Construct: ORF TRCN0000469908
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017524.1_s317c1
- Derived from:
- ccsbBroadEn_13425
- DNA Barcode:
- GAGCAACTTGATGCCACGTCACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF6 (221458)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469908
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221458 | KIF6 | kinesin family member 6 | NM_001289024.3 | 99.8% | 100% | 426C>G |
| 2 | human | 221458 | KIF6 | kinesin family member 6 | NM_001351566.2 | 89% | 89% | (many diffs) |
| 3 | human | 221458 | KIF6 | kinesin family member 6 | XM_017010428.1 | 41.7% | 41.6% | (many diffs) |
| 4 | human | 221458 | KIF6 | kinesin family member 6 | XM_017010427.1 | 35.4% | 35.3% | (many diffs) |
| 5 | human | 221458 | KIF6 | kinesin family member 6 | NM_001289021.3 | 34.9% | 34.9% | 1_1479del;1905C>G |
| 6 | human | 221458 | KIF6 | kinesin family member 6 | NM_145027.6 | 32.5% | 32.5% | 1_1647del;2073C>G |
| 7 | human | 221458 | KIF6 | kinesin family member 6 | XM_011514358.3 | 31.9% | 31.9% | 1_1647del;2073C>G;2427_2428insTGTTTGGGAAGCAAT |
| 8 | human | 221458 | KIF6 | kinesin family member 6 | XM_005248904.4 | 31% | 30.9% | (many diffs) |
| 9 | human | 221458 | KIF6 | kinesin family member 6 | NM_001289020.3 | 30.4% | 30.4% | 1_1647del;1809_1810ins51;2022C>G |
| 10 | human | 221458 | KIF6 | kinesin family member 6 | XM_011514359.3 | 30.4% | 30.4% | 1_1647del;2073C>G;2179_2180ins51 |
| 11 | human | 221458 | KIF6 | kinesin family member 6 | XM_011514357.3 | 29% | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 861
- ORF length:
- 795
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag agaggaaatg tcattaggat gccaggaggc ttttgaaatc ttcaagaggg 121 accacgctga cagcgttacc atcgatgaca acaaacagat tctgaaacag agattttctg 181 aagccaaggc cctgggagaa agtataaatg aagcaagaag taaaattggt cacctgaagg 241 aagaaatcac ccagcggcat atacagcaag tagccctagg aatctcggaa aacatggccg 301 tgcctctgat gccagaccag caggaggaga agctgcgatc acaactggag gaagaaaaga 361 gaaggtataa aacaatgttc actcgcctga aagccctgaa ggtggagatc gagcacttgc 421 agctgctcat ggacaaagcc aaggtgaagc tacagaaaga gtttgaagtc tggtgggcag 481 aggaggccac gaacctgcag gtaaattctc cagcagtgaa ttcactcgat cacacgaagc 541 catttctcca gacaTCTGAC TCCCAGCATG AATGGTCCCA ACTCCTCTCT AACAAAAGTT 601 CTGGAGGCTG GGAAGTCCAA GATCAAGGCA CTGGCAGATT CGATGTCTGT GATGTGAATG 661 CCAGGAAAAT CCTGCCCTCG CCTTGCCCCA GTCCACACAG CCAGAAACAG AGCAGCACCA 721 GCACCCCACT GGAAGACAGC ATCCCCAAGA GGCCAGTGTC GTCCATCCCT CTCACCGGAG 781 ACAGCCAGAC GGACTCGGAC ATCATCGCCT TCATCAAGGC CAGACAGAGC ATTCTGCAGA 841 AGCAATGTTT GGGAAGCAAT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 901 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 961 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGAGC AACTTGATGC 1021 CACGTCACTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt