Construct: ORF TRCN0000470010

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000857.1_s317c1
Derived from:
ccsbBroadEn_15408
DNA Barcode:
GGGCTTCAACTTGAAAGATACATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EML1 (2009)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470010
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2009 EML1 EMAP like 1 NM_004434.3 99.9% 99.8% 1654C>A
2 human 2009 EML1 EMAP like 1 XM_005267400.4 98.1% 97.5% (many diffs)
3 human 2009 EML1 EMAP like 1 NM_001008707.2 97.6% 97.6% 381_437del;1711C>A
4 human 2009 EML1 EMAP like 1 XM_005267397.1 97.6% 97.6% 546_602del;1711C>A
5 human 2009 EML1 EMAP like 1 XM_011536540.2 96.6% 96% (many diffs)
6 human 2009 EML1 EMAP like 1 XM_005267398.3 95.8% 95.3% (many diffs)
7 human 2009 EML1 EMAP like 1 XM_017021074.2 94.6% 94.6% 1618_1619ins132
8 human 2009 EML1 EMAP like 1 XM_005267399.4 94.5% 93.8% (many diffs)
9 human 2009 EML1 EMAP like 1 XM_011536541.1 93.9% 93.8% 0_1ins93;453_509del;1618C>A
10 human 2009 EML1 EMAP like 1 XM_011536542.3 89.3% 88.7% (many diffs)
11 human 2009 EML1 EMAP like 1 XM_024449507.1 76.8% 76.8% 0_1ins564;1090C>A
12 mouse 68519 Eml1 echinoderm microtubule asso... NM_001043335.1 86.7% 96% (many diffs)
13 mouse 68519 Eml1 echinoderm microtubule asso... NM_001286346.1 84.9% 93.7% (many diffs)
14 mouse 68519 Eml1 echinoderm microtubule asso... XM_017315170.1 84.9% 94.1% (many diffs)
15 mouse 68519 Eml1 echinoderm microtubule asso... NM_001043336.2 83.1% 92.2% (many diffs)
16 mouse 68519 Eml1 echinoderm microtubule asso... XM_017315172.1 83.1% 92.2% (many diffs)
17 mouse 68519 Eml1 echinoderm microtubule asso... XM_017315169.1 82.7% 90.5% (many diffs)
18 mouse 68519 Eml1 echinoderm microtubule asso... NM_001286347.1 81.4% 90.3% (many diffs)
19 mouse 68519 Eml1 echinoderm microtubule asso... XM_017315171.1 81.4% 90.3% (many diffs)
20 mouse 68519 Eml1 echinoderm microtubule asso... XM_017315168.1 78.5% 86.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2511
ORF length:
2445
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ggacggcttc tccagctaca gcagcctgta cgacacgtcc tcgctgctcc
121agttctgcaa cgatgacagc gcttctgctg caagtagcat ggaggtgaca gaccgcattg
181cttcactgga gcagagagtc cagatgcaag aagacgacat ccagctgctc aaatcagctc
241tagctgatgt ggttcggcgg ctgaacatta ctgaggaaca gcaggccgtg cttaacagga
301aaggacctac caaagcaaga ccactgatgc agaccctgcc tttaagaacc acggtcaaca
361atggcactgt gttaccaaag aaacctactg gctctctacc atccccctcc ggggtcagga
421aagaaactgc tgtgccagca accaaaagta acatcaagag gaccagctct tctgaacgag
481tgtctcctgg gggtcgaagg gaaagcaatg gggattccag aggaaaccgg aatcgcacag
541gctccaccag cagctcttcc agtggcaaaa agaacagtga aagcaaaccc aaggagcctg
601tattcagtgc agaagaaggc tatgtaaaaa tgtttcttcg tggacgccct gttaccatgt
661acatgcccaa agatcaagtg gattcttaca gcttggaagc aaaagtagaa cttccaacca
721agagactcaa gctggaatgg gtctatgggt acaggggtcg agactgccgt aacaacctgt
781acttgcttcc gacgggagag accgtctact tcatcgcatc cgtggtggtg ttatacaacg
841tggaggagca actgcagagg cattacgctg gccacaacga tgacgtgaag tgcctagcag
901ttcatcctga tcggatcacg atagcaacag gacaagttgc gggcacatcg aaggatggaa
961aacaattgcc cccacatgtg cgcatctggg attctgtgac attgaatact ctccacgtca
1021ttggaatagg tttttttgac cgagcagtca cctgtattgc attctcaaaa tctaatggag
1081gaaccaatct ctgtgctgtg gatgactcca acgaccatgt gctctctgta tgggactggc
1141agaaagaaga aaaactagca gatgtgaagt gctctaatga agctgtgttt gctgcggatt
1201tccaccccac ggacaccaac atcatagtta cttgtggaaa atcacatctc tacttttgga
1261cactagaagg aagctccctt aataagaagc aaggattatt cgagaaacaa gaaaagccaa
1321agtttgtcct ctgtgtgact ttctctgaaa acggtgacac cattactgga gattcaagtg
1381gcaacatctt agtatgggga aaaggtacaa atcgaataag ctatgcagtt cagggggccc
1441atgagggtgg catttttgca ctttgtatgt taagagatgg cacactggtg tcgggaggtg
1501ggaaagaccg aaagctcatt tcttggagcg gaaactatca aaaacttcgt aaaacggaga
1561ttccagaaca gtttggtcca atacggacag tggccgaggg gaaaggcgat gtgatcttga
1621ttggcacaac tcgaaacttt gtcctgcagg gcactctgtc aggggacttc acacccatta
1681ctcagggtca cactgatgag ctctggggac tggccatcaa tgcctcaaaa tctcagttct
1741tgacctgtgg gcatgacaag catgccactc tctgggacgc tgtgggtcac cgtcccgtct
1801gggacaaaat aatagaggat ccagctcagt cttctggttt tcatccttca gggtctgtgg
1861ttgcagtcgg aacactcact gggaggtggt ttgtgtttga cacagaaaca aaagacttgg
1921tcaccgttca cacagatgga aacgaacagc tctctgtaat gcgatactca ccagatggga
1981atttcttagc cataggctca catgacaact gcatctatat atatggcgtt agtgacaacg
2041ggaggaagta cacgcgagtg ggcaagtgct cgggtcattc cagcttcatt actcacctgg
2101actggtctgt aaactcacag ttcctcgtgt caaattccgg agactacgaa aTCCTCTACT
2161GGGTTCCCTC TGCCTGTAAG CAAGTCGTAA GTGTGGAAAC TACAAGAGAC ATTGAATGGG
2221CTACCTATAC CTGCACTTTG GGATTCCATG TTTTTGGAGT GTGGCCAGAA GGCTCGGACG
2281GAACCGACAT CAATGCCGTC TGTCGGGCCC ATGAGAAGAA ACTCCTGTCA ACAGGCGACG
2341ACTTTGGCAA AGTGCACCTC TTCTCATACC CCTGCTCGCA GTTCAGGGCT CCAAGCCACA
2401TCTACGGCGG GCACAGCAGC CATGTCACCA ATGTCGATTT CCTCTGTGAA GACAGCCACC
2461TCATCTCCAC GGGCGGGAAA GACACAAGCA TCATGCAGTG GCGCGTCATT TACCCAACTT
2521TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2581CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2641CTTGTGGAAA GGACGAGGGC TTCAACTTGA AAGATACATT ACGCGTTAAG TCgacaatca
2701acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)