Construct: ORF TRCN0000469900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001364.1_s317c1
- Derived from:
- ccsbBroadEn_06965
- DNA Barcode:
- GCAACCCAAGTTAATCTTAAGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC12A4 (6560)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6560 | SLC12A4 | solute carrier family 12 me... | NM_005072.5 | 99.9% | 100% | 2881C>A |
| 2 | human | 6560 | SLC12A4 | solute carrier family 12 me... | NM_001145961.2 | 99.4% | 99.4% | 2720_2721insGGTGGAGGTGGTGGAGAT;2863C>A |
| 3 | human | 6560 | SLC12A4 | solute carrier family 12 me... | NM_001145963.2 | 97.8% | 96.6% | (many diffs) |
| 4 | human | 6560 | SLC12A4 | solute carrier family 12 me... | NM_001145964.2 | 96.9% | 96.5% | (many diffs) |
| 5 | human | 6560 | SLC12A4 | solute carrier family 12 me... | NM_001145962.1 | 94.1% | 93.4% | 1_101del;215_216ins95;2887C>A |
| 6 | mouse | 20498 | Slc12a4 | solute carrier family 12, m... | NM_009195.3 | 89.2% | 96.2% | (many diffs) |
| 7 | mouse | 20498 | Slc12a4 | solute carrier family 12, m... | NM_001253804.1 | 89% | 96% | (many diffs) |
| 8 | mouse | 20498 | Slc12a4 | solute carrier family 12, m... | XM_006530792.2 | 84.6% | 90% | (many diffs) |
| 9 | mouse | 20498 | Slc12a4 | solute carrier family 12, m... | NR_045594.1 | 75.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3321
- ORF length:
- 3255
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tcacttcacc gtggtgccag tggacgggcc gaggcgcggc gactatgaca 121 acctcgaggg gctcagttgg gtggactacg gggagcgcgc cgagctggat gactcggacg 181 gacatggcaa ccacagagag agcagccctt ttctttcccc cttggaggct tccagaggaa 241 ttgactacta tgacaggaac ctggcactgt ttgaggaaga gctggacatc cgcccaaagg 301 tatcgtctct tctgggaaag ctcgtcagct acaccaacct cacccagggc gccaaagagc 361 atgaggaggc cgagagtggg gagggcaccc gccggagggc agccgaggca cccagcatgg 421 gcaccctcat gggggtgtac ctgccctgcc tgcagaatat ctttggggtt atcctcttcc 481 tgcggctgac ctggatggtg ggcacagcag gtgtgctaca ggccctcctc atcgtgctta 541 tctgctgctg ttgtaccctg ctgacggcca tctccatgag tgccatcgcc accaacggtg 601 tggttccagc tgggggctcc tatttcatga tctctcgttc actggggcca gaatttggag 661 gtgctgtggg cctgtgcttc tacctgggaa caacattcgc agcagccatg tacatcctgg 721 gggccatcga gatcttgctg acctacattg ccccaccagc tgccattttt tacccatcgg 781 gtgctcatga cacgtcgaat gccactttga acaatatgcg tgtgtatggg accattttcc 841 tgaccttcat gaccctggtg gtgtttgtgg gggtcaagta tgtgaacaaa tttgcctcgc 901 tcttcctggc ctgtgtgatc atctccatcc tctccatcta tgctgggggc ataaagtcta 961 tatttgaccc tcccgtgttt ccggtatgca tgctgggcaa caggaccctg tcccgggacc 1021 agtttgacat ctgtgccaag acagctgtag tggacaatga gacagtggcc acccagctat 1081 ggagtttctt ctgccacagc cccaacctta cgaccgactc ctgtgacccc tacttcatgc 1141 tcaacaatgt gaccgagatc cctggcatcc ccggggcagc tgctggtgtg ctccaggaaa 1201 acctgtggag