggctgtg gttgtatcag 4261 aagtacttga gaatggttcc tcagttttgg tctgtttgga ggaaggctgg gacatcactg 4321 cacaagtgac atccctggtt cagttactca gtgatccctt ttataggaca cttgaaggct 4381 tccagatgtt ggttgaaaaa gagtggctct cttttggtca caaattcagt cagaggagca 4441 gcttgaccct caactgtcag gggagtggtt ttgctccagt cttcttacag ttcttagact 4501 gtgtacacca ggttcacaac cagtatccaa ctgagtttga attcaatctc tattacttaa 4561 agttcttggc tttccactat gtgtctaatc gctttaaaac atttctcctg gattcagact 4621 atgaaagatt agagcacgga actttatttg atgataaagg agaaaagcat gccaaaaaag 4681 gagtctgtat ttgggaatgt attgacagaa tgcacaagag gagtcccatt ttctttaatt 4741 atttatattc accattggaa atagaggctc taaagcccaa tgtaaacgtc tctagcctca 4801 agaagtggga ttactacata gaagagaccc tgtccacagg cccttcctat gactggatga 4861 tgctaacccc caagcacttc ccctccgaag actctgacct ggctggagaa gctgggccac 4921 ggagccagag gagaacagtg tggccatgct atgatgatgt cagctgtact cagcctgatg 4981 ctctcaccag ccttttcagt gaaattgaaa aattggagca caaattgaac caagcccctg 5041 agaagtggca gcagctgtgg gaaagggtaa ccgtggacct taaagaagaa ccaagaacag 5101 atcgctccca aagacacctg tcgagatccc caggaattgt gtctaccaac ctaccttcct 5161 atcagaagag gtctctgcta catcTCCCAG ACAGCAGCAT GGGGGAGGAA CAGAATTCCA 5221 GCATCTCCCC ATCCAATGGA GTGGAGCGAA GAGCAGCCAC GCTCTATAGC CAGTATACAT 5281 CCAAGAATGA TGAAAACAGG TCCTTTGAGG GAACACTTTA TAAAAGAGGG GCTTTGCTGA 5341 AAGGTTGGAA GCCCCGTTGG TTTGTTTTGG ATGTAACAAA ACATCAGCTG CGCTACTATG 5401 ACTCAGGTGA GGACACAAGC TGTAAAGGCC ACATTGATCT GGCTGAAGTA GAAATGGTCA 5461 TCCCTGCTGG CCCCAGCATG GGAGCCCCAA AGCACACAAG TGACAAGGCT TTCTTTGATC 5521 TCAAGACCAG CAAACGTGTG TATAACTTCT GCGCCCAGGA TGGACAGAGT GCCCAGCAAT 5581 GGATGGACAA GATCCAGAGT TGTATCTCTG ATGCCTTGCC AACTTTCTTG TACAAAGTGG 5641 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 5701 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 5761 AAGCACATGA AACATATGTG AGCCGACGCG TTAAGTCgac aatcaacctc tggattacaa 5821 aatttgtgaa agatt