Construct: ORF TRCN0000469817
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015411.1_s317c1
- Derived from:
- ccsbBroadEn_10191
- DNA Barcode:
- ACCTCCTTCAGGTTCAAAACTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FOXD4L6 (653404)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469817
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653404 | FOXD4L6 | forkhead box D4 like 6 | NM_001085476.1 | 99.9% | 99.7% | 1247C>G |
| 2 | human | 286380 | FOXD4L3 | forkhead box D4 like 3 | NM_199135.4 | 99.7% | 99.2% | 695G>A;1030A>G;1247C>G |
| 3 | human | 653427 | FOXD4L5 | forkhead box D4 like 5 | NM_001126334.1 | 98.8% | 98% | (many diffs) |
| 4 | human | 349334 | FOXD4L4 | forkhead box D4 like 4 | NM_199244.3 | 98.8% | 98% | (many diffs) |
| 5 | human | 200350 | FOXD4L1 | forkhead box D4 like 1 | NM_012184.4 | 91.9% | 67.9% | (many diffs) |
| 6 | human | 2298 | FOXD4 | forkhead box D4 | NM_207305.4 | 84.2% | 78% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1320
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacctgcca agagctgagc gccttcgctc cacaccgcag cgcagcctcc 121 gggactccga tggggaagac ggtaaaatcg atgtcctggg agaggaggaa gatgaagacg 181 aggtggaaga cgaggaggag gcggcgagcc agcagttcct agagcagtcg ctccagccgg 241 ggctgcaggt ggcccggtgg ggcggggttg cgcttccccg agagcacatc gagggcggcg 301 gcggcccgag cgacccctca gagtttggca ccaagttcag ggcaccgcca aggtctgcgg 361 cggcctctga agatgcccgg cagccggcaa agccccccta ctcgtacatc gcgctcatca 421 ccatggccat cctgcaaaac ccgcacaagc gcctcacgct cagcggcatc tgcgccttca 481 ttagtggccg cttcccctac taccgccgca agttccccgc ctggcagaac agcatccgcc 541 acaacctctc gctgaacgac tgcttcgtta agatcccccg cgagccgggc cacccaggca 601 agggcaacta ctggagcctg gaccccgcct cccaagacat gttcgacaat ggcagctttc 661 tccggcgtag gaagcgtttc aagcgccacc aactgacccc gggagcccac ctgccccacc 721 ccttccctct acctgctgca cacgccgccc tgcacaaccc ccacccaggc cctctgcttg 781 gggcccctgc cccgccgcag ccagtcccgg gggcctaccc caacaccgcc cccgggagac 841 gcccttacgc tctgctgcac ccgcatcctc ttcgctacct actgctctcg gcccccgtct 901 atgccggggc accgaagaaa gcagaaggcg cggccctggc gaccccggca cccttcccgt 961 gctgcagccc tcacttggtc cTCAGCCTTG GGAGGAGGGC AAGGGTCTGG CGTCGCCACC 1021 GGGAGGCGGA TGCATCTCTT TCAGCATTGA GAGTATTATG CAAGGGGTCA GGGGAGCGGG 1081 TACAGGGGCT GCGCAGAGTT TGTCCCCGAC CGCGTGGAGC TACTGCCACC TGCTCCAGCG 1141 ACCATCAAGC CTGTTGCATC CCCAGACCGC TGCCCCTTTG CTGCAAGTGT CCGCCGCCGC 1201 CGCTGCTCGG ACAATTTTGC AGCAATAGCA GCAGCATCAG GAGGAGGACT GCGCCAACGG 1261 CTGCGCTCCC ACCAAGGGCG CGGTGCTGGG CGGGCACCTG TCGGCCTCGT CGGCGCTGCT 1321 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGAACCTC CTTCAGGTTC AAAACTAGCA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt