Construct: ORF TRCN0000469870

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008281.1_s317c1
Derived from:
ccsbBroadEn_09303
DNA Barcode:
ATGCAGCGACCTAGGTCATCTGGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LRSAM1 (90678)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469870
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 90678 LRSAM1 leucine rich repeat and ste... NM_001005373.3 99.9% 99.8% 249C>T;952A>G
2 human 90678 LRSAM1 leucine rich repeat and ste... NM_001005374.3 99.9% 99.8% 249C>T;952A>G
3 human 90678 LRSAM1 leucine rich repeat and ste... NM_138361.5 99.9% 99.8% 249C>T;952A>G
4 human 90678 LRSAM1 leucine rich repeat and ste... XM_017015283.1 99.9% 99.8% 249C>T;952A>G
5 human 90678 LRSAM1 leucine rich repeat and ste... NM_001190723.3 96.1% 96.1% 249C>T;952A>G;1418_1419ins81
6 human 90678 LRSAM1 leucine rich repeat and ste... XM_006717316.4 95.3% 95.2% 249C>T;952A>G;1599_1600ins99
7 human 90678 LRSAM1 leucine rich repeat and ste... XR_929874.3 69.1% (many diffs)
8 human 90678 LRSAM1 leucine rich repeat and ste... XR_001746415.2 65.5% (many diffs)
9 human 90678 LRSAM1 leucine rich repeat and ste... XM_017015284.2 63.5% 63.4% 0_1ins789;163A>G
10 mouse 227738 Lrsam1 leucine rich repeat and ste... NM_199302.2 85.7% 87.6% (many diffs)
11 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497969.3 85.7% 87.6% (many diffs)
12 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497970.3 85.7% 87.6% (many diffs)
13 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497971.3 85.7% 87.6% (many diffs)
14 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497972.3 85.7% 87.6% (many diffs)
15 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497962.3 82.2% 84% (many diffs)
16 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497963.3 82.2% 84% (many diffs)
17 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497964.3 82.2% 84% (many diffs)
18 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497965.3 82.2% 84% (many diffs)
19 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497966.3 82.2% 84% (many diffs)
20 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497967.3 79.8% 81.5% (many diffs)
21 mouse 227738 Lrsam1 leucine rich repeat and ste... XM_006497968.3 78.9% 80.7% (many diffs)
22 mouse 227738 Lrsam1 leucine rich repeat and ste... XR_374086.2 54.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2235
ORF length:
2169
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc gctcttcttc cggaagcgga aacccagtga ggaggctcgg aaacgcctgg
121agtaccagat gtgtttggca aaagaagctg gggcagatga cattctcgac atctctaaat
181gtgagctctc agagattcca tttggagctt ttgcaacatg caaagttctg cagaagaagg
241tgctgatcgt ccacacgaat cacctcactt ccctgcttcc caaatcctgc agcctcctga
301gtctggcaac cattaaggtt ctagatctcc acgataatca gctgacagcc cttcctgacg
361atctggggca gctgactgcc ctccaggtct taaacgtgga aaggaatcaa ctgatgcagc
421tcccacgttc cattgggaac ctgacccagc tccagactct caatgttaaa gacaacaagc
481tgaaggagct tccagacacc gtgggggagc ttcgaagcct gcgtaccctc aacatcagtg
541gaaacgagat ccagagattg ccgcagatgc tggctcacgt tcgaaccctg gagatgctga
601gccttgacgc ctcggccatg gtctacccgc cgcgggaggt gtgtggtgcc ggcactgcgg
661ccatcttgca gttcctctgc aaagagtcag ggctggaata ctacccccct tctcagtact
721tgctgccaat tctggagcaa gatggaatcg agaactctcg ggacagccct gatgggccca
781cggacagatt ctcaagggag gagttagagt ggcagaacag gttctcagac tatgagaaga
841ggaaggaaca gaagatgctg gagaaactcg agtttgaacg gcgcctggaa ctggggcagc
901gggagcacac ccagctcctt cagcagagca gcagccagaa ggatgagatc cttcagacgg
961tcaaggagga gcagtcccgg ctggagcagg gcctgagtga gcaccagcgc cacctcgacg
1021cagagcggca gcggctgcag gagcagctga agcagacgga acagaacatt tccagccgga
1081tccagaagct gctgcaggac aatcagagac aaaagaaaag ctccgagatt ttgaaatcgc
1141tggaaaatga aagaataaga atggaacagt tgatgtccat aacccaggag gagactgaga
1201gcctgcggcg acgtgacgtt gcctccgcca tgcagcagat gctgactgag agctgtaaga
1261accggctcat ccagatggcc tacgaatctc agaggcagaa cttggtccag caggcctgtt
1321ccagcatggc cgaaatggat gaacgattcc agcagattct gtcgtggcag caaatggatc
1381agaacaaagc catcagccag atcctgcagg agagcgcgat gcagaaggct gcgttcgagg
1441cactccaggt gaagaaagac ctgatgcatc ggcagatcag gagccagatt aagttaatag
1501aaactgagtt attgcagctg acacagctgg agttaaagag gaagtccctg gacacagagt
1561cactccagga gatgatctcg gagcagcgct gggccctcag ctccctgctc cagcagctgc
1621tcaaagagaa gcagcagcga gaggaagagc tccgggaaat cctgacggag ttagaagcca
1681aaagtgaaac caggcaggaa aattactggc tgattcagta tcaacggctt ttgaaccaga
1741agcccttgtc cttgaagctg caagaagagg ggatggagcg ccagctggtg gccctcctgg
1801aggagctgtc ggctgagcac tacctgccca tctttgcgca ccaccgcctc tcactggacc
1861tgcTGAGCCA AATGAGCCCA GGGGACCTGG CCAAGGTGGG CGTCTCAGAA GCTGGCCTGC
1921AGCACGAGAT CCTCCGGAGA GTCCAGGAAC TGCTGGATGC AGCCAGGATC CAGCCAGAGC
1981TGAAACCACC AATGGGTGAG GTCGTCACCC CTACGGCCCC CCAGGAGCCT CCTGAGTCTG
2041TGAGGCCATC CGCTCCCCCT GCAGAGCTGG AGGTGCAGGC CTCAGAGTGT GTCGTGTGCC
2101TGGAACGGGA GGCCCAGATG ATCTTCCTCA ACTGTGGCCA CGTCTGCTGC TGCCAGCAGT
2161GCTGCCAGCC ACTGCGCACC TGCCCGCTGT GCCGCCAGGA CATCGCCCAG CGCCTCCGCA
2221TCTACCACAG CAGCTCCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2281CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2341TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATGCAGCGAC CTAGGTCATC
2401TGGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)