Construct: ORF TRCN0000469943
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002624.1_s317c1
- Derived from:
- ccsbBroadEn_06900
- DNA Barcode:
- GTAAATTCATTACACTTATCCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS17 (6218)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469943
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6218 | RPS17 | ribosomal protein S17 | NM_001021.6 | 99.7% | 99.2% | 5G>C |
| 2 | human | 6218 | RPS17 | ribosomal protein S17 | NR_111944.3 | 66.3% | (many diffs) | |
| 3 | human | 6218 | RPS17 | ribosomal protein S17 | NR_111943.2 | 51.5% | (many diffs) | |
| 4 | human | 79915 | ATAD5 | ATPase family AAA domain co... | XR_001752632.2 | 6.5% | (many diffs) | |
| 5 | human | 79915 | ATAD5 | ATPase family AAA domain co... | XR_001752631.2 | 6.1% | (many diffs) | |
| 6 | human | 79915 | ATAD5 | ATPase family AAA domain co... | XR_934553.3 | 5.9% | (many diffs) | |
| 7 | mouse | 20068 | Rps17 | ribosomal protein S17 | NM_009092.3 | 88.8% | 98.5% | (many diffs) |
| 8 | mouse | 623169 | Gm6402 | predicted pseudogene 6402; ... | NR_030688.1 | 26.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 495
- ORF length:
- 405
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCCGCGT TCGCACCAAA ACCGTGAAGA 121 AGGCGGCCCG GGTCATCATA GAAAAGTACT ACACGCGCCT GGGCAACGAC TTCCACACGA 181 ACAAGCGCGT GTGCGAGGAG ATCGCCATTA TCCCCAGCAA AAAGCTCCGC AACAAGATAG 241 CAGGTTATGT CACGCATCTG ATGAAGCGAA TTCAGAGAGG CCCAGTAAGA GGTATCTCCA 301 TCAAGCTGCA GGAGGAGGAG AGAGAAAGGA GAGACAATTA TGTTCCTGAG GTCTCAGCCT 361 TGGATCAGGA GATTATTGAA GTAGATCCTG ACACTAAGGA AATGCTGAAG CTTTTGGACT 421 TCGGCAGTCT GTCCAACCTT CAGGTCACTC AGCCTACAGT TGGGATGAAT TTCAAAACGC 481 CTCGGGGACC TGTTTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 541 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 601 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTAAATTCAT TACACTTATC 661 CACGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt