Construct: ORF TRCN0000470010
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000857.1_s317c1
- Derived from:
- ccsbBroadEn_15408
- DNA Barcode:
- GGGCTTCAACTTGAAAGATACATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EML1 (2009)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470010
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2009 | EML1 | EMAP like 1 | NM_004434.3 | 99.9% | 99.8% | 1654C>A |
| 2 | human | 2009 | EML1 | EMAP like 1 | XM_005267400.4 | 98.1% | 97.5% | (many diffs) |
| 3 | human | 2009 | EML1 | EMAP like 1 | NM_001008707.2 | 97.6% | 97.6% | 381_437del;1711C>A |
| 4 | human | 2009 | EML1 | EMAP like 1 | XM_005267397.1 | 97.6% | 97.6% | 546_602del;1711C>A |
| 5 | human | 2009 | EML1 | EMAP like 1 | XM_011536540.2 | 96.6% | 96% | (many diffs) |
| 6 | human | 2009 | EML1 | EMAP like 1 | XM_005267398.3 | 95.8% | 95.3% | (many diffs) |
| 7 | human | 2009 | EML1 | EMAP like 1 | XM_017021074.2 | 94.6% | 94.6% | 1618_1619ins132 |
| 8 | human | 2009 | EML1 | EMAP like 1 | XM_005267399.4 | 94.5% | 93.8% | (many diffs) |
| 9 | human | 2009 | EML1 | EMAP like 1 | XM_011536541.1 | 93.9% | 93.8% | 0_1ins93;453_509del;1618C>A |
| 10 | human | 2009 | EML1 | EMAP like 1 | XM_011536542.3 | 89.3% | 88.7% | (many diffs) |
| 11 | human | 2009 | EML1 | EMAP like 1 | XM_024449507.1 | 76.8% | 76.8% | 0_1ins564;1090C>A |
| 12 | mouse | 68519 | Eml1 | echinoderm microtubule asso... | NM_001043335.1 | 86.7% | 96% | (many diffs) |
| 13 | mouse | 68519 | Eml1 | echinoderm microtubule asso... | NM_001286346.1 | 84.9% | 93.7% | (many diffs) |
| 14 | mouse | 68519 | Eml1 | echinoderm microtubule asso... | XM_017315170.1 | 84.9% | 94.1% | (many diffs) |
| 15 | mouse | 68519 | Eml1 | echinoderm microtubule asso... | NM_001043336.2 | 83.1% | 92.2% | (many diffs) |
| 16 | mouse | 68519 | Eml1 | echinoderm microtubule asso... | XM_017315172.1 | 83.1% | 92.2% | (many diffs) |
| 17 | mouse | 68519 | Eml1 | echinoderm microtubule asso... | XM_017315169.1 | 82.7% | 90.5% | (many diffs) |
| 18 | mouse | 68519 | Eml1 | echinoderm microtubule asso... | NM_001286347.1 | 81.4% | 90.3% | (many diffs) |
| 19 | mouse | 68519 | Eml1 | echinoderm microtubule asso... | XM_017315171.1 | 81.4% | 90.3% | (many diffs) |
| 20 | mouse | 68519 | Eml1 | echinoderm microtubule asso... | XM_017315168.1 | 78.5% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2511
- ORF length:
- 2445
