Transcript: Human XM_024453919.1

PREDICTED: Homo sapiens E74 like ETS transcription factor 2 (ELF2), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ELF2 (1998)
Length:
3516
CDS:
919..2388

Additional Resources:

NCBI RefSeq record:
XM_024453919.1
NBCI Gene record:
ELF2 (1998)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024453919.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000273855 GTCTCCTGAGTTAGGTATAAA pLKO_005 1176 CDS 100% 15.000 21.000 N ELF2 n/a
2 TRCN0000013859 GCTTTGAGATACTACTACCAA pLKO.1 1405 CDS 100% 3.000 4.200 N ELF2 n/a
3 TRCN0000273913 ATACTTGTCCCAGGTATATTA pLKO_005 1271 CDS 100% 15.000 10.500 N ELF2 n/a
4 TRCN0000304586 ATACTTGTCCCAGGTATATTA pLKO_005 1271 CDS 100% 15.000 10.500 N Elf2 n/a
5 TRCN0000273856 ATGCAGGTGCACCATTAATAA pLKO_005 1847 CDS 100% 15.000 10.500 N ELF2 n/a
6 TRCN0000273916 CATTCTGTAAGAGGTTATATA pLKO_005 2697 3UTR 100% 15.000 10.500 N ELF2 n/a
7 TRCN0000273917 CTATGAGGCCAGATGTCATTA pLKO_005 1010 CDS 100% 13.200 9.240 N ELF2 n/a
8 TRCN0000013862 ACCTGTTGTAATGACATCATT pLKO.1 1788 CDS 100% 5.625 3.938 N ELF2 n/a
9 TRCN0000013858 GCAAGGGAAGTCATCAAGAAA pLKO.1 2446 3UTR 100% 5.625 3.938 N ELF2 n/a
10 TRCN0000013861 GTACACCTGTAATGAGACTAT pLKO.1 2117 CDS 100% 4.950 3.465 N ELF2 n/a
11 TRCN0000013860 CGTAAACCAAAGACCCAGCAA pLKO.1 1138 CDS 100% 2.640 1.848 N ELF2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024453919.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06156 pDONR223 100% 91.6% 91.5% None 0_1ins132;1467A>T n/a
2 ccsbBroad304_06156 pLX_304 0% 91.6% 91.5% V5 0_1ins132;1467A>T n/a
3 TRCN0000471766 CGTTACGCACTCAAGTCTTTGTGC pLX_317 29.2% 91.6% 91.5% V5 0_1ins132;1467A>T n/a
4 ccsbBroadEn_10799 pDONR223 100% 77.3% 76.9% None (many diffs) n/a
5 ccsbBroad304_10799 pLX_304 0% 77.3% 76.9% V5 (many diffs) n/a
6 TRCN0000469930 CCCTGCAGCAGATTCATTATTAGA pLX_317 32.1% 77.3% 76.9% V5 (many diffs) n/a
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