Construct: ORF TRCN0000469960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010642.1_s317c1
- Derived from:
- ccsbBroadEn_03410
- DNA Barcode:
- CTGTTTCAGTGGCGGCAATATCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPM3 (54344)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469960
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54344 | DPM3 | dolichyl-phosphate mannosyl... | NM_018973.3 | 100% | 100% | |
| 2 | human | 54344 | DPM3 | dolichyl-phosphate mannosyl... | NM_153741.2 | 75.4% | 75.4% | 0_1ins90 |
| 3 | human | 54344 | DPM3 | dolichyl-phosphate mannosyl... | XM_017001498.1 | 75.4% | 75.4% | 0_1ins90 |
| 4 | mouse | 68563 | Dpm3 | dolichyl-phosphate mannosyl... | NM_026767.4 | 64.2% | 68% | (many diffs) |
| 5 | mouse | 68563 | Dpm3 | dolichyl-phosphate mannosyl... | XM_006501983.1 | 64.2% | 68% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 435
- ORF length:
- 366
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctctccgtg ggcgggcttc ggttgagttt ggtccgcttt tcctttctgc 121 tcctCAGGGG AGCATTGCTT CCTTCTCTCG CAGTGACCAT GACGAAATTA GCGCAGTGGC 181 TTTGGGGACT AGCGATCCTG GGCTCCACCT GGGTGGCCCT GACCACGGGA GCCTTGGGCC 241 TGGAGCTGCC CTTGTCCTGC CAGGAAGTCC TGTGGCCACT GCCCGCCTAC TTGCTGGTGT 301 CCGCCGGCTG CTATGCCCTG GGCACTGTGG GCTATCGTGT GGCCACTTTT CATGACTGCG 361 AGGACGCCGC ACGCGAGCTG CAGAGCCAGA TACAGGAGGC CCGAGCCGAC TTAGCCCGCA 421 GGGGGCTGCG CTTCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 481 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 541 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTGTTTCAGT GGCGGCAATA 601 TCAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt