Construct: ORF TRCN0000469884
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005922.1_s317c1
- Derived from:
- ccsbBroadEn_00408
- DNA Barcode:
- CCCTTCGTGCCAACCAGCCCTGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP2C9 (1559)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469884
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1559 | CYP2C9 | cytochrome P450 family 2 su... | NM_000771.4 | 100% | 100% | |
| 2 | human | 1557 | CYP2C19 | cytochrome P450 family 2 su... | NM_000769.4 | 94.8% | 91.4% | (many diffs) |
| 3 | human | 1562 | CYP2C18 | cytochrome P450 family 2 su... | NM_000772.3 | 87.7% | 81.8% | (many diffs) |
| 4 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_000770.3 | 84.7% | 77.9% | (many diffs) |
| 5 | human | 1562 | CYP2C18 | cytochrome P450 family 2 su... | NM_001128925.2 | 78.5% | 74% | (many diffs) |
| 6 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_001198853.1 | 72.5% | 67.1% | (many diffs) |
| 7 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_001198855.1 | 72.5% | 67.1% | (many diffs) |
| 8 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_001198854.1 | 66.4% | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1539
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggattctctt gtggtccttg tgctctgtct ctcatgtttg cttctccttt 121 cactctggag acagagctct gggagaggaa aactccctcc tggccccact cctctcccag 181 tgattggaaa tatcctacag ataggtatta aggacatcag caaatcctta accaatctct 241 caaaggtcta tggccctgtg ttcactctgt attttggcct gaaacccata gtggtgctgc 301 atggatatga agcagtgaag gaagccctga ttgatcttgg agaggagttt tctggaagag 361 gcattttccc actggctgaa agagctaaca gaggatttgg aattgttttc agcaatggaa 421 agaaatggaa ggagatccgg cgtttctccc tcatgacgct gcggaatttt gggatgggga 481 agaggagcat tgaggaccgt gttcaagagg aagcccgctg ccttgtggag gagttgagaa 541 aaaccaaggc ctcaccctgt gatcccactt tcatcctggg ctgtgctccc tgcaatgtga 601 tctgctccat tattttccat aaacgttttg attataaaga tcagcaattt cttaacttaa 661 tggaaaagtt gaatgaaaac atcaagattt tgagcagccc ctggatccag atctgcaata 721 atttttctcc tatcattgat tacttcccgg gaactcacaa caaattactt aaaaacgttg 781 cttttatgaa aagttatatt ttggaaaaag taaaagaaca ccaagaatca atggacatga 841 acaaccctca ggactttatt gattgcttcc tgatgaaaat ggagaaggaa aagcacaacc 901 aaccatctga atttactatt gaaagcttgg aaaacactgc agttgacttg tttggagctg 961 ggacagagac gacaagcaca accctgagat atgctctcct tctcctgctg aagcacccag 1021 aggtcacagc taaagtccag gaagagattg aacgtgtgat tggcagaaac cggagcccct 1081 gcatgcaaga caggagccac atgccctaca cagatgctgt ggtGCACGAG GTCCAGAGAT 1141 ACATTGACCT TCTCCCCACC AGCCTGCCCC ATGCAGTGAC CTGTGACATT AAATTCAGAA 1201 ACTATCTCAT TCCCAAGGGC ACAACCATAT TAATTTCCCT GACTTCTGTG CTACATGACA 1261 ACAAAGAATT TCCCAACCCA GAGATGTTTG ACCCTCATCA CTTTCTGGAT GAAGGTGGCA 1321 ATTTTAAGAA AAGTAAATAC TTCATGCCTT TCTCAGCAGG AAAACGGATT TGTGTGGGAG 1381 AAGCCCTGGC CGGCATGGAG CTGTTTTTAT TCCTGACCTC CATTTTACAG AACTTTAACC 1441 TGAAATCTCT GGTTGACCCA AAGAACCTTG ACACCACTCC AGTTGTCAAT GGATTTGCCT 1501 CTGTGCCGCC CTTCTACCAG CTGTGCTTCA TTCCTGTCTT GCCAACTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGCCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGACCCTTC GTGCCAACCA GCCCTGGAAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt