Construct: ORF TRCN0000469973
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003630.1_s317c1
- Derived from:
- ccsbBroadEn_08535
- DNA Barcode:
- TCAAATCCGGCAATCTTTTCTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSGALNACT2 (55454)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469973
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55454 | CSGALNACT2 | chondroitin sulfate N-acety... | NM_018590.5 | 99.9% | 100% | 18G>T |
| 2 | human | 55454 | CSGALNACT2 | chondroitin sulfate N-acety... | XM_011539938.2 | 80.3% | 76.9% | (many diffs) |
| 3 | human | 55454 | CSGALNACT2 | chondroitin sulfate N-acety... | NM_001319654.1 | 76.6% | 71.1% | 18G>T;1158_1159ins95;1248_1249ins283 |
| 4 | human | 55454 | CSGALNACT2 | chondroitin sulfate N-acety... | XM_017016388.2 | 76.6% | 71.1% | 18G>T;1158_1159ins95;1248_1249ins283 |
| 5 | human | 55454 | CSGALNACT2 | chondroitin sulfate N-acety... | NM_001319656.1 | 63% | 59.6% | 18G>T;979_980ins274;1026_1027ins326 |
| 6 | human | 55454 | CSGALNACT2 | chondroitin sulfate N-acety... | XM_017016389.2 | 63% | 59.6% | 18G>T;979_980ins274;1026_1027ins326 |
| 7 | human | 55454 | CSGALNACT2 | chondroitin sulfate N-acety... | XM_017016391.2 | 54.7% | 54.2% | (many diffs) |
| 8 | human | 55454 | CSGALNACT2 | chondroitin sulfate N-acety... | XR_945783.3 | 41.9% | (many diffs) | |
| 9 | human | 55454 | CSGALNACT2 | chondroitin sulfate N-acety... | NR_135077.1 | 41.2% | (many diffs) | |
| 10 | human | 55454 | CSGALNACT2 | chondroitin sulfate N-acety... | XR_002956986.1 | 38.3% | (many diffs) | |
| 11 | mouse | 78752 | Csgalnact2 | chondroitin sulfate N-acety... | NM_030165.3 | 90.4% | 92.6% | (many diffs) |
| 12 | mouse | 78752 | Csgalnact2 | chondroitin sulfate N-acety... | XM_006506776.3 | 90.4% | 92.6% | (many diffs) |
| 13 | mouse | 78752 | Csgalnact2 | chondroitin sulfate N-acety... | XM_006506777.3 | 90.4% | 92.6% | (many diffs) |
| 14 | mouse | 78752 | Csgalnact2 | chondroitin sulfate N-acety... | XM_006506778.3 | 90.4% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1692
- ORF length:
- 1626
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tagaagagga cttattcttc acacccggac ccactggttg ctgttgggcc 121 ttgctttgct ctgcagtttg gtattattta tgtacctcct ggaatgtgcc ccccagactg 181 atggaaatgc atctcttcct ggtgttgttg gggaaaatta tggtaaagag tattatcaag 241 ccctcctaca ggaacaagaa gaacattatc agaccagggc aaccagtctg aaacgccaaa 301 ttgcccaact aaaacaagaa ttacaagaaa tgagtgagaa gatgcggtca ctgcaagaaa 361 gaaggaatgt aggggctaat ggcataggct atcagagcaa caaagagcaa gcacctagtg 421 atcttttaga gtttcttcat tcccaaattg acaaagctga agttagcata ggggccaaac 481 tacccagtga gtatggggtc attccctttg aaagttttac cttaatgaaa gtatttcaat 541 tggaaatggg tctcactcgc catcctgaag aaaagccagt tagaaaagac aaacgagatg 601 aattggtgga agttattgaa gcgggcttgg aggtcattaa taatcctgat gaagatgatg 661 aacaagaaga tgaggagggt ccccttggag agaaactgat atttaatgaa aatgacttcg 721 tagaaggtta ttatcgcact gagagagata agggcacaca gtatgaactc ttttttaaga 781 aagcagacct tacggaatat agacatgtga ccctcttccg cccttttgga cctctcatga 841 aagtgaagag tgagatgatt gacatcacta gatcaattat taatatcatt gtgccacttg 901 ctgaaagaac tgaagcattt gtacaattta tgcagaactt cagggatgtt tgtattcatc 961 aagacaagaa gattcatctc acagtggtgt attttggtaa agaaggactg tctaaagtca 1021 agtctatcct agaatctgtc accagtgagt ctaattttca caattacacc ttggtctcat 1081 tgaatgaaga atttaatcgt ggacgaggac taaatgtggg tgcccgagct tgggacaagg 1141 gagaggtctt gatgtttttc tgtgatgttg atatctattt ctcagccgaa ttccttaaca 1201 gctgccggtt aaatgctgag ccaggtaaga aggtgtttta cccTGTGGTG TTCAGTCTTT 1261 ACAATCCTGC CATTGTTTAT GCCAACCAGG AAGTGCCACC ACCTGTGGAG CAGCAGCTGG 1321 TTCACAAAAA GGATTCTGGC TTTTGGCGAG ATTTTGGCTT TGGAATGACT TGTCAGTATC 1381 GTTCAGATTT CCTGACCATT GGTGGATTTG ACATGGAAGT GAAAGGTTGG GGTGGAGAAG 1441 ATGTTCATCT TTATCGAAAA TACTTACATG GTGACCTCAT TGTGATTCGG ACTCCGGTTC 1501 CTGGTCTTTT CCACCTCTGG CATGAAAAGC GCTGTGCTGA TGAGCTGACC CCCGAGCAGT 1561 ACCGCATGTG CATCCAGTCT AAAGCCATGA ATGAGGCCTC TCACTCCCAC CTGGGAATGC 1621 TGGTCTTCAG GGAGGAAATA GAGACGCATC TTCATAAACA GGCATACAGG ACAAACAGTG 1681 AAGCTGTTGG TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1741 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1801 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA AATCCGGCAA TCTTTTCTCT 1861 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t