Transcript: Human XR_002956986.1

PREDICTED: Homo sapiens chondroitin sulfate N-acetylgalactosaminyltransferase 2 (CSGALNACT2), transcript variant X5, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CSGALNACT2 (55454)
Length:
3587
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002956986.1
NBCI Gene record:
CSGALNACT2 (55454)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002956986.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000154970 GCATAGGCTATCAGAGCAACA pLKO.1 688 3UTR 100% 4.050 5.670 N CSGALNACT2 n/a
2 TRCN0000151456 CGTTACTGTATGAACCACAAA pLKO.1 1836 3UTR 100% 4.950 3.960 N CSGALNACT2 n/a
3 TRCN0000319332 CGTTACTGTATGAACCACAAA pLKO_005 1836 3UTR 100% 4.950 3.960 N CSGALNACT2 n/a
4 TRCN0000151672 GCTGAAAGAACTGAAGCATTT pLKO.1 1206 3UTR 100% 10.800 7.560 N CSGALNACT2 n/a
5 TRCN0000319331 GCTGAAAGAACTGAAGCATTT pLKO_005 1206 3UTR 100% 10.800 7.560 N CSGALNACT2 n/a
6 TRCN0000150897 GTGGTGTTCAGTCTTTACAAT pLKO.1 1372 3UTR 100% 5.625 3.938 N CSGALNACT2 n/a
7 TRCN0000152477 CGAGATGAATTGGTGGAAGTT pLKO.1 900 3UTR 100% 4.950 3.465 N CSGALNACT2 n/a
8 TRCN0000319330 CGAGATGAATTGGTGGAAGTT pLKO_005 900 3UTR 100% 4.950 3.465 N CSGALNACT2 n/a
9 TRCN0000155785 CCCAGACTGATGGAAATGCAT pLKO.1 478 3UTR 100% 3.000 2.100 N CSGALNACT2 n/a
10 TRCN0000153384 GAGTATTATCAAGCCCTCCTA pLKO.1 534 3UTR 100% 2.640 1.848 N CSGALNACT2 n/a
11 TRCN0000319329 GAGTATTATCAAGCCCTCCTA pLKO_005 534 3UTR 100% 2.640 1.848 N CSGALNACT2 n/a
12 TRCN0000183177 GTGGAGAAGATGTTCATCTTT pLKO.1 1559 3UTR 100% 0.563 0.394 N LOC286453 n/a
13 TRCN0000151072 GCATCTTCATAAACAGGCATA pLKO.1 1773 3UTR 100% 4.050 2.430 N CSGALNACT2 n/a
14 TRCN0000179517 GCATCCAGTCTAAAGCCATAA pLKO.1 1697 3UTR 100% 10.800 7.560 N LOC286453 n/a
15 TRCN0000110462 CCCAGACTGATGGAAATGCTT pLKO.1 478 3UTR 100% 3.000 2.100 N Csgalnact2 n/a
16 TRCN0000288553 CCCAGACTGATGGAAATGCTT pLKO_005 478 3UTR 100% 3.000 2.100 N Csgalnact2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002956986.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08535 pDONR223 100% 38.3% None (many diffs) n/a
2 ccsbBroad304_08535 pLX_304 0% 38.3% V5 (many diffs) n/a
3 TRCN0000469973 TCAAATCCGGCAATCTTTTCTCTC pLX_317 27.8% 38.3% V5 (many diffs) n/a
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