Transcript: Human XM_017021074.2

PREDICTED: Homo sapiens EMAP like 1 (EML1), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
EML1 (2009)
Length:
3811
CDS:
70..2385

Additional Resources:

NCBI RefSeq record:
XM_017021074.2
NBCI Gene record:
EML1 (2009)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017021074.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000116291 GCATCTATATATATGGCGTTA pLKO.1 1883 CDS 100% 4.050 5.670 N EML1 n/a
2 TRCN0000331344 TCATGCAGTGGCGCGTCATTT pLKO_005 2363 CDS 100% 13.200 10.560 N EML1 n/a
3 TRCN0000116288 CCAGAACAGTTTGGTCCAATA pLKO.1 1567 CDS 100% 10.800 8.640 N EML1 n/a
4 TRCN0000300287 CCAGAACAGTTTGGTCCAATA pLKO_005 1567 CDS 100% 10.800 8.640 N EML1 n/a
5 TRCN0000116287 GCCACCTTCTTGTGTATATTA pLKO.1 3147 3UTR 100% 15.000 10.500 N EML1 n/a
6 TRCN0000300289 GCCACCTTCTTGTGTATATTA pLKO_005 3147 3UTR 100% 15.000 10.500 N EML1 n/a
7 TRCN0000116289 GCAGATGTGAAGTGCTCTAAT pLKO.1 1162 CDS 100% 13.200 9.240 N EML1 n/a
8 TRCN0000300288 GCAGATGTGAAGTGCTCTAAT pLKO_005 1162 CDS 100% 13.200 9.240 N EML1 n/a
9 TRCN0000310799 TCGCATCCGTGGTGGTGTTAT pLKO_005 818 CDS 100% 13.200 9.240 N EML1 n/a
10 TRCN0000116290 CCAAAGATCAAGTGGATTCTT pLKO.1 671 CDS 100% 5.625 3.938 N EML1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017021074.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15408 pDONR223 0% 94.6% 94.6% None 1618_1619ins132 n/a
2 ccsbBroad304_15408 pLX_304 0% 94.6% 94.6% V5 1618_1619ins132 n/a
3 TRCN0000470010 GGGCTTCAACTTGAAAGATACATT pLX_317 15% 94.6% 94.6% V5 1618_1619ins132 n/a
4 ccsbBroadEn_06157 pDONR223 100% 94.5% 94.6% None 276T>C;1618_1619ins132 n/a
5 ccsbBroad304_06157 pLX_304 0% 94.5% 94.6% V5 276T>C;1618_1619ins132 n/a
6 TRCN0000481317 AAGGGGCAAGAAATCACCGCTTGG pLX_317 21.7% 94.5% 94.6% V5 276T>C;1618_1619ins132 n/a
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