Construct: ORF TRCN0000470016
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006884.1_s317c1
- Derived from:
- ccsbBroadEn_11374
- DNA Barcode:
- CCGTGTAAGATCCGAATACCGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMF1 (9491)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470016
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_006814.5 | 69.8% | 56% | (many diffs) |
| 2 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_178578.3 | 69.8% | 56% | (many diffs) |
| 3 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_001323407.2 | 69.5% | 57.4% | (many diffs) |
| 4 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_001323410.2 | 65.4% | 50.3% | (many diffs) |
| 5 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_001323408.2 | 65.3% | 51.5% | (many diffs) |
| 6 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_001323409.2 | 65% | 50.1% | (many diffs) |
| 7 | mouse | 228769 | Psmf1 | proteasome (prosome, macrop... | NM_001305244.1 | 62.2% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 732
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc taagaagctt gggcccaaat ctcagagaca gcgtggggct gttgcccccc 121 tcacccaggt ccagcaggcg gcctgttccc ccacttataa acaggccaag cgcaatgcca 181 ggagctatca gggccgccgc cgccgccgtc gttgcagcca gaataccact tccagggttc 241 ctagccaact gcaagcagtt gactcttcat ctgcttcctg caggacctac aagaacagtg 301 aggagcttcg gTCTCGTATT GTGTCTGGAA TCATCACACC TATCCATGAG CAGTGGGAAA 361 AGGCTAATGT AAGCAGTCCC CACCGGGAGT TCCCCCCTGC TACCGCCAGA GAGGTGGACC 421 CACTCCGGAT TCCTCCACAC CACCCACACA CCAGTCGGCA GCCTCCCTGG TGTGATCCCC 481 TGGGCCCGTT TGTTGTCGGG GGAGAAGACT TAGACCCTTT TGGGCCTCGG AGAGGTGGCA 541 TGATTGTGGA TCCCCTGAGA TCTGGCTTCC CAAGAGCACT TATTGACCCT TCCTCAGGCC 601 TCCCGAACCG ACTTCCTCCA GGCGCTGTGC CCCCAGGAGC TCGCTTTGAC CCCTTTGGAC 661 CCATTGGGAC CAGCCCACCC GGACCTAACC CAGACCATCT CCCCCCGCCG GGCTACGATG 721 ACATGTACCT GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCG TGTAAGATCC GAATACCGCA 901 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t