Transcript: Human NM_001198855.1

Homo sapiens cytochrome P450 family 2 subfamily C member 8 (CYP2C8), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
CYP2C8 (1558)
Length:
2024
CDS:
406..1668

Additional Resources:

NCBI RefSeq record:
NM_001198855.1
NBCI Gene record:
CYP2C8 (1558)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001198855.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000235064 TGGCACTGTAGCTGATCTATT pLKO_005 1059 CDS 100% 13.200 18.480 N CYP2C8 n/a
2 TRCN0000235062 GTTGCTCTTACACGAAGTTAC pLKO_005 904 CDS 100% 10.800 15.120 N CYP2C8 n/a
3 TRCN0000064072 GCAGTTACCAAAGGGATTGTT pLKO.1 1606 CDS 100% 5.625 7.875 N CYP2C8 n/a
4 TRCN0000064069 CCACTGATACTAAGTTCAGAA pLKO.1 1307 CDS 100% 4.950 6.930 N CYP2C8 n/a
5 TRCN0000235063 CCAAGCATCACTGGATGTTAA pLKO_005 948 CDS 100% 13.200 10.560 N CYP2C8 n/a
6 TRCN0000235065 CAAGGACATTCCCACTATTAT pLKO_005 1724 3UTR 100% 15.000 10.500 N CYP2C8 n/a
7 TRCN0000235061 CCTCACAACCTTGCGGAATTT pLKO_005 576 CDS 100% 13.200 9.240 N CYP2C8 n/a
8 TRCN0000064070 CCTCTTCCTATTATTGGAAAT pLKO.1 198 5UTR 100% 10.800 7.560 N CYP2C8 n/a
9 TRCN0000064071 CGAAGTTACATTAGGGAGAAA pLKO.1 916 CDS 100% 4.950 3.465 N CYP2C8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001198855.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00409 pDONR223 100% 72.5% 66.3% None (many diffs) n/a
2 ccsbBroad304_00409 pLX_304 0% 72.5% 66.3% V5 (many diffs) n/a
3 TRCN0000480849 TTGTGTCTTGCGGCTCGGGATGGG pLX_317 26.8% 72.5% 66.3% V5 (many diffs) n/a
4 ccsbBroadEn_00408 pDONR223 100% 72.5% 67.1% None (many diffs) n/a
5 ccsbBroad304_00408 pLX_304 0% 72.5% 67.1% V5 (many diffs) n/a
6 TRCN0000469884 CCCTTCGTGCCAACCAGCCCTGGA pLX_317 28.5% 72.5% 67.1% V5 (many diffs) n/a
7 ccsbBroadEn_10763 pDONR223 100% 16.6% 14.6% None (many diffs) n/a
8 ccsbBroad304_10763 pLX_304 0% 16.6% 14.6% V5 (many diffs) n/a
9 TRCN0000475303 TGCAACCTTACTCCTTTTATACCA pLX_317 67.3% 16.6% 14.6% V5 (many diffs) n/a
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