cgcctacctg gagaagggtg acatcgtgga gaagcatggg ctgccctccg 1261 cagatgcccc gagcctgaag gagagcctgc ctctgtacgt ggtcgctgac atcgccacat 1321 ccttcaccgt gctggtcggc atcttcttcc cttctgtaac aggcatcatg gctggctcaa 1381 accgctctgg ggaccttcgt gacgcccaga agtctatccc tgtggggacc attctggcca 1441 tcattacaac ttccctcgtg tacttcagca gtgtggttct ctttggtgcc tgcattgagg 1501 gtgtggttct ccgggacaag tatggcgatg gtgtcagcag gaacttggtg gtgggcacac 1561 tggcctggcc ttcaccctgg gtcatcgtca tcggctcctt cttttcaacg tgtggcgctg 1621 gcctccagag cctcacaggg gcaccacgcc tattgcaggc cattgccaag gacaacatca 1681 tccccttcct ccgggtgttt ggccacggga aggtgaatgg tgaacccaca tgggcactcc 1741 tcctgacggc actcatcgcc gagctgggca tcctcatcgc ctccctcgac atggtggccc 1801 ccatcttatc catgttcttt ctgatgtgct acctgttcgt gaacctcgcc tgtgcggtgc 1861 agacactcct gaggaccccc aactggcggc cccggttcaa gtactatcac tgggcgctgt 1921 ccttcctggg catgagtctc tgcctggccc ttatgtttgt ctcctcctgg tactatgccc 1981 tggtggccat gctcatcgcc ggcatgatct acaaatacat cgagtaccaa ggggctgaga 2041 aggagtgggg tgacgggatc cgaggcctgt ccctgagcgc tgcccgctac gcgctgttgc 2101 ggctggagga ggggcctcct cacaccaaga actggcggcc gcagctgctg gtgctgctga 2161 agctggacga ggacctccac gtgaagtacc cgcggctcct caccttcgcc tcccagctca 2221 aggctggcaa gggcctgacc attgttggtt ctgtcatcca ggggagcttc ttggagagct 2281 atggcgaggc tcaggccgcc gagcagacca tcaagaacat gatggaaatt gagaaggtga 2341 agggcttctg ccaggtggtg gtggccagca aggtgcggga ggggctggcc cacctcatcc 2401 agtcctgtgg cctgggaggc atgcggcata actccgtggt gctgggctgg ccctacggct 2461 ggcgacagag cgaggacccc cgtgcctgga agaccttcat tgacaccgtg cgctgcacta 2521 cggctgccca cctggccctg ctcgtgccca agaacatcgc cttctacccc agcaaccacg 2581 agcgctacct ggagggccac atagacgtgt ggtggatcgt gcacgatggt ggcatgctca 2641 tgcttctgcc cttcctgctg cgccagcata aggtctggag gaagtgccgg atgcgcatct 2701 tcacagtggc ccagatggat gacaacagca tccagatgaa gaaggacctg gctgtctttc 2761 tgtaccatct gcgccttgag gccgaggtgg aggtggtgga gatgcataac agtgacatct 2821 ctgcatacac ctacgagcgg acgctgatga tggagcagcg gtcgcagatg ctgcggcaga 2881 tgagactgac caagactgag cgggagcgag aagcccagct ggtcaaggat cggcacTCGG 2941 CCCTGAGGCT GGAGAGCCTG TACTCGGACG AGGAAGATGA GTCTGCAGTG GGGGCTGACA 3001 AGATCCAGAT GACGTGGACC AGGGACAAGT ACATGACTGA GACCTGGGAC CCCAGCCATG 3061 CCCCTGACAA TTTCCGGGAG CTGGTGCACA TTAAGCCGGA CCAATCCAAT GTGCGGCGCA 3121 TGCACACTGC TGTGAAGCTC AATGAAGTCA TTGTCACGCG CTCCCACGAC GCCCGCCTGG 3181 TTCTCCTAAA CATGCCTGGC CCACCCAGGA ACAGTGAGGG CGACGAGAAC TACATGGAGT 3241 TCCTCGAGGT GCTGACCGAG GGCCTTGAGC GGGTGCTGTT GGTGCGCGGT GGTGGCCGTG 3301 AAGTCATCAC CATCTACTCC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3361 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3421 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCAA CCCAAGTTAA 3481 TCTTAAGTGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt