Construct: ORF TRCN0000469930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008368.1_s317c1
- Derived from:
- ccsbBroadEn_10799
- DNA Barcode:
- CCCTGCAGCAGATTCATTATTAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELF2 (1998)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_001276457.1 | 90.5% | 90% | 1355_1356insA;1371_1512del |
| 2 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_001276459.2 | 88.1% | 87.6% | 171_172ins36;1319_1320insA;1335_1476del |
| 3 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_006874.4 | 85.6% | 85.1% | 342_428del;1442_1443insA;1458_1599del |
| 4 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_001276458.2 | 83.3% | 82.9% | (many diffs) |
| 5 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_001371338.1 | 81.8% | 81.3% | 0_1ins132;1223_1224insA;1239_1380del |
| 6 | human | 1998 | ELF2 | E74 like ETS transcription ... | XM_024453924.1 | 81.8% | 81.3% | 0_1ins132;1223_1224insA;1239_1380del |
| 7 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_001371339.1 | 79.4% | 78.9% | (many diffs) |
| 8 | human | 1998 | ELF2 | E74 like ETS transcription ... | XM_024453925.1 | 79.4% | 78.9% | (many diffs) |
| 9 | human | 1998 | ELF2 | E74 like ETS transcription ... | XM_024453926.1 | 79.4% | 78.9% | (many diffs) |
| 10 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_001371336.1 | 79% | 77.3% | (many diffs) |
| 11 | human | 1998 | ELF2 | E74 like ETS transcription ... | XM_011531711.2 | 77.3% | 76.9% | (many diffs) |
| 12 | human | 1998 | ELF2 | E74 like ETS transcription ... | XM_024453918.1 | 77.3% | 76.9% | (many diffs) |
| 13 | human | 1998 | ELF2 | E74 like ETS transcription ... | XM_024453919.1 | 77.3% | 76.9% | (many diffs) |
| 14 | human | 1998 | ELF2 | E74 like ETS transcription ... | XM_024453920.1 | 77.3% | 76.9% | (many diffs) |
| 15 | human | 1998 | ELF2 | E74 like ETS transcription ... | XM_005262805.2 | 76.9% | 75.2% | (many diffs) |
| 16 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_001331036.2 | 75.2% | 73.5% | (many diffs) |
| 17 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_001371337.1 | 75.1% | 74.6% | (many diffs) |
| 18 | human | 1998 | ELF2 | E74 like ETS transcription ... | XM_024453922.1 | 75.1% | 74.6% | (many diffs) |
| 19 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_001371324.1 | 73.2% | 71.5% | (many diffs) |
| 20 | human | 1998 | ELF2 | E74 like ETS transcription ... | NM_201999.3 | 73.2% | 71.5% | (many diffs) |
| 21 | human | 1998 | ELF2 | E74 like ETS transcription ... | XM_011531714.2 | 57% | 56.5% | 0_1ins507;848_849insA;864_1005del |
| 22 | mouse | 69257 | Elf2 | E74-like factor 2 | NM_001291063.1 | 76.4% | 79.2% | (many diffs) |
| 23 | mouse | 69257 | Elf2 | E74-like factor 2 | NM_001291062.1 | 74.2% | 77.1% | (many diffs) |
| 24 | mouse | 69257 | Elf2 | E74-like factor 2 | XM_006502031.4 | 72.7% | 75.5% | (many diffs) |
| 25 | mouse | 69257 | Elf2 | E74-like factor 2 | NM_001356358.1 | 70.6% | 73.4% | (many diffs) |
| 26 | mouse | 69257 | Elf2 | E74-like factor 2 | XM_017319714.2 | 68.3% | 71% | (many diffs) |
| 27 | mouse | 69257 | Elf2 | E74-like factor 2 | NM_023502.2 | 67% | 67.1% | (many diffs) |
| 28 | mouse | 69257 | Elf2 | E74-like factor 2 | XM_030252804.1 | 66.2% | 68.9% | (many diffs) |
| 29 | mouse | 69257 | Elf2 | E74-like factor 2 | XM_030252805.1 | 66.2% | 68.9% | (many diffs) |
| 30 | mouse | 69257 | Elf2 | E74-like factor 2 | NM_001291059.1 | 65.1% | 65.3% | (many diffs) |
| 31 | mouse | 69257 | Elf2 | E74-like factor 2 | XM_006502034.4 | 62.8% | 65.4% | (many diffs) |
| 32 | mouse | 69257 | Elf2 | E74-like factor 2 | XM_030252806.1 | 62.8% | 65.4% | (many diffs) |
| 33 | mouse | 69257 | Elf2 | E74-like factor 2 | XM_030252807.1 | 62.8% | 65.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1437
- ORF length:
- 1371
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gacgtctctg catgagggac ccacgaacca gctggatctg ctcatccggg 121 ccgtggaagc atcagttcac agcagtaatg cacactgtac agataagaca attgaagctg 181 ctgaagccct gcttcatatg gaatctccta cctgcttgag ggattcaaga agtcctgtgg 241 aagtgtttgt tcctccttgt gtatcaactc cagaattcat ccatgctgct atgaggccag 301 atgtcattac agaaactgta gtggaggtgt caactgaaga gtctgaaccc atggatacct 361 ctcctattcc aacatcacca gatagccatg aaccaatgaa aaagaaaaaa ggaaacacaa 421 cctatttgtg ggagtttctt ttagatctac ttcaagataa aaatacttgt cccaggtata 481 ttaaatggac tcagagagaa aaaggcatat tcaagctggt ggattcaaag gctgtctcta 541 agctttgggg aaagcataag aacaaaccag acatgaacta tgaaaccatg ggacgagctt 601 tgagatacta ctaccaaagg ggaattcttg caaaggttga aggacagagg cttgtatatc 661 agttcaagga tatgccgaaa aacatagtgg tcatagatga tgacaaaagt gaaacctgta 721 atgaagattt agcaggaact actgatgaaa aatcattaga acgagtgtca ctgtctgcag 781 aaagtctcct gaaagcagca tcctctgttc gcagtggaaa aaattcatcc cctataaact 841 gctccagagc agagaagggt gtagctagag ttgtgaatat cacttcccct gggcacgatg 901 cttcatccag gtctcctact accactgcat ctgtgtcagc aacagcagct ccaaggacag 961 ttcgtgtggc aatgcaggta cctgttgtaa tgacatcatt GGGTCAGAAA ATTTCAACTG 1021 TGGCAGTTCA GTCAGTTAAT GCAGGTGCAC CATTAATAAC CAGCACTAGT CCAACAACAG 1081 CGACCTCTCC AAAGGTAGTC ATTCAGACAA TCCCTACTGT GATGCCAGCT TCTACTGAAA 1141 ATGGAGACAA AATCACCATG CAGCCTGCCA AAATTATTAC CATCCCAGCT ACACAGCTTG 1201 CACAGTGTCA ACTGCAGACA AAGTCAAATC TGACTGGATC AGGAAGCATT AACATTGTTG 1261 GAACCCCATT GGCTGTGAGA GCACTTACCC CTGTTTCAAT AGCCCATGGT ACACCTGTAA 1321 TGAGACTATC AATGCCTACT CAGCAGGCAT CTGGCCAGAC TCCTCCTCGA GTTATCAGTG 1381 CAGTCATAAA GGGGCCAGAG GTTAAATCGG AAGCAGTGGC AAAAAAAGCA AGAACATGCC 1441 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1501 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1561 ATATATCTTG TGGAAAGGAC GACCCTGCAG CAGATTCATT ATTAGAACGC GTTAAGTCga 1621 caatcaacct ctggattaca aaatttgtga aagatt