Transcript: Human NM_199135.4

Homo sapiens forkhead box D4 like 3 (FOXD4L3), mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
FOXD4L3 (286380)
Length:
2218
CDS:
86..1339

Additional Resources:

NCBI RefSeq record:
NM_199135.4
NBCI Gene record:
FOXD4L3 (286380)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_199135.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000262818 ACTACACTACATCGTTTATTT pLKO_005 2031 3UTR 100% 15.000 7.500 Y FOXD4L6 n/a
2 TRCN0000255491 TTTCAGCATTGAGAGTATTAT pLKO_005 1056 CDS 100% 15.000 7.500 Y FOXD4L4 n/a
3 TRCN0000107795 CGTGCCATCTTTAATGTTAAA pLKO.1 1957 3UTR 100% 13.200 6.600 Y FOXD4L3 n/a
4 TRCN0000021031 CTTTCAGCATTGAGAGTATTA pLKO.1 1055 CDS 100% 13.200 6.600 Y FOXD4L4 n/a
5 TRCN0000107796 TCTTTCAGCATTGAGAGTATT pLKO.1 1054 CDS 100% 13.200 6.600 Y FOXD4L3 n/a
6 TRCN0000262397 TTCAGCATTGAGAGTATTATG pLKO_005 1057 CDS 100% 13.200 6.600 Y FOXD4L5 n/a
7 TRCN0000422982 AGAAAGAAACAGCTGGATTAC pLKO_005 1594 3UTR 100% 10.800 5.400 Y FOXD4L1 n/a
8 TRCN0000262817 CCCAAGACATGTTCGACAATG pLKO_005 648 CDS 100% 10.800 5.400 Y FOXD4L6 n/a
9 TRCN0000107900 GCCTAGTTATATAGACGAAAT pLKO.1 1997 3UTR 100% 10.800 5.400 Y FOXD4L3 n/a
10 TRCN0000262816 GATGCATCTCTTTCAGCATTG pLKO_005 1046 CDS 100% 6.000 3.000 Y FOXD4L6 n/a
11 TRCN0000255490 ACTCGTACATCGCGCTCATCA pLKO_005 417 CDS 100% 4.950 2.475 Y FOXD4L4 n/a
12 TRCN0000107797 CTCAGAGTTTGGCACCAAGTT pLKO.1 334 CDS 100% 4.950 2.475 Y FOXD4L3 n/a
13 TRCN0000017866 CTCTTTCAGCATTGAGAGTAT pLKO.1 1053 CDS 100% 4.950 2.475 Y FOXD4 n/a
14 TRCN0000174049 CTCTTTCAGCATTGAGAGTAT pLKO.1 1053 CDS 100% 4.950 2.475 Y FOXD4 n/a
15 TRCN0000107901 TCCCAAGACATGTTCGACAAT pLKO.1 647 CDS 100% 4.950 2.475 Y FOXD4L3 n/a
16 TRCN0000418966 CATCCTCTTCGCTACCTACTG pLKO_005 881 CDS 100% 4.050 2.025 Y FOXD4L4 n/a
17 TRCN0000107902 CTACTCGTACATCGCGCTCAT pLKO.1 415 CDS 100% 4.050 2.025 Y FOXD4L3 n/a
18 TRCN0000282437 GCATCCTCTTCGCTACCTACT pLKO_005 880 CDS 100% 4.050 2.025 Y FOXD4L6 n/a
19 TRCN0000262395 TACTCGTACATCGCGCTCATC pLKO_005 416 CDS 100% 4.050 2.025 Y FOXD4L5 n/a
20 TRCN0000262819 TTTCAAGCGCCACCAACTGAC pLKO_005 694 CDS 100% 4.050 2.025 Y FOXD4L6 n/a
21 TRCN0000107903 CGACCATCAAGCCTGTTGCAT pLKO.1 1156 CDS 100% 3.000 1.500 Y FOXD4L3 n/a
22 TRCN0000107904 CATGTTCGACAATGGCAGCTT pLKO.1 655 CDS 100% 2.640 1.320 Y FOXD4L3 n/a
23 TRCN0000016763 CGGATGCATCTCTTTCAGCAT pLKO.1 1044 CDS 100% 2.640 1.320 Y FOXD4L1 n/a
24 TRCN0000016765 GAAGATGAAGACGAGGTGGAA pLKO.1 185 CDS 100% 2.640 1.320 Y FOXD4L1 n/a
25 TRCN0000016766 CCGGCGTAGGAAGCGTTTCAA pLKO.1 679 CDS 100% 1.875 0.938 Y FOXD4L1 n/a
26 TRCN0000021032 CCAGCGACCATCAAGCCTGTT pLKO.1 1152 CDS 100% 1.350 0.675 Y FOXD4L4 n/a
27 TRCN0000017867 GCAGAACAGCATCCGCCACAA pLKO.1 541 CDS 100% 1.350 0.675 Y FOXD4 n/a
28 TRCN0000021030 GCTGAACGACTGCTTCGTTAA pLKO.1 568 CDS 100% 1.080 0.540 Y FOXD4L4 n/a
29 TRCN0000107798 CCGCATCCTCTTCGCTACCTA pLKO.1 878 CDS 100% 1.000 0.500 Y FOXD4L3 n/a
30 TRCN0000016764 CGCCTTCATTAGTGGCCGCTT pLKO.1 490 CDS 100% 0.720 0.360 Y FOXD4L1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_199135.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10191 pDONR223 100% 99.7% 99.2% None 695G>A;1030A>G;1247C>G n/a
2 ccsbBroad304_10191 pLX_304 0% 99.7% 99.2% V5 695G>A;1030A>G;1247C>G n/a
3 TRCN0000469817 ACCTCCTTCAGGTTCAAAACTAGC pLX_317 27.3% 99.7% 99.2% V5 695G>A;1030A>G;1247C>G n/a
4 ccsbBroadEn_00574 pDONR223 100% 84.2% 78% None (many diffs) n/a
5 ccsbBroad304_00574 pLX_304 0% 84.2% 78% V5 (many diffs) n/a
6 TRCN0000476871 CCCACCCTAACGAGCTCGAATCCA pLX_317 25.3% 84.2% 78% V5 (many diffs) n/a
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