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggacggcttc tccagctaca gcagcctgta cgacacgtcc tcgctgctcc 121 agttctgcaa cgatgacagc gcttctgctg caagtagcat ggaggtgaca gaccgcattg 181 cttcactgga gcagagagtc cagatgcaag aagacgacat ccagctgctc aaatcagctc 241 tagctgatgt ggttcggcgg ctgaacatta ctgaggaaca gcaggccgtg cttaacagga 301 aaggacctac caaagcaaga ccactgatgc agaccctgcc tttaagaacc acggtcaaca 361 atggcactgt gttaccaaag aaacctactg gctctctacc atccccctcc ggggtcagga 421 aagaaactgc tgtgccagca accaaaagta acatcaagag gaccagctct tctgaacgag 481 tgtctcctgg gggtcgaagg gaaagcaatg gggattccag aggaaaccgg aatcgcacag 541 gctccaccag cagctcttcc agtggcaaaa agaacagtga aagcaaaccc aaggagcctg 601 tattcagtgc agaagaaggc tatgtaaaaa tgtttcttcg tggacgccct gttaccatgt 661 acatgcccaa agatcaagtg gattcttaca gcttggaagc aaaagtagaa cttccaacca 721 agagactcaa gctggaatgg gtctatgggt acaggggtcg agactgccgt aacaacctgt 781 acttgcttcc gacgggagag accgtctact tcatcgcatc cgtggtggtg ttatacaacg 841 tggaggagca actgcagagg cattacgctg gccacaacga tgacgtgaag tgcctagcag 901 ttcatcctga tcggatcacg atagcaacag gacaagttgc gggcacatcg aaggatggaa 961 aacaattgcc cccacatgtg cgcatctggg attctgtgac attgaatact ctccacgtca 1021 ttggaatagg tttttttgac cgagcagtca cctgtattgc attctcaaaa tctaatggag 1081 gaaccaatct ctgtgctgtg gatgactcca acgaccatgt gctctctgta tgggactggc 1141 agaaagaaga aaaactagca gatgtgaagt gctctaatga agctgtgttt gctgcggatt 1201 tccaccccac ggacaccaac atcatagtta cttgtggaaa atcacatctc tacttttgga 1261 cactagaagg aagctccctt aataagaagc aaggattatt cgagaaacaa gaaaagccaa 1321 agtttgtcct ctgtgtgact ttctctgaaa acggtgacac cattactgga gattcaagtg 1381 gcaacatctt agtatgggga aaaggtacaa atcgaataag ctatgcagtt cagggggccc 1441 atgagggtgg catttttgca ctttgtatgt taagagatgg cacactggtg tcgggaggtg 1501 ggaaagaccg aaagctcatt tcttggagcg gaaactatca aaaacttcgt aaaacggaga 1561 ttccagaaca gtttggtcca atacggacag tggccgaggg gaaaggcgat gtgatcttga 1621 ttggcacaac tcgaaacttt gtcctgcagg gcactctgtc aggggacttc acacccatta 1681 ctcagggtca cactgatgag ctctggggac tggccatcaa tgcctcaaaa tctcagttct 1741 tgacctgtgg gcatgacaag catgccactc tctgggacgc tgtgggtcac cgtcccgtct 1801 gggacaaaat aatagaggat ccagctcagt cttctggttt tcatccttca gggtctgtgg 1861 ttgcagtcgg aacactcact gggaggtggt ttgtgtttga cacagaaaca aaagacttgg 1921 tcaccgttca cacagatgga aacgaacagc tctctgtaat gcgatactca ccagatggga 1981 atttcttagc cataggctca catgacaact gcatctatat atatggcgtt agtgacaacg 2041 ggaggaagta cacgcgagtg ggcaagtgct cgggtcattc cagcttcatt actcacctgg 2101 actggtctgt aaactcacag ttcctcgtgt caaattccgg agactacgaa aTCCTCTACT 2161 GGGTTCCCTC TGCCTGTAAG CAAGTCGTAA GTGTGGAAAC TACAAGAGAC ATTGAATGGG 2221 CTACCTATAC CTGCACTTTG GGATTCCATG TTTTTGGAGT GTGGCCAGAA GGCTCGGACG 2281 GAACCGACAT CAATGCCGTC TGTCGGGCCC ATGAGAAGAA ACTCCTGTCA ACAGGCGACG 2341 ACTTTGGCAA AGTGCACCTC TTCTCATACC CCTGCTCGCA GTTCAGGGCT CCAAGCCACA 2401 TCTACGGCGG GCACAGCAGC CATGTCACCA ATGTCGATTT CCTCTGTGAA GACAGCCACC 2461 TCATCTCCAC GGGCGGGAAA GACACAAGCA TCATGCAGTG GCGCGTCATT TACCCAACTT 2521 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2581 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2641 CTTGTGGAAA GGACGAGGGC TTCAACTTGA AAGATACATT ACGCGTTAAG TCgacaatca 2701 acctctggat tacaaaattt gtgaaagatt