Construct: ORF TRCN0000469857

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010745.1_s317c1
Derived from:
ccsbBroadEn_02212
DNA Barcode:
AGATTTCGTTCTTTTGATTCATAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNF7 (9616)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469857
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9616 RNF7 ring finger protein 7 NM_014245.5 100% 100%
2 human 9616 RNF7 ring finger protein 7 NM_001201370.1 85.8% 85.8% 174_175ins48
3 human 9616 RNF7 ring finger protein 7 NM_183237.2 79.6% 51.5% 167_168insGGTGATG;270_271ins62
4 human 9616 RNF7 ring finger protein 7 NR_037702.1 14.9% 1_133del;309_572del;737_2274del
5 human 9616 RNF7 ring finger protein 7 NR_037703.1 14.8% (many diffs)
6 mouse 19823 Rnf7 ring finger protein 7 NM_011279.3 93.8% 96.4% (many diffs)
7 mouse 19823 Rnf7 ring finger protein 7 NM_001311135.1 74% 45.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
405
ORF length:
339
Sequence:
1tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG
61TTGGCATGGC CGACGTGGAA GACGGAGAGG AAACCTGCGC CCTGGCCTCT CACTCCGGGA
121GCTCAGGCTC CAAGTCGGGA GGCGACAAGA TGTTCTCCCT CAAGAAGTGG AACGCGGTGG
181CCATGTGGAG CTGGGACGTG GAGTGCGATA CGTGCGCCAT CTGCAGGGTC CAGGTGATGG
241ATGCCTGTCT TAGATGTCAA GCTGAAAACA AACAAGAGGA CTGTGTTGTG GTCTGGGGAG
301AATGTAATCA TTCCTTCCAC AACTGCTGCA TGTCCCTGTG GGTGAAACAG AACAATCGCT
361GCCCTCTCTG CCAGCAGGAC TGGGTGGTCC AAAGAATCGG CAAATGCCCA ACTTTCTTGT
421ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
481AGTAATGAAC TAGCTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT
541TGTGGAAAGG ACGAAGATTT CGTTCTTTTG ATTCATAAAC GCGTTAAGTC gacaatcaac
601ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)