Transcript: Human NM_001289024.3

Homo sapiens kinesin family member 6 (KIF6), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
KIF6 (221458)
Length:
7571
CDS:
232..1029

Additional Resources:

NCBI RefSeq record:
NM_001289024.3
NBCI Gene record:
KIF6 (221458)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001289024.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000116580 GTGAAGCTACAGAAAGAGTTT pLKO.1 610 CDS 100% 4.950 3.465 N KIF6 n/a
2 TRCN0000116577 CCAGGAAATATCCATCCATGA pLKO.1 1034 3UTR 100% 4.050 2.835 N KIF6 n/a
3 TRCN0000116581 CTGTGATGTGAATGCCAGGAA pLKO.1 813 CDS 100% 2.640 1.848 N KIF6 n/a
4 TRCN0000074123 CGCCTGTAATCCCAACACTTT pLKO.1 5898 3UTR 100% 4.950 2.475 Y GJD4 n/a
5 TRCN0000166650 CGCCTGTAATCCCAACACTTT pLKO.1 5898 3UTR 100% 4.950 2.475 Y C9orf85 n/a
6 TRCN0000178741 CACACACATACACACACACAA pLKO.1 2771 3UTR 100% 4.950 2.475 Y Cstad n/a
7 TRCN0000107105 CGAGGTCAGGAGTTTGAGATT pLKO.1 5942 3UTR 100% 4.950 2.475 Y NLRP12 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001289024.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13425 pDONR223 100% 99.8% 100% None 426C>G n/a
2 ccsbBroad304_13425 pLX_304 0% 99.8% 100% V5 426C>G n/a
3 TRCN0000469908 GAGCAACTTGATGCCACGTCACTG pLX_317 38.8% 99.8% 100% V5 426C>G n/a
4 ccsbBroadEn_13426 pDONR223 100% 34.9% 34.8% None 0_1ins1479;508T>C n/a
5 ccsbBroad304_13426 pLX_304 0% 34.9% 34.8% V5 0_1ins1479;508T>C n/a
6 TRCN0000468540 CAAGGGTATGTCCTGTGCAACCAA pLX_317 17.7% 34.9% 34.8% V5 0_1ins1479;508T>C n/a
Download CSV